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Identification
NameInositol-1,4,5-trisphosphate 5-phosphatase 2
Synonyms
  • Synaptojanin-like protein 2
Gene NameINP52
Enzyme Class
Biological Properties
General FunctionInvolved in inositol or phosphatidylinositol phosphatase activity
Specific FunctionControls the cellular levels and subcellular distribution of phosphatidylinositol 3-phosphate and phosphatidylinositol 4,5-bisphosphate. Specifically functions within the early endocytic pathway and actin organization
Cellular LocationCytoplasm, cytoskeleton, actin patch
SMPDB Pathways
Inositol MetabolismPW002498 ThumbThumb?image type=greyscaleThumb?image type=simple
Inositol phosphate metabolismPW002495 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Inositol phosphate metabolismec00562 Map00562
SMPDB Reactions
1-phosphatidyl-1D-myo-inositol 4-phosphate + Adenosine triphosphate1-phosphatidyl-1D-myo-inositol 4,5-biphosphate + ADP
KEGG Reactions
water + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate ↔ phosphate + 1-phosphatidyl-1D-myo-inositol 3-phosphate + hydron
1-phosphatidyl-1D-myo-inositol 3-phosphate + waterphosphate + 1-Phosphatidyl-D-myo-inositol + hydron
water + 1D-myo-inositol 4,5-bisphosphatephosphate + 1-phosphatidyl-1D-myo-inositol 4-phosphate + hydron
water + 1D-myo-inositol 4,5-bisphosphatephosphate + 1-phosphatidyl-1D-myo-inositol 4-phosphate + hydron
1-phosphatidyl-1D-myo-inositol 4-phosphate + waterphosphate + 1-Phosphatidyl-D-myo-inositol + hydron
Metabolites
YMDB IDNameView
YMDB00109Adenosine triphosphateShow
YMDB00291Inositol 1,4,5-trisphosphateShow
YMDB008521D-myo-inositol 1,4-bisphosphateShow
YMDB00862hydronShow
YMDB00890waterShow
YMDB00907phosphateShow
YMDB00914ADPShow
YMDB162491-phosphatidyl-1D-myo-inositol 4-phosphate Show
YMDB162511-phosphatidyl-1D-myo-inositol 4,5-biphosphateShow
GO Classification
Component
Not Available
Function
phosphatase activity
inositol or phosphatidylinositol phosphatase activity
catalytic activity
hydrolase activity
hydrolase activity, acting on ester bonds
phosphoric ester hydrolase activity
Process
Not Available
Gene Properties
Chromosome Locationchromosome 14
LocusYNL106C
