GPI mannosyltransferase 3 (P30777)

Identification
NameGPI mannosyltransferase 3
Synonyms
  • GPI mannosyltransferase III
  • GPI-MT-III
  • Glycosylphosphatidylinositol-anchor biosynthesis protein 10
Gene NameGPI10
Enzyme Class
Biological Properties
General FunctionInvolved in transferase activity, transferring glycosyl groups
Specific FunctionMannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third mannose to Man2-GlcN-acyl-PI during GPI precursor assembly
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG Reactions
6-O-{alpha-D-mannosyl-(1->6)-2-O-[(2-aminoethyl)phosphoryl]-alpha-D-mannosyl-(1->4)-alpha-D-glucosaminyl}-O-acyl-1-phosphatidyl-1D-myo-inositol + dolichyl D-mannosyl phosphate ↔ 6-O-{alpha-D-mannosyl-(1->2)-alpha-D-mannosyl-(1->6)-2-O-[(2-aminoethyl)phosphoryl]-alpha-D-mannosyl-(1->4)-alpha-D-glucosaminyl}-O-acyl-1-phosphatidyl-1D-myo-inositol
Metabolites
YMDB IDNameView
YMDB00937dolichyl D-mannosyl phosphateShow
GO Classification
Component
cell part
membrane part
intrinsic to membrane
intrinsic to organelle membrane
intrinsic to endoplasmic reticulum membrane
Function
transferase activity
transferase activity, transferring glycosyl groups
catalytic activity
Process
metabolic process
macromolecule metabolic process
macromolecule modification
protein modification process
protein amino acid lipidation
GPI anchor biosynthetic process
Gene Properties
Chromosome Locationchromosome 7
LocusYGL142C
Gene Sequence>1851 bp ATGGCTCACGAGGTTCATAGAATAAAACCTAAACTAGGACGGACCCAAATATTTTGGGTT TTTCTAGCCTTTAGAGTACTGAATGCAGTTCTGACGAGAACCTTCTTTCAGGCTGATGAG TTTTGGCAAGCATTGGAGCCGGCACATTGGAAGGCTTTCAAGTATGGTGAACTGACTTGG GAATGGAAATTCGGAGTTCGTAGCTATTTATTTCCGATGATTTTTGAGCTGACTTATAGA TTGGTTTCTCTATCGTCAATCCTTCTTCACTATGCACTTCTTCTTCTCTCAACGATAGGG TCTGATTTATTAATTTTACTACTGCCGAAATATGAGTTAAGTTGGCAAGTCGCTGAAGAC TTGAAGAGGCTTCCTTTTGATGTCACACGGTCGTTCGAATATTATGGTGTGATATATGCG CCAAAAATAGTTATGGCAGTACTAGCTTCCATAGGGGAATATTATATCGTAAGATTTGTT CAAAAGCTCTATTTACTTACACTTGATAAGAGAAACGAGAAGGAGGAAGAGGAGAGACGC AGCGGTTTATCCGAGATTACGAAGTTTGCACTGCTTTTAAGTTTAACTAATTTTTTTAAT TGCTTTTTTATTACAAGAACGTTCATCAACTCTTTTGAAATGATTTTAACTTCTATTGCC CTCTACTACTGGGACTGGACAGGCGGACAAATGATAAAAGAGTCATCTTTTACTAAGTCG CTTATTTTTGCATTTCTGGCATGTTTGCAAAGACCCAGCAGTGGACTTATTTGGGTAATT CCCAGCATTTCCCTAATATTAAACTTGGTAGGCAAAAAGCAGTATCATTTACTTTTCATC ACATTTTCCAAAGTTTTACGGTCTTTTTTTCTGGTATTTACTGCAAATGCAATTATCGAC ATGTATTTTTATGAAAAAGTAACGTTTCCCTTTTTCCGATTTCTAAAATTCAATTTCACA ACACCATTATCAAAGTTTTATGGGGTAGCTCCTTGGCATTTTCATTTCTTTCAAAGCTTA CCTATCGTATTAGGTGCAAGTATTCCCGCTTTTGCATTTGGATTATTTTTTCCACTAAGC AAAAGGAGTTTCCCAAAGAAGTATTTAAACCCGTTTTTCCAAGTGAAACTCACTATCTTG TTGAATCTGTTGGTGTACTCTACGTTACCTCATAAGGAATTTAGGTTCATCTTCCCATTA CAGCCACTTTTCATATTAATATCATCATTTGGCCTTTTGAGACTTGATAGAGATTATTGG AAAAGACTATCTGGCCTAAAATCTTTACTCTGGCTTGTTCCATTCGTGTCGGTGTTCATC GCATTACTTTTGGACACATTCCACGAATCTGGATCTATCGAAGTTATGAAATTTTTACAT GAAGAGCCAGAAATAGATAGTTTGGGATTCATAATGCCCTGTCATTCGACACCTGGTCAA AGCTATTTACACCGCAGTGACATTCAAGATCTGTGGTCGATTACTTGTAACCCTCCATTG CATTTACTTGGAGACCCAGAGGCTTACTCCAAACTAGAGACCTACATGGATGAAAGTGAC CATTTGTATGACGATATATCGGCATTCATTTACAAAAATTTCCCACCGCCTTTTCGTAAG GATCTAAGGAGTCCAGGTAAAACATACAGCCACGAATGGCCAACATATTTGGTTGTTTTC GAGCACATGGAAAACGCATTTCTAAAGGATTTCCTGAAAGATTCATCATACATTGAATAC AATAGATTCTTCAACTCTCTTGCGCACTGGGATTCAAGAAGATCAGGAGACATAATAATT TATTATAAATTACCATTCGATTATAGCGATATTCCAGCGGCAGATATTTAA
