DNA-3-methyladenine glycosylase (P22134)

Identification
NameDNA-3-methyladenine glycosylase
Synonyms
  • 3-methyladenine DNA glycosidase
  • 3MEA DNA glycosylase
Gene NameMAG1
Enzyme Class
Biological Properties
General FunctionInvolved in alkylbase DNA N-glycosylase activity
Specific FunctionHydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions
Cellular LocationNucleus
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB009867-MethylguanineShow
GO Classification
Component
Not Available
Function
binding
hydrolase activity
hydrolase activity, acting on glycosyl bonds
nucleic acid binding
hydrolase activity, hydrolyzing N-glycosyl compounds
DNA binding
DNA N-glycosylase activity
alkylbase DNA N-glycosylase activity
catalytic activity
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA repair
base-excision repair
metabolic process
macromolecule metabolic process
Gene Properties
Chromosome Locationchromosome 5
LocusYER142C
Gene Sequence>891 bp ATGAAACTAAAAAGGGAGTATGATGAGTTAATAAAAGCAGACGCTGTTAAGGAAATAGCA AAAGAATTAGGGTCTCGACCTCTAGAGGTTGCTCTTCCTGAGAAATATATTGCTAGACAT GAAGAAAAGTTCAATATGGCTTGCGAACACATTTTAGAGAAAGATCCATCACTTTTTCCC ATACTTAAGAATAATGAATTTACGTTGTACTTGAAGGAGACTCAAGTCCCTAATACACTC GAAGATTATTTTATTAGGCTTGCAAGCACAATTTTGTCTCAACAGATCAGTGGCCAAGCA GCTGAAAGCATCAAGGCAAGGGTTGTCAGTCTTTATGGCGGTGCATTTCCTGATTACAAA ATCCTTTTCGAAGACTTCAAAGACCCAGCAAAATGTGCAGAAATCGCAAAATGTGGATTG AGTAAAAGGAAAATGATATATCTAGAGTCTCTTGCTGTCTATTTTACTGAAAAATATAAG GATATCGAAAAGCTCTTCGGTCAAAAAGATAATGATGAGGAAGTGATTGAAAGTTTAGTT ACGAATGTAAAAGGTATAGGCCCATGGAGTGCCAAAATGTTCTTGATCTCCGGATTGAAA AGAATGGATGTATTTGCTCCTGAAGATCTAGGTATTGCTAGGGGTTTTTCAAAATACCTT TCAGATAAGCCAGAATTGGAAAAAGAATTAATGCGTGAAAGAAAAGTAGTTAAAAAGAGT AAGATTAAGCATAAGAAATACAACTGGAAAATATATGACGACGACATAATGGAAAAATGC TCTGAAACATTTTCTCCGTATAGGTCTGTGTTTATGTTCATACTTTGGAGGCTCGCGAGC ACAAATACAGATGCCATGATGAAGGCAGAAGAAAATTTCGTGAAATCCTAA
Protein Properties
Pfam Domain Function
Protein Residues296
Protein Molecular Weight34332.5
Protein Theoretical pI8.26
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>DNA-3-methyladenine glycosylase MKLKREYDELIKADAVKEIAKELGSRPLEVALPEKYIARHEEKFNMACEHILEKDPSLFP ILKNNEFTLYLKETQVPNTLEDYFIRLASTILSQQISGQAAESIKARVVSLYGGAFPDYK ILFEDFKDPAKCAEIAKCGLSKRKMIYLESLAVYFTEKYKDIEKLFGQKDNDEEVIESLV TNVKGIGPWSAKMFLISGLKRMDVFAPEDLGIARGFSKYLSDKPELEKELMRERKVVKKS KIKHKKYNWKIYDDDIMEKCSETFSPYRSVFMFILWRLASTNTDAMMKAEENFVKS
References
External Links
ResourceLink
Saccharomyces Genome Database MAG1
Uniprot IDP22134
Uniprot NameMAG_YEAST
GenBank Gene IDAY692938
Genebank Protein ID51013327
General Reference
  • Berdal, K. G., Bjoras, M., Bjelland, S., Seeberg, E. (1990). "Cloning and expression in Escherichia coli of a gene for an alkylbase DNA glycosylase from Saccharomyces cerevisiae; a homologue to the bacterial alkA gene." EMBO J 9:4563-4568.2265619
  • Chen, J., Derfler, B., Samson, L. (1990). "Saccharomyces cerevisiae 3-methyladenine DNA glycosylase has homology to the AlkA glycosylase of E. coli and is induced in response to DNA alkylation damage." EMBO J 9:4569-4575.2265620
  • Dietrich, F. S., Mulligan, J., Hennessy, K., Yelton, M. A., Allen, E., Araujo, R., Aviles, E., Berno, A., Brennan, T., Carpenter, J., Chen, E., Cherry, J. M., Chung, E., Duncan, M., Guzman, E., Hartzell, G., Hunicke-Smith, S., Hyman, R. W., Kayser, A., Komp, C., Lashkari, D., Lew, H., Lin, D., Mosedale, D., Davis, R. W., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Nature 387:78-81.9169868
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Bjoras, M., Klungland, A., Johansen, R. F., Seeberg, E. (1995). "Purification and properties of the alkylation repair DNA glycosylase encoded the MAG gene from Saccharomyces cerevisiae." Biochemistry 34:4577-4582.7718559
  • Grandier-Vazeille, X., Bathany, K., Chaignepain, S., Camougrand, N., Manon, S., Schmitter, J. M. (2001). "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex." Biochemistry 40:9758-9769.11502169
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956