| Identification |
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| Name | Mitosis inhibitor protein kinase SWE1 |
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| Synonyms | |
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| Gene Name | SWE1 |
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| Enzyme Class | Not Available |
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| Biological Properties |
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| General Function | regulation of meiotic nuclear division |
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| Specific Function | Protein kinase that acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylating and inhibiting the mitosis-promoting cyclin B-bound CDC28 at 'Tyr-19'. SWE1-mediated inhibition of CDC28 acts in a cell size or morphogenesis checkpoint to delay mitosis in response to defects in growth, actin organization or bud formation. Inhibits the activity of B-type cyclins in replication initiation strongly for CLB2, moderately for CLB3 and CLB4, and there is no apparent inhibition for CLB5 and CLB6, correlating with the normal expression timing of those cyclins. Hyperphosphorylation and degradation of SWE1 when all checkpoint requirement are met releases CLB2-CDC28 from inhibition and allows for progression through the cell cycle. SWE1-dependent CDC28 phosphorylation is also required for pachytene arrest upon activation of the recombination checkpoint during meiosis. Also involved in the regulation of nitrogen starvation- and short chain alcohol-induced filamentous growth, or filamentous differentiation in response to slowed DNA synthesis. Can act both on serines and on tyrosines. |
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| Cellular Location | Not Available |
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| SMPDB Pathways | | Stress-activated signalling pathways: high osmolarity test 1 | PW002773 |    |
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| KEGG Pathways | Not Available |
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| SMPDB Reactions | Not Available |
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| KEGG Reactions | Not Available |
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| Metabolites | |
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| GO Classification | | Function |
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| protein serine/threonine kinase activity | | cell division | | protein phosphorylation | | protein tyrosine kinase activity | | mitotic nuclear division | | regulation of cyclin-dependent protein serine/threonine kinase activity | | G2/M transition of mitotic cell cycle | | meiotic cell cycle | | morphogenesis checkpoint | | negative regulation of spindle pole body separation | | positive regulation of sphingolipid biosynthetic process | | re-entry into mitotic cell cycle | | metal ion binding | | regulation of meiotic nuclear division | | ATP binding | | nucleus | | cellular bud neck |
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| Gene Properties |
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| Chromosome Location | Not Available |
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| Locus | |
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| Gene Sequence | >2460 bp
ATGAGTTCTTTGGACGAGGATGAAGAGGACTTCGAAATGCTGGACACGGAGAACCTCCAG
TTTATGGGGAAGAAGATGTTTGGCAAACAGGCCGGCGAAGACGAGAGTGATGATTTTGCT
ATAGGGGGTAGCACCCCGACCAATAAACTGAAATTTTATCCATATTCGAACAACAAATTG
ACAAGAAGTACGGGGACCTTGAACCTGTCATTAAGTAATACAGCTTTGTCAGAGGCTAAC
TCCAAATTTCTTGGGAAAATTGAAGAAGAGGAAGAAGAGGAGGAAGAAGGCAAGGATGAG
GAAAGCGTGGATTCTCGTATTAAAAGGTGGTCTCCGTTCCATGAAAATGAAAGTGTTACT
ACTCCTATTACAAAAAGATCTGCGGAAAAAACGAACAGTCCTATTTCTCTCAAACAATGG
AACCAGCGATGGTTTCCGAAAAATGATGCTCGCACTGAAAATACATCCTCATCCTCTTCA
TATAGCGTCGCTAAACCTAACCAATCAGCCTTTACGTCTTCGGGCCTCGTATCTAAAATG
TCTATGGACACTTCGTTATACCCTGCGAAATTGAGGATACCAGAAACACCAGTGAAAAAA