Protein Properties
Pfam Domain Function
Protein Residues616
Protein Molecular Weight72564.89844
Protein Theoretical pI8.63
Signalling Regions
  • None
Transmembrane Regions
  • 17-37
  • 87-107
  • 137-157
  • 189-209
  • 241-261
  • 279-299
  • 339-359
  • 393-413
  • 424-444
Protein Sequence>GPI mannosyltransferase 3 MAHEVHRIKPKLGRTQIFWVFLAFRVLNAVLTRTFFQADEFWQALEPAHWKAFKYGELTW EWKFGVRSYLFPMIFELTYRLVSLSSILLHYALLLLSTIGSDLLILLLPKYELSWQVAED LKRLPFDVTRSFEYYGVIYAPKIVMAVLASIGEYYIVRFVQKLYLLTLDKRNEKEEEERR SGLSEITKFALLLSLTNFFNCFFITRTFINSFEMILTSIALYYWDWTGGQMIKESSFTKS LIFAFLACLQRPSSGLIWVIPSISLILNLVGKKQYHLLFITFSKVLRSFFLVFTANAIID MYFYEKVTFPFFRFLKFNFTTPLSKFYGVAPWHFHFFQSLPIVLGASIPAFAFGLFFPLS KRSFPKKYLNPFFQVKLTILLNLLVYSTLPHKEFRFIFPLQPLFILISSFGLLRLDRDYW KRLSGLKSLLWLVPFVSVFIALLLDTFHESGSIEVMKFLHEEPEIDSLGFIMPCHSTPGQ SYLHRSDIQDLWSITCNPPLHLLGDPEAYSKLETYMDESDHLYDDISAFIYKNFPPPFRK DLRSPGKTYSHEWPTYLVVFEHMENAFLKDFLKDSSYIEYNRFFNSLAHWDSRRSGDIII YYKLPFDYSDIPAADI
References
External Links
ResourceLink
Saccharomyces Genome Database GPI10
Uniprot IDP30777
Uniprot NameGPI10_YEAST
GenBank Gene IDX99960
Genebank Protein ID1498242
General Reference
  • Voet, M., Defoor, E., Verhasselt, P., Riles, L., Robben, J., Volckaert, G. (1997). "The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames." Yeast 13:177-182.9046099
  • Tettelin, H., Agostoni Carbone, M. L., Albermann, K., Albers, M., Arroyo, J., Backes, U., Barreiros, T., Bertani, I., Bjourson, A. J., Bruckner, M., Bruschi, C. V., Carignani, G., Castagnoli, L., Cerdan, E., Clemente, M. L., Coblenz, A., Coglievina, M., Coissac, E., Defoor, E., Del Bino, S., Delius, H., Delneri, D., de Wergifosse, P., Dujon, B., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Nature 387:81-84.9169869
  • Pel, H. J., Maat, C., Rep, M., Grivell, L. A. (1992). "The yeast nuclear gene MRF1 encodes a mitochondrial peptide chain release factor and cures several mitochondrial RNA splicing defects." Nucleic Acids Res 20:6339-6346.1475194
  • Sutterlin, C., Escribano, M. V., Gerold, P., Maeda, Y., Mazon, M. J., Kinoshita, T., Schwarz, R. T., Riezman, H. (1998). "Saccharomyces cerevisiae GPI10, the functional homologue of human PIG-B, is required for glycosylphosphatidylinositol-anchor synthesis." Biochem J 332 ( Pt 1):153-159.9576863
  • Canivenc-Gansel, E., Imhof, I., Reggiori, F., Burda, P., Conzelmann, A., Benachour, A. (1998). "GPI anchor biosynthesis in yeast: phosphoethanolamine is attached to the alpha1,4-linked mannose of the complete precursor glycophospholipid." Glycobiology 8:761-770.9639537
  • Imhof, I., Canivenc-Gansel, E., Meyer, U., Conzelmann, A. (2000). "Phosphatidylethanolamine is the donor of the phosphorylethanolamine linked to the alpha1,4-linked mannose of yeast GPI structures." Glycobiology 10:1271-1275.11159918
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258