Protein PBN1 (P25580)

Identification
NameProtein PBN1
Synonyms
  • Protease B non-derepressible protein 1
Gene NamePBN1
Enzyme ClassNot Available
Biological Properties
General FunctionInvolved in ER-associated protein catabolic process
Specific FunctionRequired for proper folding and/or the stability of a subset of proteins in the endoplasmic reticulum. Aids the autocatalytic processing of PRB1. Component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase GPI14
Cellular LocationEndoplasmic reticulum membrane; Single-pass type III membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
GO Classification
Component
cell part
membrane
organelle membrane
endoplasmic reticulum membrane
Process
metabolic process
macromolecule metabolic process
macromolecule modification
protein modification process
protein amino acid lipidation
GPI anchor biosynthetic process
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>1251 bp ATGGTGACAAGACATAGAGTGACTGTACTCTACAATGCCCCTGAGGATATCGGTAATCAT ATGCGCCAAAATGACACTCATTTGACTGTTCGTGGAGGTTCTGGTGTGGTTTTACAACAA AGGTGGCTATTAGAGAGGACTGGAAGCTTGGATAAATCCTTTACGAGAATCACTTGGAGG CCCAGAGCGGACTTGGCTAGAAGTTTAAGCGTTATAGAAAATGAACTGAGTGCTGGCTTT TCAGTTTACTCAAATTCTTCGGATGTGCCGGAAAGGTTTATTACTAACCCAGTCTACAAT TCATTTCACAGTGAGAAGTTTGACATAGAGCAGTACTTGCCTCCCGAAGTAGATTTGAAT CTGTCATGGAATCCAGAAGATTTTACATATGATATATCAGTGGAGCCCACACAAATCCAA ATTGTTGAATATCGTCTGTTGAAACAGGGTGAAGAATTTACAATTGCAAGAGTGAAAGAT GAGAAACTCGAAGTAGGTGTATTCTTTGTGGATGCAAGTGATGAAAGTGATGTCGATATT GGTGGAATACGTTGTAATTGGAGGATGGACGATGGTAAAATGGAAAGATGTCAGAAAACA TCCTTATTGTATAAACAGGGCCATATCGCATACAATCACTCGACGACTACGACATCACTA TATCTGAATGAACCTATCGGTTTGCATCCAAAAATCATGATTGATCTCACAGATTTCGAA GAACGCCCTAAATGCATGTATCTAATGCACCTGCAATTGCCGTTAGAATTATTTATCGAT AAATTCCAATCCTCTCCCTTACTACTTTTTGGAGAAGACGACTTAGAATTACCAGAATAC TCTCTTCGAGATAAGGCATGGGGTTCTGAAAGTATCTTTGAATTGAAAGCCGGCACAATG AATGAAGTGACATTGCATACTAGATATATTGAGCCTTCTAATAATAAAGGGGATAAATTA GAAGTTTCATTTGATCCAGAAGTTATATTAGCCTGCGACACAGGTGACAATAAAGTTTCC CGTAATCCATTTTATAAAAAAGGTCTAGGATATGAATCTCTCTTTACAGACGATACTACA TTCCGCCATTTGAACTCGACAACTCTTCTAGTACCAATTCCAAGGCCTGACACAAAGGAT TATTCCAAGATCAAAAATGGTACGTTACTATGCTTACTCATCTCCATCATATACATTTTC TCCAAGGTATTTGGTAACAACAAGAAGAAAAGATCAGTAAAACGGGAATAA
Protein Properties
Pfam Domain FunctionNot Available
Protein Residues416
Protein Molecular Weight47922.89844
Protein Theoretical pI5.36
Signalling Regions
  • None
Transmembrane Regions
  • 386-405
Protein Sequence>Protein PBN1 MVTRHRVTVLYNAPEDIGNHMRQNDTHLTVRGGSGVVLQQRWLLERTGSLDKSFTRITWR PRADLARSLSVIENELSAGFSVYSNSSDVPERFITNPVYNSFHSEKFDIEQYLPPEVDLN LSWNPEDFTYDISVEPTQIQIVEYRLLKQGEEFTIARVKDEKLEVGVFFVDASDESDVDI GGIRCNWRMDDGKMERCQKTSLLYKQGHIAYNHSTTTTSLYLNEPIGLHPKIMIDLTDFE ERPKCMYLMHLQLPLELFIDKFQSSPLLLFGEDDLELPEYSLRDKAWGSESIFELKAGTM NEVTLHTRYIEPSNNKGDKLEVSFDPEVILACDTGDNKVSRNPFYKKGLGYESLFTDDTT FRHLNSTTLLVPIPRPDTKDYSKIKNGTLLCLLISIIYIFSKVFGNNKKKRSVKRE
References
External Links
ResourceLink
Saccharomyces Genome Database PBN1
Uniprot IDP25580
Uniprot NamePBN1_YEAST
GenBank Gene IDAY692756
Genebank Protein ID51012963
General Reference
  • Oliver, S. G., van der Aart, Q. J., Agostoni-Carbone, M. L., Aigle, M., Alberghina, L., Alexandraki, D., Antoine, G., Anwar, R., Ballesta, J. P., Benit, P., et, a. l. .. (1992). "The complete DNA sequence of yeast chromosome III." Nature 357:38-46.1574125
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Lai, M. H., Silverman, S. J., Gaughran, J. P., Kirsch, D. R. (1997). "Multiple copies of PBS2, MHP1 or LRE1 produce glucanase resistance and other cell wall effects in Saccharomyces cerevisiae." Yeast 13:199-213.9090049
  • Naik, R. R., Jones, E. W. (1998). "The PBN1 gene of Saccharomyces cerevisiae: an essential gene that is required for the post-translational processing of the protease B precursor." Genetics 149:1277-1292.9649520
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Ashida, H., Hong, Y., Murakami, Y., Shishioh, N., Sugimoto, N., Kim, Y. U., Maeda, Y., Kinoshita, T. (2005). "Mammalian PIG-X and yeast Pbn1p are the essential components of glycosylphosphatidylinositol-mannosyltransferase I." Mol Biol Cell 16:1439-1448.15635094
  • Subramanian, S., Woolford, C. A., Drill, E., Lu, M., Jones, E. W. (2006). "Pbn1p: an essential endoplasmic reticulum membrane protein required for protein processing in the endoplasmic reticulum of budding yeast." Proc Natl Acad Sci U S A 103:939-944.16418276