V-type proton ATPase subunit a, vacuolar isoform (P32563)

Identification
NameV-type proton ATPase subunit a, vacuolar isoform
Synonyms
  • V-ATPase a 1 subunit
  • V-ATPase 95 kDa subunit
  • Vacuolar pH protein 1
  • Vacuolar proton pump a subunit
  • Vacuolar proton translocating ATPase subunit a 1
Gene NameVPH1
Enzyme ClassNot Available
Biological Properties
General FunctionEnergy production and conversion
Specific FunctionSubunit of the integral membrane V0 complex of vacuolar ATPase essential for assembly and catalytic activity. Is present only in vacuolar V-ATPase complexes. Enzymes containing this subunit have a 4-fold higher ratio of proton transport to ATP hydrolysis than complexes containing the Golgi/endosomal isoform and undergo reversible dissociation of V1 and V0 in response to glucose depletion. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells
Cellular LocationVacuole membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
GO Classification
Component
macromolecular complex
protein complex
proton-transporting two-sector ATPase complex, proton-transporting domain
Function
transporter activity
transmembrane transporter activity
substrate-specific transmembrane transporter activity
ion transmembrane transporter activity
cation transmembrane transporter activity
inorganic cation transmembrane transporter activity
monovalent inorganic cation transmembrane transporter activity
hydrogen ion transmembrane transporter activity
Process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleoside phosphate metabolic process
nucleotide metabolic process
purine nucleotide metabolic process
metabolic process
purine nucleotide biosynthetic process
purine nucleoside triphosphate biosynthetic process
purine ribonucleoside triphosphate biosynthetic process
ATP biosynthetic process
ATP synthesis coupled proton transport
nitrogen compound metabolic process
cellular nitrogen compound metabolic process
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>2523 bp ATGGCAGAGAAGGAGGAAGCGATTTTTCGCTCTGCTGAAATGGCTTTAGTCCAATTCTAT ATTCCTCAAGAAATTTCAAGAGACTCTGCTTACACTTTAGGTCAATTGGGTCTTGTTCAA TTCCGTGACTTGAACTCTAAGGTGCGTGCGTTTCAAAGAACTTTCGTGAACGAAATTAGA AGACTGGATAATGTAGAAAGACAGTATCGTTACTTTTATTCTCTTTTGAAGAAACACGAT ATTAAGCTCTACGAAGGAGACACGGACAAATATTTGGACGGCTCAGGTGAATTGTACGTT CCACCAAGCGGTTCAGTGATAGATGATTATGTCCGGAACGCTTCATATTTGGAAGAAAGA TTGATTCAAATGGAGGATGCAACCGATCAAATTGAAGTCCAGAAAAATGACTTGGAACAG TATCGCTTTATTTTGCAGTCAGGTGATGAATTTTTCTTGAAGGGTGATAATACCGACAGC ACTTCCTATATGGATGAAGACATGATCGACGCTAATGGGGAAAACATTGCTGCTGCTATC GGTGCTTCTGTAAACTATGTCACTGGTGTCATTGCTAGAGACAAAGTTGCCACCTTAGAA CAAATTCTTTGGAGAGTATTAAGAGGTAACCTTTTCTTCAAAACTGTTGAAATTGAACAA CCTGTTTATGATGTCAAAACCAGGGAGTATAAACATAAAAATGCTTTTATCGTATTTTCT CACGGTGATCTGATTATTAAAAGAATCAGAAAGATTGCGGAATCATTGGATGCCAATCTT