Ammonia transport outward protein 3 (Q12359)

Identification
NameAmmonia transport outward protein 3
SynonymsNot Available
Gene NameATO3
Enzyme ClassNot Available
Biological Properties
General FunctionInvolved in ammonium transmembrane transporter activity
Specific FunctionTransporter protein required for ammonia export. Induced in rho(0) cells, probably to eliminate the excess ammonia that arises because of a potential defect in ammonia assimilation in those cells
Cellular LocationCell membrane; Multi-pass membrane protein.
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00423AmmoniumShow
GO Classification
Component
membrane
cell part
Function
Not Available
Process
Not Available
Gene Properties
Chromosome Locationchromosome 4
LocusYDR384C
Gene Sequence>828 bp ATGACATCGTCTGCTTCTTCTCCACAGGATTTGGAAAAGGGTGTGAACACTCTAGAAAAT ATTGAGACGCTCCCCCAGCAGGGTTCGATTGCAGGCGTTTCGCAGGGCTTCCCTAATATT CAAGAGATATACTCCGACAGAGACTTCATTACTCTAGGATCCTCCACCTACAGGCGCAGA GATTTGCTCAATGCACTAGACAGAGGGGATGGGGAGGAAGGAAACTGTGCAAAGTATACT CCGCACCAGTTCGCTAATCCGGTTCCTTTGGGTCTCGCATCGTTTTCTCTGTCATGTCTA GTTCTGTCGCTAATTAATGCGAACGTTCGCGGCGTCACTGATGGTAAATGGGCGCTAAGT TTGTTTATGTTTTTTGGTGGTGCCATTGAATTGTTTGCCGGGTTATTGTGTTTTGTCATT GGGGACACATACGCCATGACTGTGTTCAGTTCTTTCGGCGGGTTCTGGATCTGCTACGGT TATGGGCTAACAGATACTGATAATTTGGTCAGCGGCTATACCGATCCCACAATGTTGAAC AATGTCATTGGGTTCTTCCTTGCGGGCTGGACCGTTTTCACCTTCTTGATGTTGATGTGT ACATTGAAGAGCACGTGGGGTTTGTTCTTACTATTGACGTTTTTGGATCTTACCTTCCTA CTACTGTGTATCGGCACCTTCATTGACAACAACAATCTCAAAATGGCCGGTGGCTATTTT GGAATTTTAAGTAGTTGCTGCGGTTGGTATTCGCTATACTGTTCTGTGGTGAGTCCTTCA AATTCTTATTTAGCCTTCAGGGCGCACACAATGCCAAATGCTCCTTAA
Protein Properties
Pfam Domain Function
Protein Residues275
Protein Molecular Weight30028.09961
Protein Theoretical pI4.36
Signalling Regions
  • None
Transmembrane Regions
  • 85-105
  • 121-141
  • 182-202
  • 208-228
  • 237-257
Protein Sequence>Ammonia transport outward protein 3 MTSSASSPQDLEKGVNTLENIETLPQQGSIAGVSQGFPNIQEIYSDRDFITLGSSTYRRR DLLNALDRGDGEEGNCAKYTPHQFANPVPLGLASFSLSCLVLSLINANVRGVTDGKWALS LFMFFGGAIELFAGLLCFVIGDTYAMTVFSSFGGFWICYGYGLTDTDNLVSGYTDPTMLN NVIGFFLAGWTVFTFLMLMCTLKSTWGLFLLLTFLDLTFLLLCIGTFIDNNNLKMAGGYF GILSSCCGWYSLYCSVVSPSNSYLAFRAHTMPNAP
References
External Links
ResourceLink
Saccharomyces Genome Database ATO3
Uniprot IDQ12359
Uniprot NameATO3_YEAST
GenBank Gene IDU28373
Genebank Protein ID849205
General Reference
  • Jacq, C., Alt-Morbe, J., Andre, B., Arnold, W., Bahr, A., Ballesta, J. P., Bargues, M., Baron, L., Becker, A., Biteau, N., Blocker, H., Blugeon, C., Boskovic, J., Brandt, P., Bruckner, M., Buitrago, M. J., Coster, F., Delaveau, T., del Rey, F., Dujon, B., Eide, L. G., Garcia-Cantalejo, J. M., Goffeau, A., Gomez-Peris, A., Zaccaria, P., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature 387:75-78.9169867
  • Palkova, Z., Devaux, F., Icicova, M., Minarikova, L., Le Crom, S., Jacq, C. (2002). "Ammonia pulses and metabolic oscillations guide yeast colony development." Mol Biol Cell 13:3901-3914.12429834
  • Ficarro, S. B., McCleland, M. L., Stukenberg, P. T., Burke, D. J., Ross, M. M., Shabanowitz, J., Hunt, D. F., White, F. M. (2002). "Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Nat Biotechnol 20:301-305.11875433
  • Guaragnella, N., Butow, R. A. (2003). "ATO3 encoding a putative outward ammonium transporter is an RTG-independent retrograde responsive gene regulated by GCN4 and the Ssy1-Ptr3-Ssy5 amino acid sensor system." J Biol Chem 278:45882-45887.12966084
  • Tachibana, C., Yoo, J. Y., Tagne, J. B., Kacherovsky, N., Lee, T. I., Young, E. T. (2005). "Combined global localization analysis and transcriptome data identify genes that are directly coregulated by Adr1 and Cat8." Mol Cell Biol 25:2138-2146.15743812
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
  • Ricicova, M., Kucerova, H., Vachova, L., Palkova, Z. (2007). "Association of putative ammonium exporters Ato with detergent-resistant compartments of plasma membrane during yeast colony development: pH affects Ato1p localisation in patches." Biochim Biophys Acta 1768:1170-1178.17395151
  • Reinders, J., Wagner, K., Zahedi, R. P., Stojanovski, D., Eyrich, B., van der Laan, M., Rehling, P., Sickmann, A., Pfanner, N., Meisinger, C. (2007). "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Mol Cell Proteomics 6:1896-1906.17761666
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956