Cystathionine beta-synthase (P32582) - Yeast Metabolome Database

Identification

Name

Cystathionine beta-synthase

Synonyms

Beta-thionase
Serine sulfhydrase
Sulfur transfer protein 4

Gene Name

CYS4

Enzyme Class

4.2.1.22

Biological Properties

General Function

Involved in cysteine biosynthetic process from serine

Specific Function

L-serine + L-homocysteine = L-cystathionine + H(2)O

Cellular Location

Not Available

SMPDB Pathways

Cysteine Metabolism	PW002383
Selenocompound metabolism	PW002472
Sulfur metabolism	PW002483

KEGG Pathways

Cysteine and methionine metabolism	ec00270
Glycine, serine and threonine metabolism	ec00260
Selenocompound metabolism	ec00450
Sulfur metabolism	ec00920

SMPDB Reactions

Homocysteine + L-Serine → water + L-Cystathionine

KEGG Reactions

L-Homocysteine + L-Serine → L-Cystathionine + water

Metabolites

YMDB ID	Name	View
YMDB00112	L-Serine	Show
YMDB00245	L-Cystathionine	Show
YMDB00371	Homocysteine	Show
YMDB00520	L-Homocysteine	Show
YMDB00890	water	Show

GO Classification

Component
intracellular part
cytoplasm
cell part
Function
cofactor binding
pyridoxal phosphate binding
carbon-oxygen lyase activity
hydro-lyase activity
catalytic activity
lyase activity
cystathionine beta-synthase activity
binding
Process
serine family amino acid metabolic process
L-serine metabolic process
sulfur amino acid metabolic process
metabolic process
sulfur amino acid biosynthetic process
cysteine biosynthetic process from serine
cellular metabolic process
cysteine biosynthetic process
cellular amino acid and derivative metabolic process
cysteine biosynthetic process via cystathionine
cellular amino acid metabolic process

Gene Properties

Chromosome Location

chromosome 7

Locus

YGR155W

Gene Sequence

>1524 bp ATGACTAAATCTGAGCAGCAAGCCGATTCAAGACATAACGTTATCGACTTAGTTGGTAAC ACCCCATTGATCGCACTGAAAAAATTGCCTAAGGCTTTGGGTATCAAACCACAAATTTAT GCTAAGCTGGAACTATACAATCCAGGTGGTTCCATCAAAGACAGAATTGCCAAGTCTATG GTGGAAGAAGCTGAAGCTTCCGGTAGAATTCATCCTTCCAGATCTACTCTGATCGAACCT ACTTCTGGTAACACCGGTATCGGTCTAGCTTTAATCGGCGCCATCAAAGGTTACAGAACT ATCATCACCTTGCCGGAAAAAATGTCTAACGAGAAAGTTTCTGTCCTAAAGGCTCTGGGT GCTGAAATCATCAGAACTCCAACTGCTGCTGCCTGGGATTCTCCAGAATCACATATTGGT GTTGCTAAGAAGTTGGAAAAAGAGATTCCTGGTGCTGTTATACTTGACCAATATAACAAT ATGATGAACCCAGAAGCTCATTACTTTGGTACTGGTCGCGAAATCCAAAGACAGCTAGAA GACTTGAATTTATTTGATAATCTACGCGCTGTTGTTGCTGGTGCTGGTACTGGTGGGACT ATTAGCGGTATTTCCAAGTACTTGAAAGAACAGAATGATAAGATCCAAATCGTTGGTGCT GACCCATTCGGTTCAATTTTAGCCCAACCTGAAAACTTGAATAAGACTGATATCACTGAC TACAAAGTTGAGGGTATTGGTTATGATTTTGTTCCTCAGGTTTTGGACAGAAAATTAATT GATGTTTGGTATAAGACAGACGACAAGCCTTCTTTCAAATACGCCAGACAATTGATTTCT AACGAAGGTGTCTTGGTGGGTGGTTCTTCCGGTTCTACCTTCACTGCGGTTGTGAAATAC TGTGAAGACCACCCTGAACTGACTGAAGATGATGTCATTGTTGCCATATTCCCAGATTCC ATCAGGTCGTACCTAACCAAATTCGTCGATGACGAATGGTTGAAAAAGAACAATTTGTGG GATGATGACGTGTTGGCCCGTTTTGACTCTTCAAAGCTGGAGGCTTCGACGACAAAATAC GCTGATGTGTTTGGTAACGCTACTGTAAAGGATCTTCACTTGAAACCGGTTGTTTCCGTT AAGGAAACCGCTAAGGTCACTGATGTTATCAAGATATTAAAAGACAATGGCTTTGACCAA TTGCCTGTGTTGACTGAAGACGGCAAGTTGTCTGGTTTAGTTACTCTCTCTGAGCTTCTA AGAAAACTATCAATCAATAATTCAAACAACGACAACACTATAAAGGGTAAATACTTGGAC TTCAAGAAATTAAACAATTTCAATGATGTTTCCTCTTACAACGAAAATAAATCCGGTAAG AAGAAGTTTATTAAATTCGATGAAAACTCAAAGCTATCTGACTTGAATCGTTTCTTTGAA AAAAACTCATCTGCCGTTATCACTGATGGCTTGAAACCAATCCATATCGTTACTAAGATG GATTTACTGAGCTACTTAGCATAA