Gene Sequence>3552 bp ATGAAAATACTCCTATCGAAACAGCAAACTAGAAAGATAGCAATAGTTTCAGAAACACAT GGACTGGTATTTAGGCCAATAAACAGTAAGAACTCTCGCAGATCTACATGTGCCGTGGAA TTGGTTCCGAAAGCTGAATTAAATGGTAATGGTTTCAGAAGACTATCTAACCATGAAATA TACGGTTTTATTGGTCTCATAGAAATTGAAGGCCTTATGTTCATAGCAACCATAACAGGT AAATCTAAGGTGGCCCAGCCGATCCCAAACAAAACGGTTAACAAAATATATGCTGTGGAT TTTTTTTGCTTAAATAATTCTAAATGGGATTTCATGGACATCGATTCATCCGGTTATCCG ATTGTTACTAATGATGGAGATTTTGCAATAAGCTCGCCTCCTAGTATTTCCACACATTCT TCTAGATCATCGCTACGTTCTTCTTCCTCTAGGAGTTTGAATGCTCAAGAACAAGCTCCC AAGCATCCATGTCATGAACTAAGAAAGCTATTATCAAATGGATCTTTTTATTACAGCACC GATTTTGATTTAACTTGTACATTGCAGAAACGTGGATTCACGGAACACTCTTTGAGTTTT GATGATTTTGACCGGGAGTTTATGTGGAACTCGTTTCTTATGGACGAAATCATCACATAC AGAGACAGACTAGATGTAACGGCCAAAGAACTATTGGATCAACGAGGATTCCTCACGACC GTAATCCGAGGGTTTGCTGAAACAATTTTTAGCTATATTAATAGGTTGAAAGTAGGACTT ACCATTATTTCCAGGCAAAGTTGGAAAAGAGCAGGCACAAGGTTTAATGCACGTGGTATA GATGATGATGGCCATGTCGCAAATTTTGTTGAAACAGAAATGATTATGTACTCTTCTCAG TATTGTTATGCTTTCACGCAGATAAGAGGAAGCCTTCCAATTTTTTGGGAGCAGGATACA TCTTTGATTAGTCCAAAGATTCAAATTACCAGATCTGTTGAAGCAACACAGCCCACGTTT GATGAACATTTTATAAGACTATTTAAGAAGTATGGGCCAGTTCATATAATCAACCTGCTG TCTACTAAATCTTCAGAAATTCAATTATCGAGGCGATACAAGGAACAGTTGAAAAATTCT GAGAAAATGAAAATCGGTAGAGATGTGTTCCTAACAAGCTTTGATTTCCATAGAGAAACT TCGCAAGATGGGTTTGCAGCTGCAAGTAGGATTATCCCTAAAATAAGAAACACAATTTTG GACGCCGGTTACTTTTCATACGATGTAAAAGAAGGGCGATTAATATCCGAACAAGACGGT GTCTTTAGAACCAATTGCTTGGATTGTTTAGATAGAACAAATTTGATCCAACAGACGATT TCACTGGCCGTTTTCAAACTATTTTTGGAAGACTTCCGATTGGTAAAACCCAGTAGTTTC ATCGACGATAATGAATTTGTGCAAAAGGTGAATGCTTTATGGGCCGACAACGGAGATCAG ATATCACAAATTTACACTGGGACAAATGCTCTGAAATCGTCCTATTCAAGGAAAGGTAAA ATGTCTTTTTCTGGCGCCCTTTCTGATGCGACGAAGTCCGTTAGTAGAATGTACATCAAC AATTTTGTAGATAAAGGAAAACAGCAAAATATTGACACACTTTTGGGCAAACTACCGCAC CAGCAAGTTGTTGAACTTTATGATCCAATCTGCGAATACGTCAATGAAAGGCTGTTAGAG TCAGAGGAAAAATTCACCACTCATTCTAACATAAATTTGTTTGTCGGTACATTCAATGTT AACGGAAACTCTCGAAGAGCAGATTTGTCAAAATGGTTATTTCCAATCGGCGATAAATTC AAACCTGATGTCGTTGTGTTAGGGTTGCAGGAAGTTATAGAGTTGACCGCAGGGTCTATC TTGAATGCAGATTATACGAAAAGTTCTTTTTGGGAAACTATGGTTACTGACTGTTTGAAT CAATACGAAGAAAAATATCTTTTACTTAGAGTGGAACAGATGTCCTCTCTTTTGATACTA TTTTTTGCAAGATCAGATAGAGCCTATAACATAAAAGAGGTAGGTGGGTCTACCAAAAAG ACTGGGTTTGGTGGTATTACTGGAAACAAAGGTGCAGTAGCCATTAGATTCGATTATGGG GCAACTTCATTCTGTTTCGTTAATACACATTTATCAGCAGGTGCGAGTAACATTGACGAA CGTCGCAATGACTACAACAATATCTACAGGAACATAACCTTTCCAAGATCAAAGACAATT CCACACCATGATTCGTTGTTTTGGTTGGGTGACCTTAACTACAGGATAACTTTGACAAAT GATGAAGTTAGAAGAGAATTAAGGGCTCAAAAGGATGGCTATATTGATAGATTGCTTCAA TATGATCAGTTGACTCAAGAAATAAATGAAGGAGTGGTATTTCAGGGCTTTAAAGAACCG ACCCTCCAATTCCGTCCGACCTATAAATATGATTATGGCACAGATAATTATGATACTTCT GAAAAGGCGAGAACTCCGTCTTGGACTGATAGAATCATATATAAAGGTGAAAACCTGCAT CCATTGGCATATTCGGACGCACCTTTAAAAATAAGCGATCATAAACCAGTTTATGCTGCA TATAGGGCAAATGTGAAATTTGTTGACGAAAAAGAGAAGCTTAACCTAGTTGAGAAGCTG TATGCAGAATATAAGAATACGCATCCCGAAGCACTAACTACAGGTCCTGACGAACTTTCT CATGCAAGAATGGAAAAACAAAAGGAAAGTATACCCTTAGATGCAACCGTTCAATCAGCG