| Identification |
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| Name | Vacuolar protein sorting-associated protein 30 |
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| Synonyms | - Autophagy-related protein 6
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| Gene Name | VPS30 |
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| Enzyme Class | Not Available |
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| Biological Properties |
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| General Function | retrograde transport, endosome to Golgi |
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| Specific Function | Required for cytoplasm to vacuole transport (Cvt), autophagy, nucleophagy, and mitophagy, as a part of the autophagy-specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). Plays also a role in regulation of filamentous growth. |
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| Cellular Location | Not Available |
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| SMPDB Pathways | |
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| KEGG Pathways | Not Available |
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| SMPDB Reactions | |
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| KEGG Reactions | Not Available |
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| Metabolites | | YMDB ID | Name | View |
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| YMDB00109 | Adenosine triphosphate | Show | | YMDB00230 | 1-Phosphatidyl-D-myo-inositol | Show | | YMDB00914 | ADP | Show | | YMDB16165 | 1-Phosphatidyl-1D-myo-inositol 3-phosphate | Show |
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| GO Classification | | Function |
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| pre-autophagosomal structure | | pre-autophagosomal structure membrane | | autophagosome assembly | | autophagy | | cellular response to nitrogen starvation | | CVT pathway | | cytosol | | phosphatidylinositol biosynthetic process | | cytoplasm | | piecemeal microautophagy of nucleus | | endosome membrane | | retrograde transport, endosome to Golgi | | phosphatidylinositol 3-kinase complex, class III, type I | | phosphatidylinositol 3-kinase complex, class III, type II | | late endosome to vacuole transport | | macroautophagy | | pexophagy | | protein targeting to vacuole | | extrinsic component of membrane | | cytoplasmic, membrane-bounded vesicle |
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| Gene Properties |
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| Chromosome Location | Not Available |
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| Locus | |
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| Gene Sequence | >1674 bp
ATGAAGTGCCAAACATGTCACTTACCCCTGCAACTAGACCCATCTTTAGAAGGTCTTAGT
TTAACCCAAAGAAATTTGCTACTTTCAAATAATTCAATTATCACTGCAACGAATGAAAAT
GTCATCAGCAACAAGGGCATCGAAGCCGCAGATAATTGTGGGCCTCAAATACCGAAGGAG
AGGCTGAGGCGATTAGGGGAGATTCAGAACATAAAAGACTTGAATTTAAAAGATGATAAA
TTAATCACTGATTCGTTTGTTTTTCTTAATCACGATGATGATGATAATGCTAATATTACG
AGTAATAGCAGAGAGGACCAGCGTTATGGAAATGCCAACGGAAATGATAATAAAAAAGCT
AATAGTGATACTTCCGATGGAACCAGTACCTTTCGAGATCATGACGAAGAGGAACAAGAA
GCTACGGATGAGGACGAAAACCAACAAATACAATTGAATTCCAAGACTTTGTCCACACAA
GTCAACGCAATGACTAATGTATTTAACATTCTATCTTCTCAAACAAACATAGACTTTCCT
ATCTGTCAGGACTGCTGTAACATCTTGATAAACAGACTAAAAAGTGAGTATGATGATGCA
ATAAAAGAGAGAGATACTTACGCTCAATTTTTATCGAAGTTAGAGAGTCAAAACAAAGAA
ATATCAGAAAGCAATAAAGAAAAGCAGTATTCCCATAATCTTTCGGAAAAAGAAAATTTG
AAAAAGGAAGAGGAAAGACTCTTGGATCAATTATTACGTTTGGAAATGACAGATGATGAC
TTAGATGGAGAACTAGTTCGTTTACAAGAAAAGAAAGTTCAACTGGAAAATGAGAAACTC
CAAAAACTGAGTGATCAGAATTTAATGGATTTAAATAACATTCAATTCAACAAAAATTTA
CAGTCATTAAAACTGCAATACGAATTATCCTTGAATCAATTAGATAAGTTGAGGAAAATC
AATATTTTCAATGCTACATTCAAAATCTCACATAGCGGCCCCTTTGCCACTATAAATGGA
TTGAGATTAGGCAGTATACCAGAATCAGTGGTACCTTGGAAGGAAATCAATGCAGCACTG
GGCCAATTGATTCTTTTACTGGCTACCATAAACAAAAACCTGAAGATAAATTTAGTAGAC
TACGAATTACAACCGATGGGTTCATTTTCCAAAATCAAGAAAAGAATGGTTAACAGTGTC
GAATATAATAATTCCACTACGAACGCTCCCGGTGATTGGTTGATTCTGCCTGTCTACTAT
GATGAGAATTTCAATTTGGGGAGAATCTTCCGCAAGGAAACGAAGTTTGATAAATCATTA
GAAACAACATTAGAAATAATAAGCGAAATCACTCGGCAGCTTTCTACGATAGCGTCATCA
TATTCATCTCAAACACTCACAACAAGCCAAGATGAATCTTCAATGAATAACGCAAATGAC
GTTGAAAATAGTACTTCCATTCTCGAGCTACCCTATATCATGAATAAGGACAAGATTAAT
GGATTATCCGTCAAATTGCATGGTAGCAGTCCCAACCTTGAATGGACAACGGCTATGAAA
TTTTTATTAACCAATGTAAAGTGGTTGTTAGCGTTTTCTTCCAATTTACTATCCAAGTCA
ATTACCTTAAGCCCTACAGTGAATTACAATGATAAGACCATCAGCGGAAACTAA |
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| Protein Properties |
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| Pfam Domain Function | Not Available |
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| Protein Residues | 557 |
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| Protein Molecular Weight | 63260.22 |
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| Protein Theoretical pI | Not Available |
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| PDB File | show |
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| Signalling Regions | Not Available |
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| Transmembrane Regions | Not Available |
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| Protein Sequence | >Vacuolar protein sorting-associated protein 30
MKCQTCHLPLQLDPSLEGLSLTQRNLLLSNNSIITATNENVISNKGIEAADNCGPQIPKE
RLRRLGEIQNIKDLNLKDDKLITDSFVFLNHDDDDNANITSNSREDQRYGNANGNDNKKA
NSDTSDGTSTFRDHDEEEQEATDEDENQQIQLNSKTLSTQVNAMTNVFNILSSQTNIDFP
ICQDCCNILINRLKSEYDDAIKERDTYAQFLSKLESQNKEISESNKEKQYSHNLSEKENL
KKEEERLLDQLLRLEMTDDDLDGELVRLQEKKVQLENEKLQKLSDQNLMDLNNIQFNKNL
QSLKLQYELSLNQLDKLRKINIFNATFKISHSGPFATINGLRLGSIPESVVPWKEINAAL
GQLILLLATINKNLKINLVDYELQPMGSFSKIKKRMVNSVEYNNSTTNAPGDWLILPVYY
DENFNLGRIFRKETKFDKSLETTLEIISEITRQLSTIASSYSSQTLTTSQDESSMNNAND
VENSTSILELPYIMNKDKINGLSVKLHGSSPNLEWTTAMKFLLTNVKWLLAFSSNLLSKS
ITLSPTVNYNDKTISGN |
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| References |
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| External Links | |
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| General Reference | Not Available |
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