Protein Properties

Pfam Domain Function

PALP (PF00291 )
CBS (PF00571 )

Protein Residues

507

Protein Molecular Weight

56021.30078

Protein Theoretical pI

6.66

Signalling Regions

None

Transmembrane Regions

None

Protein Sequence

>Cystathionine beta-synthase MTKSEQQADSRHNVIDLVGNTPLIALKKLPKALGIKPQIYAKLELYNPGGSIKDRIAKSM VEEAEASGRIHPSRSTLIEPTSGNTGIGLALIGAIKGYRTIITLPEKMSNEKVSVLKALG AEIIRTPTAAAWDSPESHIGVAKKLEKEIPGAVILDQYNNMMNPEAHYFGTGREIQRQLE DLNLFDNLRAVVAGAGTGGTISGISKYLKEQNDKIQIVGADPFGSILAQPENLNKTDITD YKVEGIGYDFVPQVLDRKLIDVWYKTDDKPSFKYARQLISNEGVLVGGSSGSAFTAVVKY CEDHPELTEDDVIVAIFPDSIRSYLTKFVDDEWLKKNNLWDDDVLARFDSSKLEASTTKY ADVFGNATVKDLHLKPVVSVKETAKVTDVIKILKDNGFDQLPVLTEDGKLSGLVTLSELL RKLSINNSNNDNTIKGKYLDFKKLNNFNDVSSYNENKSGKKKFIKFDENSKLSDLNRFFE KNSSAVITDGLKPIHIVTKMDLLSYLA

References

External Links

Resource	Link
Saccharomyces Genome Database	CYS4
Uniprot ID	P32582
Uniprot Name	CBS_YEAST
GenBank Gene ID	D16502
Genebank Protein ID	416161

General Reference

Cherest, H., Thomas, D., Surdin-Kerjan, Y. (1993). "Cysteine biosynthesis in Saccharomyces cerevisiae occurs through the transsulfuration pathway which has been built up by enzyme recruitment." J Bacteriol 175:5366-5374.8366024
Kruger, W. D., Cox, D. R. (1994). "A yeast system for expression of human cystathionine beta-synthase: structural and functional conservation of the human and yeast genes." Proc Natl Acad Sci U S A 91:6614-6618.8022826
Skala, J., Nawrocki, A., Goffeau, A. (1995). "The sequence of a 27 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2, NSR1 genes and ten new open reading frames." Yeast 11:1421-1427.8585325
Tettelin, H., Agostoni Carbone, M. L., Albermann, K., Albers, M., Arroyo, J., Backes, U., Barreiros, T., Bertani, I., Bjourson, A. J., Bruckner, M., Bruschi, C. V., Carignani, G., Castagnoli, L., Cerdan, E., Clemente, M. L., Coblenz, A., Coglievina, M., Coissac, E., Defoor, E., Del Bino, S., Delius, H., Delneri, D., de Wergifosse, P., Dujon, B., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Nature 387:81-84.9169869
Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956