TACGATGTTGACTCTTCCAACGAGGGTAGATCACAACAATTGGCCAAGGTCAACAAGAAT TTGAGTGATTTGTACACAGTTTTGAAAACCACTTCTACCACTTTAGAAAGTGAATTATAT GCCATTGCCAAAGAATTGGACTCTTGGTTCCAAGATGTTACCCGTGAAAAGGCGATTTTT GAAATTTTGAACAAGTCTAACTATGATACCAATAGAAAGATTTTGATTGCTGAAGGTTGG ATACCAAGAGACGAATTGGCTACTTTGCAAGCTCGTCTTGGTGAAATGATCGCAAGATTG GGTATTGATGTCCCATCCATTATCCAAGTCCTGGATACAAACCACACTCCACCTACCTTC CACAGAACTAACAAGTTTACTGCTGGTTTCCAAAGTATCTGTGACTGTTACGGTATTGCT CAGTACAGAGAAATCAATGCTGGTTTACCCACAATTGTCACTTTCCCTTTCATGTTTGCC ATCATGTTTGGTGATATGGGTCACGGGTTCTTAATGACCTTAGCCGCATTGTCTCTTGTA TTGAATGAAAAGAAAATCAACAAAATGAAAAGAGGCGAAATTTTCGATATGGCCTTCACT GGTAGATACATTATTTTGTTGATGGGTGTCTTTTCCATGTACACAGGTTTCCTTTACAAC GATATCTTCTCTAAAACTATGACTATTTTCAAGTCTGGTTGGAAATGGCCTGATCATTGG AAAAAAGGTGAGAGTATTACTGCTACATCGGTGGGTACATACCCTATCGGTTTAGATTGG GCTTGGCATGGAACTGAAAATGCTTTGTTATTTTCTAATTCTTACAAAATGAAACTATCA ATTTTAATGGGGTTCATCCACATGACCTATTCTTATTTCTTTTCGTTGGCTAACCACCTA TACTTTAACTCTATGATTGATATCATCGGTAACTTTATTCCTGGTTTGCTATTTATGCAA GGTATCTTTGGTTATCTTTCCGTTTGTATTGTTTACAAATGGGCTGTTGATTGGGTTAAG GACGGAAAGCCTGCTCCAGGTTTGTTAAATATGTTGATCAACATGTTTTTATCACCAGGA ACTATTGACGATGAATTATACCCTCATCAAGCAAAGGTCCAAGTGTTTTTGTTGTTGATG GCCTTGGTTTGTATTCCTTGGTTGCTATTGGTGAAGCCATTACATTTCAAATTCACTCAT AAAAAGAAATCTCACGAACCACTGCCATCGACTGAAGCAGATGCTAGTTCTGAAGATTTG GAAGCACAACAATTAATTTCCGCGATGGACGCCGATGACGCTGAAGAAGAAGAAGTTGGT TCTGGATCTCATGGTGAAGACTTTGGTGATATTATGATTCATCAAGTTATTCATACAATT GAATTCTGTTTGAATTGTGTTTCGCACACTGCATCCTATTTACGTTTATGGGCCTTATCA TTGGCACATGCTCAATTGTCTAGTGTTTTATGGACAATGACAATTCAAATTGCCTTTGGA TTTAGAGGATTTGTGGGTGTGTTTATGACGGTTGCACTTTTTGCCATGTGGTTCGCACTA ACATGTGCAGTTCTTGTTTTGATGGAAGGTACATCTGCCATGCTTCATTCCTTACGTTTG CACTGGGTTGAATCTATGTCCAAGTTTTTCGTGGGTGAAGGTTTACCATACGAACCATTC GCATTTGAGTATAAAGACATGGAAGTCGCTGTTGCTAGTGCAAGCTCTTCCGCTTCAAGC TAA
Protein Properties
Pfam Domain Function
Protein Residues840
Protein Molecular Weight95527.89844
Protein Theoretical pI5.16
Signalling Regions
  • None
Transmembrane Regions
  • 412-432
  • 463-483
  • 541-561
  • 572-592
  • 605-622
  • 636-656
  • 741-761
  • 767-787
Protein Sequence>V-type proton ATPase subunit a, vacuolar isoform MAEKEEAIFRSAEMALVQFYIPQEISRDSAYTLGQLGLVQFRDLNSKVRAFQRTFVNEIR RLDNVERQYRYFYSLLKKHDIKLYEGDTDKYLDGSGELYVPPSGSVIDDYVRNASYLEER LIQMEDATDQIEVQKNDLEQYRFILQSGDEFFLKGDNTDSTSYMDEDMIDANGENIAAAI GASVNYVTGVIARDKVATLEQILWRVLRGNLFFKTVEIEQPVYDVKTREYKHKNAFIVFS HGDLIIKRIRKIAESLDANLYDVDSSNEGRSQQLAKVNKNLSDLYTVLKTTSTTLESELY AIAKELDSWFQDVTREKAIFEILNKSNYDTNRKILIAEGWIPRDELATLQARLGEMIARL GIDVPSIIQVLDTNHTPPTFHRTNKFTAGFQSICDCYGIAQYREINAGLPTIVTFPFMFA IMFGDMGHGFLMTLAALSLVLNEKKINKMKRGEIFDMAFTGRYIILLMGVFSMYTGFLYN DIFSKTMTIFKSGWKWPDHWKKGESITATSVGTYPIGLDWAWHGTENALLFSNSYKMKLS ILMGFIHMTYSYFFSLANHLYFNSMIDIIGNFIPGLLFMQGIFGYLSVCIVYKWAVDWVK DGKPAPGLLNMLINMFLSPGTIDDELYPHQAKVQVFLLLMALVCIPWLLLVKPLHFKFTH KKKSHEPLPSTEADASSEDLEAQQLISAMDADDAEEEEVGSGSHGEDFGDIMIHQVIHTI EFCLNCVSHTASYLRLWALSLAHAQLSSVLWTMTIQIAFGFRGFVGVFMTVALFAMWFAL TCAVLVLMEGTSAMLHSLRLHWVESMSKFFVGEGLPYEPFAFEYKDMEVAVASASSSASS
