Lysophospholipase 3 (Q08108)

Identification
NameLysophospholipase 3
Synonyms
  • Phospholipase B 3
Gene NamePLB3
Enzyme Class
Biological Properties
General FunctionInvolved in metabolic process
Specific FunctionSequentially removes both fatty acyl groups from diacylglycerophospholipids and therefore has both phospholipase A and lysophospholipase activities. Substrate preference is phosphatidylserine > phosphatidylinositol. Does not cleave phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid and phosphatidylinositol-bisphosphate
Cellular LocationCell membrane; Lipid-anchor, GPI-anchor (Potential)
SMPDB Pathways
Glycerophospholipid metabolismPW002493 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Glycerophospholipid metabolismec00564 Map00564
SMPDB ReactionsNot Available
KEGG Reactions
hydron + Glycerophosphocholine + fatty acid ↔ 1-O-acylglycerophosphocholine + water
Metabolites
YMDB IDNameView
YMDB00309GlycerophosphocholineShow
YMDB00694sn-Glycero-3-phosphocholineShow
YMDB00862hydronShow
YMDB00890waterShow
GO Classification
Component
Not Available
Function
hydrolase activity, acting on ester bonds
lipase activity
phospholipase activity
catalytic activity
hydrolase activity
Process
organophosphate metabolic process
phospholipid metabolic process
phospholipid catabolic process
metabolic process
Gene Properties
Chromosome Locationchromosome 15
LocusYOL011W
Gene Sequence>2061 bp ATGATACGTCCATTATGTTCAAAAATTATTATCAGTTACATATTCGCAATTTCTCAGTTT CTACTGGCCGCTAATGCGTGGTCGCCCACAGATAGTTATGTTCCTGGCACCGTGTCGTGT CCCGATGACATAAATCTGGTAAGAGAGGCTACGTCTATATCTCAGAATGAGAGCGCATGG TTGGAAAAGAGGAATAAAGTCACTAGTGTAGCTTTAAAAGATTTCTTGACTAGGGCTACT GCAAATTTTTCAGATAGCTCAGAAGTTTTGTCGAAGCTATTTAATGATGGCAACAGCGAA AACCTGCCGAAAATTGCTGTCGCCGTTTCAGGTGGGGGCTATCGGTCCATGCTAACAGGT GCGGGTGTTCTAGCAGCAATGGATAACAGAACTGAAGGTGCTTATGAGCATGGGCTGGGT GGACTTTTACAAAGCACAACATATTTATCTGGTGCCTCGGGCGGCAACTGGCTAGTTGGT ACATTAGCCTTGAACAATTGGACATCCGTGCAAGACATTCTTAATAATATGCAGAACGAC GATTCTATTTGGGATTTGTCAGATTCTATTGTTACCCCCGGCGGCATTAATATATTCAAA ACAGCCAAAAGGTGGGATCATATCTCTAATGCTGTCGAATCTAAGCAGAACGCCGATTAC AATACTTCTTTGGCCGATATTTGGGGCAGAGCCTTGGCGTATAATTTTTTCCCTTCTCTA AATAGAGGGGGTATAGGCCTGACTTGGTCTTCCATTAGGGATTTCCCAGTGTTTCAAAAT GCTGAAATGCCTTTTCCAATTTCTGTTGCGGACGGTAGGTATCCTGGAACAAAAGTCATC AATTTGAACGCAACGGTTTTTGAATTTAATCCCTTTGAAATGGGATCCTGGGATCCCTCT TTGAACTCTTTTGCCAACGTTAAGTACCTTGGAACGAATGTCTCCAATGGTGTACCATTG GAAAGGGGAAAATGTACCGCAGGCTTTGATAATGCAGGTTTTATCATGGGTACTTCCTCC ACCTTATTTAACCAGTTTCTTTTGAGAATAAACTCCACTCACTTACCTAGTTTCATCACA AGATTAGCAAGACATTTTCTAAAGGATTTATCTCAAGATTTTAATGATATCGCTGTTTAC AGTCCTAATCCATTTAAAGACACGAAGTTTTTGGATAGTGACTATACGACAAGTATTGTT