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Trimethylguanosine synthase (Q12052)

Identification
NameTrimethylguanosine synthase
Synonyms
  • Cap-specific guanine-N2 methyltransferase
  • snRNA/snoRNA cap hypermethylase
Gene NameTGS1
Enzyme Class
Biological Properties
General FunctionInvolved in methyltransferase activity
Specific FunctionCatalyzes the two serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylates the m3G cap on TLC1 telomerase which affects telomere silencing and telomere length regulation. Required for pre-mRNA splicing, pre-rRNA processing and small ribosomal subunit synthesis. Involved in nucleolar structural organization
Cellular LocationNucleus, nucleolus
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00198S-AdenosylhomocysteineShow
YMDB00297S-AdenosylmethionineShow
GO Classification
Component
Not Available
Function
catalytic activity
transferase activity
transferase activity, transferring one-carbon groups
methyltransferase activity
Process
cellular macromolecule metabolic process
RNA metabolic process
RNA modification
RNA processing
RNA methylation
RNA capping
metabolic process
macromolecule metabolic process
Gene Properties
Chromosome Locationchromosome 16
LocusYPL157W
Gene Sequence>948 bp ATGGGAAGGACATTTATTCATGCTTCGAAAATAAAACATGCGGCACGCAAAAGAAAACAT CATTCCAACTTTAGAACTCTGATCAAATTATTGAACAATGATGCCTATAAGATAGAATCA TCAAAACCGTTGAAAAATGGTAAACTTTTCAAATACTGGAAAAATAGGCGTAGATTGTTT TCAAAGATAGATTCGGCGTCGATATATATGACTGATGAGCTGTGGTTCAGTGTGACGCCC GAAAGAATTGCCTGCTTCCTGGCAAATTTTGTTAAGGCATGCATGCCAAATGCCGAAAGA ATACTGGATGTTTTCTGTGGTGGGGGCGGGAACACCATACAATTTGCCATGCAATTTCCT TACGTCTATGGAGTGGACTACAGTATTGAACACATATATTGTACTGCGAAAAACGCCCAA AGCTACGGTGTGGACGACAGAATATGGCTGAAGCGGGGATCCTGGAAAAAGCTAGTCTCT AAGCAGAAACTTTCCAAAATAAAGTACGACTGTGTATTTGGATCACCCCCGTGGGGTGGT CCAGAATACTTAAGAAACGATGTGTATGACTTAGAGCAACATCTAAAGCCTATGGGGATT ACCAAGATGTTGAAAAGTTTTCTTAAACTAAGCCCTAATGTGATTATGTTTTTGCCAAGG AACTCTGATTTGAACCAGCTTTCTCGAGCTACGAGGAAAGTACTAGGTCCATTTGCCAAA TGTAAAGTATTATATGTAAAGGAGAATGGATATATGAAAGGAATATTTTGCATGTGGGGG GAATGCTTTTTTAACTACGAACCAGCAAGTACGGAGAATAGTCGAAGGGAGTCGTCCGAA AAGGAAGAGCTGAGTTCTGAAAACGAAGAGCTCTCGAAAAGAAAAAAGCATGAATCCACA ACAACGACCAAAGATAATACTGTAGACATTTACGACGTAAATGGTTAA
Protein Properties
Pfam Domain Function
Protein Residues315
Protein Molecular Weight36526.80078
Protein Theoretical pI9.99
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Trimethylguanosine synthase MGRTFIHASKIKHAARKRKHHSNFRTLIKLLNNDAYKIESSKPLKNGKLFKYWKNRRRLF SKIDSASIYMTDELWFSVTPERIACFLANFVKACMPNAERILDVFCGGGGNTIQFAMQFP YVYGVDYSIEHIYCTAKNAQSYGVDDRIWLKRGSWKKLVSKQKLSKIKYDCVFGSPPWGG PEYLRNDVYDLEQHLKPMGITKMLKSFLKLSPNVIMFLPRNSDLNQLSRATRKVLGPFAK CKVLYVKENGYMKGIFCMWGECFFNYEPASTENSRRESSEKEELSSENEELSKRKKHEST TTTKDNTVDIYDVNG
References
External Links
ResourceLink
Saccharomyces Genome Database TGS1
Uniprot IDQ12052
Uniprot NameTGS1_YEAST
GenBank Gene IDX96770
Genebank Protein ID1403552
General Reference
  • Purnelle, B., Coster, F., Goffeau, A. (1996). "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators." Yeast 12:1483-1492.8948103
  • Bussey, H., Storms, R. K., Ahmed, A., Albermann, K., Allen, E., Ansorge, W., Araujo, R., Aparicio, A., Barrell, B., Badcock, K., Benes, V., Botstein, D., Bowman, S., Bruckner, M., Carpenter, J., Cherry, J. M., Chung, E., Churcher, C., Coster, F., Davis, K., Davis, R. W., Dietrich, F. S., Delius, H., DiPaolo, T., Hani, J., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Nature 387:103-105.9169875
  • Mouaikel, J., Verheggen, C., Bertrand, E., Tazi, J., Bordonne, R. (2002). "Hypermethylation of the cap structure of both yeast snRNAs and snoRNAs requires a conserved methyltransferase that is localized to the nucleolus." Mol Cell 9:891-901.11983179
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Mouaikel, J., Bujnicki, J. M., Tazi, J., Bordonne, R. (2003). "Sequence-structure-function relationships of Tgs1, the yeast snRNA/snoRNA cap hypermethylase." Nucleic Acids Res 31:4899-4909.12907733
  • Colau, G., Thiry, M., Leduc, V., Bordonne, R., Lafontaine, D. L. (2004). "The small nucle(ol)ar RNA cap trimethyltransferase is required for ribosome synthesis and intact nucleolar morphology." Mol Cell Biol 24:7976-7986.15340060
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Hausmann, S., Zheng, S., Costanzo, M., Brost, R. L., Garcin, D., Boone, C., Shuman, S., Schwer, B. (2008). "Genetic and biochemical analysis of yeast and human cap trimethylguanosine synthase: functional overlap of 2,2,7-trimethylguanosine caps, small nuclear ribonucleoprotein components, pre-mRNA splicing factors, and RNA decay pathways." J Biol Chem 283:31706-31718.18775984
  • Franke, J., Gehlen, J., Ehrenhofer-Murray, A. E. (2008). "Hypermethylation of yeast telomerase RNA by the snRNA and snoRNA methyltransferase Tgs1." J Cell Sci 121:3553-3560.18840651