3,2-trans-enoyl-CoA isomerase (Q05871)

Identification
Name3,2-trans-enoyl-CoA isomerase
Synonyms
  • Delta(3),Delta(2)-enoyl-CoA isomerase
  • D3,D2-enoyl-CoA isomerase
  • Dodecenoyl-CoA isomerase
Gene NameECI1
Enzyme Class
Biological Properties
General FunctionInvolved in catalytic activity
Specific FunctionEssential for the beta oxidation of unsaturated fatty acids
Cellular LocationPeroxisome.
SMPDB Pathways
Fatty acid metabolismPW002460 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Fatty acid metabolismec00071 Map00071
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00215(2E)-Dodecenoyl-CoAShow
YMDB002923Z-dodecenoyl-CoAShow
GO Classification
Component
Not Available
Function
catalytic activity
Process
metabolic process
Gene Properties
Chromosome Locationchromosome 12
LocusYLR284C
Gene Sequence>843 bp ATGTCGCAAGAAATTAGGCAAAATGAGAAAATCAGTTATCGTATTGAAGGACCATTCTTC ATTATTCACTTAATGAACCCTGACAATTTGAATGCACTAGAAGGTGAAGACTATATTTAT TTAGGAGAGTTACTAGAACTAGCGGACAGAAATCGTGATGTATATTTTACAATTATACAA AGCAGTGGTAGATTTTTTTCCAGTGGTGCTGATTTCAAGGGTATTGCAAAAGCCCAAGGG GATGATACCAATAAATATCCTTCGGAAACAAGCAAGTGGGTGTCAAATTTTGTCGCTAGA AATGTTTATGTCACTGATGCCTTCATCAAGCATTCCAAAGTTTTAATTTGCTGTTTGAAT GGACCAGCAATAGGGTTGAGCGCGGCACTGGTAGCGTTATGTGACATTGTGTACAGTATA AATGACAAGGTTTATTTGCTATACCCCTTTGCTAACTTAGGACTAATTACCGAAGGTGGT ACAACGGTCTCTTTGCCATTGAAGTTTGGCACAAATACGACGTATGAATGCCTCATGTTC AACAAACCATTCAAGTACGATATAATGTGCGAGAACGGATTTATAAGCAAGAATTTTAAC ATGCCATCTTCAAACGCTGAAGCGTTCAATGCAAAGGTCTTAGAAGAATTGAGGGAGAAA GTGAAAGGGCTATACCTGCCCAGTTGCTTAGGGATGAAAAAATTGCTGAAATCGAACCAC ATCGATGCATTCAATAAGGCTAACTCAGTGGAAGTAAATGAATCTCTCAAGTATTGGGTA GATGGAGAGCCCTTAAAAAGATTTAGGCAGCTGGGCTCGAAACAAAGGAAGCATCGTTTA TGA
Protein Properties
Pfam Domain Function
Protein Residues280
Protein Molecular Weight31698.09961
Protein Theoretical pI8.33
PDB Fileshow
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>3,2-trans-enoyl-CoA isomerase MSQEIRQNEKISYRIEGPFFIIHLMNPDNLNALEGEDYIYLGELLELADRNRDVYFTIIQ SSGRFFSSGADFKGIAKAQGDDTNKYPSETSKWVSNFVARNVYVTDAFIKHSKVLICCLN GPAIGLSAALVALCDIVYSINDKVYLLYPFANLGLITEGGTTVSLPLKFGTNTTYECLMF NKPFKYDIMCENGFISKNFNMPSSNAEAFNAKVLEELREKVKGLYLPSCLGMKKLLKSNH IDAFNKANSVEVNESLKYWVDGEPLKRFRQLGSKQRKHRL
References
External Links
ResourceLink
Saccharomyces Genome Database ECI1
Uniprot IDQ05871
Uniprot NameECI1_YEAST
GenBank Gene IDAF090442
Genebank Protein ID3643265
PDB ID
1PJH
General Reference
  • Geisbrecht, B. V., Zhu, D., Schulz, K., Nau, K., Morrell, J. C., Geraghty, M., Schulz, H., Erdmann, R., Gould, S. J. (1998). "Molecular characterization of Saccharomyces cerevisiae Delta3, Delta2-enoyl-CoA isomerase." J Biol Chem 273:33184-33191.9837886
  • Johnston, M., Hillier, L., Riles, L., Albermann, K., Andre, B., Ansorge, W., Benes, V., Bruckner, M., Delius, H., Dubois, E., Dusterhoft, A., Entian, K. D., Floeth, M., Goffeau, A., Hebling, U., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hilger, F., Kleine, K., Kotter, P., Louis, E. J., Messenguy, F., Mewes, H. W., Hoheisel, J. D., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Nature 387:87-90.9169871
  • Geisbrecht, B. V., Schulz, K., Nau, K., Geraghty, M. T., Schulz, H., Erdmann, R., Gould, S. J. (1999). "Preliminary characterization of Yor180Cp: identification of a novel peroxisomal protein of saccharomyces cerevisiae involved in fatty acid metabolism." Biochem Biophys Res Commun 260:28-34.10381339
  • Mursula, A. M., van Aalten, D. M., Modis, Y., Hiltunen, J. K., Wierenga, R. K. (2000). "Crystallization and X-ray diffraction analysis of peroxisomal Delta3-Delta2-enoyl-CoA isomerase from Saccharomyces cerevisiae." Acta Crystallogr D Biol Crystallogr 56:1020-1023.10944342
  • Yang, X., Purdue, P. E., Lazarow, P. B. (2001). "Eci1p uses a PTS1 to enter peroxisomes: either its own or that of a partner, Dci1p." Eur J Cell Biol 80:126-138.11302517
  • Mursula, A. M., van Aalten, D. M., Hiltunen, J. K., Wierenga, R. K. (2001). "The crystal structure of delta(3)-delta(2)-enoyl-CoA isomerase." J Mol Biol 309:845-853.11399063
  • Mursula, A. M., Hiltunen, J. K., Wierenga, R. K. (2004). "Structural studies on delta(3)-delta(2)-enoyl-CoA isomerase: the variable mode of assembly of the trimeric disks of the crotonase superfamily." FEBS Lett 557:81-87.14741345