GATTCAGACAGCCTATTCTTAGTTGACGGCGGTGAAGACGACGAAAATGTTCCTGTGTTA CCGCTTATACAAAAGGAACGTGATGTGGACATTATTTTTGCGGTAGATAATTCTGCCGAT ATGAGATTGGCTTGGCCTGACGGTTCTTCTTTAGTCCATACCTATGAGCGTCAATTCGTT AAGCAAGGTCAAGGTATGTCCTTCCCATATGTTCCAGATACGAATACATTCGTTAATTTG GGTTTGAACAAGAAACCAACCTTCTTCGGTTGCGATGCTAATAATTTGACCGATCTTCAA TATATTCCACCTCTAGTTGTTTATCTGCCAAATGCAGAATACTCTTTCAATAGTAATCAG AGCGCGTTCAAGCTATCATATTCCGAATCCCAAAGAAGAAGTATGATTCAAAACGGGTTT GAAATTGCAACTAGGAACAACTTCACTGATGATCCTGAATTCATGGGCTGCGTCGGCTGC GCCATCATTAGACGTAAGCAACAAGCTTTGAATATTACTTTGCCTCCGGAATGTGAAACC TGTTTTAAAAATTATTGCTGGAATGGTACCCTGGATACTACCCCATTACCAGATGTTGAA AAGGATGTTCACCACTCCTTTATCAACGTCAATAGCTTCAACAGCTCCATAGGACAAGAA GAAAGTCTTTACGCCGGATCTTCAGCTTCCCAGTCATCGTCTTCATCATCGTCTTCATCT TCGTCTTCGGAAATTCCTTCGGCTACTGCAACCCTTGAGAAAAAGGCAGCTACTAATTCT GGATCACATTTATCTGGCATAAGTGTAAAGTTCTCTGCTATGATTATGCTTACTTTATTG ATGTTTACCGGAGCAGTATAA
Protein Properties
Pfam Domain Function
Protein Residues686
Protein Molecular Weight75076.20313
Protein Theoretical pI4.63
Signalling Regions
  • 1-26
Transmembrane Regions
  • None
Protein Sequence>Lysophospholipase 3 MIRPLCSKIIISYIFAISQFLLAANAWSPTDSYVPGTVSCPDDINLVREATSISQNESAW LEKRNKVTSVALKDFLTRATANFSDSSEVLSKLFNDGNSENLPKIAVAVSGGGYRSMLTG AGVLAAMDNRTEGAYEHGLGGLLQSTTYLSGASGGNWLVGTLALNNWTSVQDILNNMQND DSIWDLSDSIVTPGGINIFKTAKRWDHISNAVESKQNADYNTSLADIWGRALAYNFFPSL NRGGIGLTWSSIRDFPVFQNAEMPFPISVADGRYPGTKVINLNATVFEFNPFEMGSWDPS LNSFANVKYLGTNVSNGVPLERGKCTAGFDNAGFIMGTSSTLFNQFLLRINSTHLPSFIT RLARHFLKDLSQDFNDIAVYSPNPFKDTKFLDSDYTTSIVDSDSLFLVDGGEDDENVPVL PLIQKERDVDIIFAVDNSADMRLAWPDGSSLVHTYERQFVKQGQGMSFPYVPDTNTFVNL GLNKKPTFFGCDANNLTDLQYIPPLVVYLPNAEYSFNSNQSAFKLSYSESQRRSMIQNGF EIATRNNFTDDPEFMGCVGCAIIRRKQQALNITLPPECETCFKNYCWNGTLDTTPLPDVE KDVHHSFINVNSFNSSIGQEESLYAGSSASQSSSSSSSSSSSSEIPSATATLEKKAATNS GSHLSGISVKFSAMIMLTLLMFTGAV
References
External Links
ResourceLink
Saccharomyces Genome Database PLB3
Uniprot IDQ08108
Uniprot NamePLB3_YEAST
GenBank Gene IDZ74753
Genebank Protein ID1419781
General Reference
  • Dujon, B., Albermann, K., Aldea, M., Alexandraki, D., Ansorge, W., Arino, J., Benes, V., Bohn, C., Bolotin-Fukuhara, M., Bordonne, R., Boyer, J., Camasses, A., Casamayor, A., Casas, C., Cheret, G., Cziepluch, C., Daignan-Fornier, B., Dang, D. V., de Haan, M., Delius, H., Durand, P., Fairhead, C., Feldmann, H., Gaillon, L., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Nature 387:98-102.9169874
  • Merkel, O., Fido, M., Mayr, J. A., Pruger, H., Raab, F., Zandonella, G., Kohlwein, S. D., Paltauf, F. (1999). "Characterization and function in vivo of two novel phospholipases B/lysophospholipases from Saccharomyces cerevisiae." J Biol Chem 274:28121-28127.10497163