Magnesium-activated aldehyde dehydrogenase, cytosolic (P54115)

Identification

Name

Magnesium-activated aldehyde dehydrogenase, cytosolic

Synonyms

Mg(2+)-activated acetaldehyde dehydrogenase
Mg(2+)-ACDH

Gene Name

ALD6

Enzyme Class

1.2.1.3

Biological Properties

General Function

Involved in oxidoreductase activity

Specific Function

An aldehyde + NAD(+) + H(2)O = an acid + NADH

Cellular Location

Cytoplasm

SMPDB Pathways

Fatty acid metabolism	PW002460
Pyruvate metabolism	PW002447
Tryptophan metabolism	PW002442
beta-Alanine metabolism	PW002381
threonine metabolism	PW002401

KEGG Pathways

beta-Alanine metabolism

ec00410

SMPDB Reactions

Acetaldehyde + NAD + Coenzyme A → NADH + hydron + Acetyl-CoA

KEGG Reactions

NADP + Acetaldehyde + water → Acetic acid + NADPH + hydron

Indoleacetaldehyde + NADP + water → NADPH + hydron + indole-3-acetate

Metabolites

YMDB ID	Name	View
YMDB00022	Acetaldehyde	Show
YMDB00045	Coenzyme A	Show
YMDB00056	Acetic acid	Show
YMDB00110	NAD	Show
YMDB00143	NADH	Show
YMDB00312	Acetyl-CoA	Show
YMDB00354	Indoleacetaldehyde	Show
YMDB00426	NADPH	Show
YMDB00427	NADP	Show
YMDB00576	indole-3-acetate	Show
YMDB00862	hydron	Show
YMDB00890	water	Show

GO Classification

Component
Not Available
Function
catalytic activity
oxidoreductase activity
Process
metabolic process
oxidation reduction

Gene Properties

Chromosome Location

chromosome 16

Locus

YPL061W

Gene Sequence

>1503 bp ATGACTAAGCTACACTTTGACACTGCTGAACCAGTCAAGATCACACTTCCAAATGGTTTG ACATACGAGCAACCAACCGGTCTATTCATTAACAACAAGTTTATGAAAGCTCAAGACGGT AAGACCTATCCCGTCGAAGATCCTTCCACTGAAAACACCGTTTGTGAGGTCTCTTCTGCC ACCACTGAAGATGTTGAATATGCTATCGAATGTGCCGACCGTGCTTTCCACGACACTGAA TGGGCTACCCAAGACCCAAGAGAAAGAGGCCGTCTACTAAGTAAGTTGGCTGACGAATTG GAAAGCCAAATTGACTTGGTTTCTTCCATTGAAGCTTTGGACAATGGTAAAACTTTGGCC TTAGCCCGTGGGGATGTTACCATTGCAATCAACTGTCTAAGAGATGCTGCTGCCTATGCC GACAAAGTCAACGGTAGAACAATCAACACCGGTGACGGCTACATGAACTTCACCACCTTA GAGCCAATCGGTGTCTGTGGTCAAATTATTCCATGGAACTTTCCAATAATGATGTTGGCT TGGAAGATCGCCCCAGCATTGGCCATGGGTAACGTCTGTATCTTGAAACCCGCTGCTGTC ACACCTTTAAATGCCCTATACTTTGCTTCTTTATGTAAGAAGGTTGGTATTCCAGCTGGT GTCGTCAACATCGTTCCAGGTCCTGGTAGAACTGTTGGTGCTGCTTTGACCAACGACCCA AGAATCAGAAAGCTGGCTTTTACCGGTTCTACAGAAGTCGGTAAGAGTGTTGCTGTCGAC TCTTCTGAATCTAACTTGAAGAAAATCACTTTGGAACTAGGTGGTAAGTCCGCCCATTTG GTCTTTGACGATGCTAACATTAAGAAGACTTTACCAAATCTAGTAAACGGTATTTTCAAG AACGCTGGTCAAATTTGTTCCTCTGGTTCTAGAATTTACGTTCAAGAAGGTATTTACGAC GAACTATTGGCTGCTTTCAAGGCTTACTTGGAAACCGAAATCAAAGTTGGTAATCCATTT GACAAGGCTAACTTCCAAGGTGCTATCACTAACCGTCAACAATTCGACACAATTATGAAC TACATCGATATCGGTAAGAAAGAAGGCGCCAAGATCTTAACTGGTGGCGAAAAAGTTGGT GACAAGGGTTACTTCATCAGACCAACCGTTTTCTACGATGTTAATGAAGACATGAGAATT GTTAAGGAAGAAATTTTTGGACCAGTTGTCACTGTCGCAAAGTTCAAGACTTTAGAAGAA GGTGTCGAAATGGCTAACAGCTCTGAATTCGGTCTAGGTTCTGGTATCGAAACAGAATCT TTGAGCACAGGTTTGAAGGTGGCCAAGATGTTGAAGGCCGGTACCGTCTGGATCAACACA TACAACGATTTTGACTCCAGAGTTCCATTCGGTGGTGTTAAGCAATCTGGTTACGGTAGA GAAATGGGTGAAGAAGTCTACCATGCATACACTGAAGTAAAAGCTGTCAGAATTAAGTTG TAA

