V-type proton ATPase subunit c 2 (P25515)

Identification
NameV-type proton ATPase subunit c 2
Synonyms
  • V-ATPase subunit c
  • Guanine nucleotide exchange factor 2
  • V-ATPase 16 kDa proteolipid subunit 1
  • Vacuolar proton pump c subunit
Gene NameCUP5
Enzyme ClassNot Available
Biological Properties
General FunctionEnergy production and conversion
Specific FunctionProton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. It is an electrogenic proton pump that generates a proton motive force of 180 mv, inside positive and acidic, in the vacuolar membrane vesicles
Cellular LocationVacuole membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
GO Classification
Component
macromolecular complex
protein complex
proton-transporting two-sector ATPase complex, proton-transporting domain
proton-transporting V-type ATPase, V0 domain
Function
transporter activity
transmembrane transporter activity
substrate-specific transmembrane transporter activity
ion transmembrane transporter activity
cation transmembrane transporter activity
inorganic cation transmembrane transporter activity
monovalent inorganic cation transmembrane transporter activity
hydrogen ion transmembrane transporter activity
Process
metabolic process
purine nucleotide biosynthetic process
purine nucleoside triphosphate biosynthetic process
purine ribonucleoside triphosphate biosynthetic process
ATP biosynthetic process
ATP synthesis coupled proton transport
nitrogen compound metabolic process
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleoside phosphate metabolic process
nucleotide metabolic process
purine nucleotide metabolic process
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>483 bp ATGACTGAATTGTGTCCTGTCTACGCCCCTTTCTTTGGTGCCATTGGTTGTGCCTCTGCA ATTATCTTCACCTCATTAGGTGCTGCTTACGGTACTGCTAAGTCTGGTGTTGGTATCTGT GCCACTTGTGTGTTGAGACCAGACCTATTATTCAAGAACATTGTTCCTGTTATTATGGCT GGTATCATTGCCATTTACGGTTTAGTTGTTTCCGTTTTGGTTTGTTATTCGTTGGGTCAA AAGCAAGCTTTGTACACCGGTTTCATCCAATTGGGTGCCGGTCTATCAGTCGGTTTGAGT GGTCTAGCTGCTGGTTTCGCTATTGGTATTGTCGGTGATGCAGGTGTTAGAGGTTCCTCT CAACAACCAAGATTATTCGTCGGTATGATTTTGATTTTGATTTTTGCTGAAGTTTTGGGT CTATACGGTTTGATTGTTGCTTTGTTGTTGAACTCCAGGGCTACTCAAGATGTTGTCTGT TAA
Protein Properties
Pfam Domain Function
Protein Residues160
Protein Molecular Weight16350.40039
Protein Theoretical pI8.1
Signalling Regions
  • None
Transmembrane Regions
  • 9-31
  • 54-74
  • 91-112
  • 125-150
Protein Sequence>V-type proton ATPase subunit c MTELCPVYAPFFGAIGCASAIIFTSLGAAYGTAKSGVGICATCVLRPDLLFKNIVPVIMA GIIAIYGLVVSVLVCYSLGQKQALYTGFIQLGAGLSVGLSGLAAGFAIGIVGDAGVRGSS QQPRLFVGMILILIFAEVLGLYGLIVALLLNSRATQDVVC
References
External Links
ResourceLink
Saccharomyces Genome Database CUP5
Uniprot IDP25515
Uniprot NameVATL1_YEAST
GenBank Gene IDAY558342
Genebank Protein ID45270574
General Reference
  • Nelson, H., Nelson, N. (1989). "The progenitor of ATP synthases was closely related to the current vacuolar H+-ATPase." FEBS Lett 247:147-153.2540044
  • Dietrich, F. S., Mulligan, J., Hennessy, K., Yelton, M. A., Allen, E., Araujo, R., Aviles, E., Berno, A., Brennan, T., Carpenter, J., Chen, E., Cherry, J. M., Chung, E., Duncan, M., Guzman, E., Hartzell, G., Hunicke-Smith, S., Hyman, R. W., Kayser, A., Komp, C., Lashkari, D., Lew, H., Lin, D., Mosedale, D., Davis, R. W., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Nature 387:78-81.9169868
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Noumi, T., Beltran, C., Nelson, H., Nelson, N. (1991). "Mutational analysis of yeast vacuolar H(+)-ATPase." Proc Natl Acad Sci U S A 88:1938-1942.1825730
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258