References
External Links
ResourceLink
Saccharomyces Genome Database VPH1
Uniprot IDP32563
Uniprot NameVPH1_YEAST
GenBank Gene IDM89778
Genebank Protein ID173173
General Reference
  • Manolson, M. F., Proteau, D., Preston, R. A., Stenbit, A., Roberts, B. T., Hoyt, M. A., Preuss, D., Mulholland, J., Botstein, D., Jones, E. W. (1992). "The VPH1 gene encodes a 95-kDa integral membrane polypeptide required for in vivo assembly and activity of the yeast vacuolar H(+)-ATPase." J Biol Chem 267:14294-14303.1385813
  • Cheret, G., Bernardi, A., Sor, F. (1996). "DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae." Yeast 12:1059-1064.8896271
  • Poirey, R., Jauniaux, J. C. (1997). "Sequencing analysis of a 36.8 kb fragment of yeast chromosome XV reveals 26 open reading frames including SEC63, CDC31, SUG2, GCD1, RBL2, PNT1, PAC1 and VPH1." Yeast 13:483-487.9153759
  • Dujon, B., Albermann, K., Aldea, M., Alexandraki, D., Ansorge, W., Arino, J., Benes, V., Bohn, C., Bolotin-Fukuhara, M., Bordonne, R., Boyer, J., Camasses, A., Casamayor, A., Casas, C., Cheret, G., Cziepluch, C., Daignan-Fornier, B., Dang, D. V., de Haan, M., Delius, H., Durand, P., Fairhead, C., Feldmann, H., Gaillon, L., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Nature 387:98-102.9169874
  • Manolson, M. F., Proteau, D., Jones, E. W. (1992). "Evidence for a conserved 95-120 kDa subunit associated with and essential for activity of V-ATPases." J Exp Biol 172:105-112.1491220
  • Leng, X. H., Manolson, M. F., Liu, Q., Forgac, M. (1996). "Site-directed mutagenesis of the 100-kDa subunit (Vph1p) of the yeast vacuolar (H+)-ATPase." J Biol Chem 271:22487-22493.8798414
  • Leng, X. H., Manolson, M. F., Forgac, M. (1998). "Function of the COOH-terminal domain of Vph1p in activity and assembly of the yeast V-ATPase." J Biol Chem 273:6717-6723.9506970
  • Leng, X. H., Nishi, T., Forgac, M. (1999). "Transmembrane topography of the 100-kDa a subunit (Vph1p) of the yeast vacuolar proton-translocating ATPase." J Biol Chem 274:14655-14661.10329659
  • Landolt-Marticorena, C., Kahr, W. H., Zawarinski, P., Correa, J., Manolson, M. F. (1999). "Substrate- and inhibitor-induced conformational changes in the yeast V-ATPase provide evidence for communication between the catalytic and proton-translocating sectors." J Biol Chem 274:26057-26064.10473553
  • Kawasaki-Nishi, S., Nishi, T., Forgac, M. (2001). "Yeast V-ATPase complexes containing different isoforms of the 100-kDa a-subunit differ in coupling efficiency and in vivo dissociation." J Biol Chem 276:17941-17948.11278748
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956