TCACCCTTAGTGGAGGGAAGAGACCATAAGCATGTCCACCTTTCGAGTTCGAAAAATGCA
TCGTCTTCTCTAAGTGTTTCCCCTTTAAATTTTGTTGAAGACAATAATTTACAAGAAGAC
CTTTTATTTTCAGATTCTCCGTCTTCGAAAGCTTTACCTTCCATCCATGTACCAACCATA
GACTCATCCCCACTGAGCGAGGCAAAATATCATGCACATGATCGTCACAATAACCAGACA
AACATCCTGTCTCCCACTAATAGCTTGGTTACCAACAGCTCTCCACAAACATTGCATTCT
AACAAGTTCAAAAAAATCAAAAGAGCAAGGAATTCGGTTATTTTGAAAAATAGAGAGCTA
ACAAACAGTTTACAACAATTCAAAGATGATTTATACGGCACGGACGAGAATTTCCCACCT
CCAATCATAATATCAAGTCATCATTCAACTAGAAAGAACCCTCAACCTTATCAATTTCGT
GGACGCTATGACAATGACACTGACGAAGAGATCTCCACTCCAACAAGACGAAAATCTATT
ATTGGGGCAACATCTCAAACACATAGAGAAAGCAGACCATTGTCACTCTCCTCTGCCATC
GTGACAAACACAACAAGTGCAGAGACGCATTCCATATCTTCCACCGATTCTTCGCCGTTA
AATTCCAAAAGGCGTCTAATCTCTTCAAATAAGTTATCAGCAAATCCAGATTCCCATCTT
TTCGAAAAATTTACGAATGTGCATTCCATTGGTAAAGGCCAGTTTTCCACGGTCTACCAG
GTTACGTTTGCCCAAACAAACAAAAAGTATGCAATCAAAGCCATTAAACCAAACAAATAT
AATTCCTTGAAACGCATATTACTGGAAATTAAAATACTAAACGAGGTAACAAACCAAATT
ACAATGGATCAAGAAGGGAAGGAATACATCATCGATTACATCAGTTCCTGGAAGTTTCAA
AATTCATACTATATTATGACAGAATTGTGCGAAAATGGTAATTTGGATGGATTTTTACAA
GAGCAAGTTATCGCAAAGAAAAAAAGGTTGGAAGATTGGAGAATTTGGAAAATCATCGTG
GAATTAAGCCTGGCTTTACGATTCATCCATGATTCTTGTCACATTGTGCATCTGGACTTG
AAACCCGCAAACGTCATGATCACATTTGAAGGTAACCTAAAACTAGGTGACTTTGGAATG
GCTACTCATTTACCGTTGGAGGATAAAAGTTTTGAAAATGAAGGTGACAGAGAATATATT
GCACCAGAAATCATTTCTGATTGTACGTACGATTACAAGGCAGATATTTTTTCCCTGGGT
CTGATGATTGTTGAAATTGCAGCGAACGTTGTGTTACCTGACAATGGCAACGCATGGCAT
AAGTTGAGATCGGGTGATTTATCGGATGCAGGAAGATTAAGTTCCACAGATATTCATTCT
GAATCATTATTTTCAGACATTACGAAAGTAGATACAAATGATTTATTTGATTTTGAAAGA
GACAATATCAGTGGTAATAGTAACAACGCTGGCACCTCCACTGTTCATAACAATAGTAAT
ATCAACAACCCTAATATGAATAATGGCAACGATAATAATAATGTCAATACTGCCGCTACC
AAGAATCGTCTTATTTTGCATAAAAGTTCTAAAATTCCCGCATGGGTACCGAAATTTCTT
ATTGATGGTGAATCACTTGAGAGAATAGTACGATGGATGATAGAGCCCAATTATGAGAGA
AGGCCCACGGCAAATCAAATCTTACAAACTGAGGAATGCCTGTATGTAGAAATGACACGC
AATGCAGGTGCTATTATCCAGGAAGACGACTTTGGACCTAAGCCAAAATTTTTTATATGA
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| Protein Properties |
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| Pfam Domain Function | Not Available |
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| Protein Residues | 819 |
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| Protein Molecular Weight | 92467.12 |
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| Protein Theoretical pI | Not Available |
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| Signalling Regions | Not Available |
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| Transmembrane Regions | Not Available |
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| Protein Sequence | >Mitosis inhibitor protein kinase SWE1
MSSLDEDEEDFEMLDTENLQFMGKKMFGKQAGEDESDDFAIGGSTPTNKLKFYPYSNNKL
TRSTGTLNLSLSNTALSEANSKFLGKIEEEEEEEEEGKDEESVDSRIKRWSPFHENESVT
TPITKRSAEKTNSPISLKQWNQRWFPKNDARTENTSSSSSYSVAKPNQSAFTSSGLVSKM
SMDTSLYPAKLRIPETPVKKSPLVEGRDHKHVHLSSSKNASSSLSVSPLNFVEDNNLQED
LLFSDSPSSKALPSIHVPTIDSSPLSEAKYHAHDRHNNQTNILSPTNSLVTNSSPQTLHS
NKFKKIKRARNSVILKNRELTNSLQQFKDDLYGTDENFPPPIIISSHHSTRKNPQPYQFR
GRYDNDTDEEISTPTRRKSIIGATSQTHRESRPLSLSSAIVTNTTSAETHSISSTDSSPL
NSKRRLISSNKLSANPDSHLFEKFTNVHSIGKGQFSTVYQVTFAQTNKKYAIKAIKPNKY
NSLKRILLEIKILNEVTNQITMDQEGKEYIIDYISSWKFQNSYYIMTELCENGNLDGFLQ
EQVIAKKKRLEDWRIWKIIVELSLALRFIHDSCHIVHLDLKPANVMITFEGNLKLGDFGM
ATHLPLEDKSFENEGDREYIAPEIISDCTYDYKADIFSLGLMIVEIAANVVLPDNGNAWH
KLRSGDLSDAGRLSSTDIHSESLFSDITKVDTNDLFDFERDNISGNSNNAGTSTVHNNSN
INNPNMNNGNDNNNVNTAATKNRLILHKSSKIPAWVPKFLIDGESLERIVRWMIEPNYER
RPTANQILQTEECLYVEMTRNAGAIIQEDDFGPKPKFFI |
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| References |
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| External Links | | Resource | Link |
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| Saccharomyces Genome Database | SWE1 | | Uniprot ID | P32944 | | Uniprot Name | SWE1 |
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| General Reference | Not Available |
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