Protein Properties

Pfam Domain Function

Aldedh (PF00171 )

Protein Residues

500

Protein Molecular Weight

54413.69922

Protein Theoretical pI

5.08

Signalling Regions

None

Transmembrane Regions

None

Protein Sequence

>Magnesium-activated aldehyde dehydrogenase, cytosolic MTKLHFDTAEPVKITLPNGLTYEQPTGLFINNKFMKAQDGKTYPVEDPSTENTVCEVSSA TTEDVEYAIECADRAFHDTEWATQDPRERGRLLSKLADELESQIDLVSSIEALDNGKTLA LARGDVTIAINCLRDAAAYADKVNGRTINTGDGYMNFTTLEPIGVCGQIIPWNFPIMMLA WKIAPALAMGNVCILKPAAVTPLNALYFASLCKKVGIPAGVVNIVPGPGRTVGAALTNDP RIRKLAFTGSTEVGKSVAVDSSESNLKKITLELGGKSAHLVFDDANIKKTLPNLVNGIFK NAGQICSSGSRIYVQEGIYDELLAAFKAYLETEIKVGNPFDKANFQGAITNRQQFDTIMN YIDIGKKEGAKILTGGEKVGDKGYFIRPTVFYDVNEDMRIVKEEIFGPVVTVAKFKTLEE GVEMANSSEFGLGSGIETESLSTGLKVAKMLKAGTVWINTYNDFDSRVPFGGVKQSGYGR EMGEEVYHAYTEVKAVRIKL

References

External Links

Resource	Link
Saccharomyces Genome Database	ALD6
Uniprot ID	P54115
Uniprot Name	ALDH6_YEAST
GenBank Gene ID	U39205
Genebank Protein ID	1079681

General Reference

Wang, X., Mann, C. J., Bai, Y., Ni, L., Weiner, H. (1998). "Molecular cloning, characterization, and potential roles of cytosolic and mitochondrial aldehyde dehydrogenases in ethanol metabolism in Saccharomyces cerevisiae." J Bacteriol 180:822-830.9473035
Bussey, H., Storms, R. K., Ahmed, A., Albermann, K., Allen, E., Ansorge, W., Araujo, R., Aparicio, A., Barrell, B., Badcock, K., Benes, V., Botstein, D., Bowman, S., Bruckner, M., Carpenter, J., Cherry, J. M., Chung, E., Churcher, C., Coster, F., Davis, K., Davis, R. W., Dietrich, F. S., Delius, H., DiPaolo, T., Hani, J., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Nature 387:103-105.9169875
Norbeck, J., Blomberg, A. (1997). "Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway." J Biol Chem 272:5544-5554.9038161
Meaden, P. G., Dickinson, F. M., Mifsud, A., Tessier, W., Westwater, J., Bussey, H., Midgley, M. (1997). "The ALD6 gene of Saccharomyces cerevisiae encodes a cytosolic, Mg(2+)-activated acetaldehyde dehydrogenase." Yeast 13:1319-1327.9392076
Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
Peng, J., Schwartz, D., Elias, J. E., Thoreen, C. C., Cheng, D., Marsischky, G., Roelofs, J., Finley, D., Gygi, S. P. (2003). "A proteomics approach to understanding protein ubiquitination." Nat Biotechnol 21:921-926.12872131
Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956