Ammonium transporter MEP3 (P53390)

Identification
NameAmmonium transporter MEP3
SynonymsNot Available
Gene NameMEP3
Enzyme ClassNot Available
Biological Properties
General FunctionInvolved in ammonium transmembrane transporter activity
Specific FunctionTransporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. The affinity of MEP2 is about twenty times higher than that of MEP1. MEP3 has the lowest affinity
Cellular LocationMembrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00423AmmoniumShow
GO Classification
Component
cell part
membrane
Function
cation transmembrane transporter activity
organic cation transmembrane transporter activity
ammonium transmembrane transporter activity
transporter activity
transmembrane transporter activity
substrate-specific transmembrane transporter activity
ion transmembrane transporter activity
Process
transmembrane transport
establishment of localization
transport
Gene Properties
Chromosome Locationchromosome 16
LocusYPR138C
Gene Sequence>1470 bp ATGGCTCGGGGTGACGGCCCTCTATGGACAGAGACATATGATAGTTCCACAGTCGCTTTT ATGATTTTAGGTGCCGCCCTGGTTTTCTTCATGGTACCGGGGCTGGGCTTTCTTTATTCC GGTTTAGCAAGAAGAAAATCTGCTCTGGCTTTGATTTGGGTAGTGATAATGGCTACCTTA GTAGGTATACTGCAATGGTATTTTTGGGGCTATTCTTTAGCATTCTCTAAGACTGCGACG AACAACAAATTTATCGGCAACTTGGATTCATTTGGGTTTAGAAACGTCTATGGCAAAATT TCGGATGATTCCACGTATCCTGAACTGATTTATGCCATTTTCCAAATGATGTTCATGTGT GTCGCATTGAGTATTATAGCTGGTGCCACTGCGGAAAGAGGTAAGCTTTTTCCACATATG GTTTTTCTTTTTGTTTTTGCGACTTTGGTTTACTGTCCCATCACTTATTGGATTTGGGCC CCAGGTGGTTGGGCCTACCAATGGGGGGTATTAGACTGGGCTGGCGGTGGGAATATTGAA ATCCTAAGTGCTGTGGCTGGTTTCGTTTATTCTTATTTTCTAGGAAGAAGAAAAGAAAAC CTCCTGATCAACTTTAGACCACATAATGTTTCCATGGTGACTTTGGGTACTTCTATACTT TGGTTTGGTTGGTTGCTTTTCAATGCTGCAAGCTCACTGTCACCAAATATGAGGTCCGTA TATGCGTTCATGAACACTTGTCTCAGCGCCACCACGGGTGGAATGACGTGGTGTTTATTA GATTATCGATCTGAAAAAAAATGGTCCACTGTTGGGTTATGCTCCGGCATTATCTGTGGT TTAGTTGCTGCCACGCCTAGCTCGGGTTGTATTACTCTATATGGCTCTTTGATCCAAGGT ATAATAGCGGGTGTTGTTTGTAATTTTGCAACAAAAATAAAGTATTATTTAAAAGTGGAT GATTCCTTAGATCTATTAGCTGAACACGGTATCGCCGGTGTGGTGGGATTGATTTTTAAC GCTCTATTTGCAGCTGATTGGGTTATTGGAATGGACGGCACAACAAAGCATAAGGGTGGT TGGTTGACGCATAACTGGAAACAAATGTATATTCAAATTGCCTATATCGGTGCCTCTGCC GGCTATTGTGCTGTGGTCACGGCCATCATTTGCTTCGTATTAGGTAAAATTCCGGGTGTC CATCTAAGAGTCACTGAGGAAGCCGAAGCATTGGGGTTGGATGAAGATCAAATAGGCGAA TTCGCTTACGATTACGTGGAGGTTAGGAGAGATTATTACCAGTGGGGTGTAGATACAGAT GCACTTCATACTACATGCAATGGCGCTAATTCTGCGTCTGAGACAAATCCTACTGAGGAC AGCCAAAACTCCTCATTGTCATCAGCTACAGTAAGCGGCCAAAACGAAAAAAGTAATAAT CCTAAATTGCATCACGCAAAAGAAGCATGA
Protein Properties
Pfam Domain Function
Protein Residues489
Protein Molecular Weight53689.39844
Protein Theoretical pI6.5
Signalling Regions
  • None
Transmembrane Regions
  • 18-38
  • 49-69
  • 109-129
  • 140-160
  • 174-194
  • 210-230
  • 240-260
  • 268-288
  • 290-310
  • 331-351
  • 373-393
Protein Sequence>Ammonium transporter MEP3 MARGDGHLWTETYDSSTVAFMILGAALVFFMVPGLGFLYSGLARRKSALALIWVVIMATL VGILQWYFWGYSLAFSKTATNNKFIGNLDSFGFRNVYGKISDDSTYPELIYAIFQMMFMC VALSIIAGATAERGKLFPHMVFLFVFATLVYCPITYWIWAPGGWAYQWGVLDWAGGGNIE ILSAVAGFVYSYFLGRRKENLLINFRPHNVSMVTLGTSILWFGWLLFNAASSLSPNMRSV YAFMNTCLSATTGGMTWCLLDYRSEKKWSTVGLCSGIICGLVAATPSSGCITLYGSLIQG IIAGVVCNFATKIKYYLKVDDSLDLLAEHGIAGVVGLIFNALFAADWVIGMDGTTKHKGG WLTHNWKQMYIQIAYIGASAGYCAVVTAIICFVLGKIPGVHLRVTEEAEALGLDEDQIGE FAYDYVEVRRDYYQWGVDTDALHTTCNGANSASETNPTEDSQNSSLSSATVSGQNEKSNN PKLHHAKEA
References
External Links
ResourceLink
Saccharomyces Genome Database MEP3
Uniprot IDP53390
Uniprot NameMEP3_YEAST
GenBank Gene IDAY692775
Genebank Protein ID51013001
General Reference
  • Marini, A. M., Soussi-Boudekou, S., Vissers, S., Andre, B. (1997). "A family of ammonium transporters in Saccharomyces cerevisiae." Mol Cell Biol 17:4282-4293.9234685
  • Bussey, H., Storms, R. K., Ahmed, A., Albermann, K., Allen, E., Ansorge, W., Araujo, R., Aparicio, A., Barrell, B., Badcock, K., Benes, V., Botstein, D., Bowman, S., Bruckner, M., Carpenter, J., Cherry, J. M., Chung, E., Churcher, C., Coster, F., Davis, K., Davis, R. W., Dietrich, F. S., Delius, H., DiPaolo, T., Hani, J., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Nature 387:103-105.9169875
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Soupene, E., Ramirez, R. M., Kustu, S. (2001). "Evidence that fungal MEP proteins mediate diffusion of the uncharged species NH(3) across the cytoplasmic membrane." Mol Cell Biol 21:5733-5741.11486013
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258