GGCATAAAACTAATTGACCTAGATGATACTTCCTCGTGCGTTTCGCCTCTACTTTCTGGT CCCAGTCCCCAACCGTCTGTTGTTGGCCCTGGAGGACTCAGTAACGTATCACCCGATAAA AGTAAGCTCAATGTGCTTCCTCCGCCTCCTCCTACCTCGAGGCACAATAAGGAACCATCT TCTAAACTTCTTTCCCCTACCAAAGAGATTTCCATTGTCAGCGTATCGCCGCGTAAAGGT GAGAGCAATCTTCCTGCCTTAGAGAGACACAGTACACCCAAACCACTGCCGCCAGTGCCC GCTTTATCTTTATCTAAGCCAGTCTCTTTACAGAAAAGTTCTTCAGAATTACAGCATGCT AAAGAGACCATAGATAATGGGAAAATTGTTCCTCGACCTTGTCCTCCTATTAGAAGGAAG AGCAGTACTGCCCCGGACGAAATCTCTACATCTACCAAAAATTCAGGTGTGTCCACTACA GAAGATCCTGAGCCAGCAAAGGCCTCCACAAAACCTGAGAAGCCACCAGTTGTCAAAAAA CCGCATTATTTAAGTGTTGCTGCTAACAAGCTTAACACCTCTCAAGAACACTCAATAAAA GTTTCCCCGTCGAATTCAAAATCTGAAGAGGAACTCCCTTGCAAGAAAAAGTCTAAACCA AAAGTTCCTGCGAAGAACCCTGAACTAGAGAAGCTAAGCGTTCATCCATTGAAGCCTTGC GACCCCAATTAA
Protein Properties
Pfam Domain Function
Protein Residues1183
Protein Molecular Weight133330.0
Protein Theoretical pI8.89
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Inositol-1,4,5-trisphosphate 5-phosphatase 2 MKILLSKQQTRKIAIVSETHGLVFRPINSKNSRRSTCAVELVPKAELNGNGFRRLSNHEI YGFIGLIEIEGLMFIATITGKSKVAQPIPNKTVNKIYAVDFFCLNNSKWDFMDIDSSGYP IVTNDGDFAISSPPSISTHSSRSSLRSSSSRSLNAQEQAPKHPCHELRKLLSNGSFYYST DFDLTCTLQKRGFTEHSLSFDDFDREFMWNSFLMDEIITYRDRLDVTAKELLDQRGFLTT VIRGFAETIFSYINRLKVGLTIISRQSWKRAGTRFNARGIDDDGHVANFVETEMIMYSSQ YCYAFTQIRGSLPIFWEQDTSLISPKIQITRSVEATQPTFDEHFIRLFKKYGPVHIINLL STKSSEIQLSRRYKEQLKNSEKMKIGRDVFLTSFDFHRETSQDGFAAASRIIPKIRNTIL DAGYFSYDVKEGRLISEQDGVFRTNCLDCLDRTNLIQQTISLAVFKLFLEDFRLVKPSSF IDDNEFVQKVNALWADNGDQISQIYTGTNALKSSYSRKGKMSFSGALSDATKSVSRMYIN NFVDKGKQQNIDTLLGKLPHQQVVELYDPICEYVNERLLESEEKFTTHSNINLFVGTFNV NGNSRRADLSKWLFPIGDKFKPDVVVLGLQEVIELTAGSILNADYTKSSFWETMVTDCLN QYEEKYLLLRVEQMSSLLILFFARSDRAYNIKEVGGSTKKTGFGGITGNKGAVAIRFDYG ATSFCFVNTHLSAGASNIDERRNDYNNIYRNITFPRSKTIPHHDSLFWLGDLNYRITLTN DEVRRELRAQKDGYIDRLLQYDQLTQEINEGVVFQGFKEPTLQFRPTYKYDYGTDNYDTS EKARTPSWTDRIIYKGENLHPLAYSDAPLKISDHKPVYAAYRANVKFVDEKEKLNLVEKL YAEYKNTHPEALTTGPDELSHARMEKQKESIPLDATVQSAGIKLIDLDDTSSCVSPLLSG PSPQPSVVGPGGLSNVSPDKSKLNVLPPPPPTSRHNKEPSSKLLSPTKEISIVSVSPRKG ESNLPALERHSTPKPLPPVPALSLSKPVSLQKSSSELQHAKETIDNGKIVPRPCPPIRRK SSTAPDEISTSTKNSGVSTTEDPEPAKASTKPEKPPVVKKPHYLSVAANKLNTSQEHSIK VSPSNSKSEEELPCKKKSKPKVPAKNPELEKLSVHPLKPCDPN
References
External Links
ResourceLink
Saccharomyces Genome Database INP52
Uniprot IDP50942
Uniprot NameINP52_YEAST
GenBank Gene IDZ71382
Genebank Protein ID1302022
General Reference
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  • Ooms, L. M., McColl, B. K., Wiradjaja, F., Wijayaratnam, A. P., Gleeson, P., Gething, M. J., Sambrook, J., Mitchell, C. A. (2000). "The yeast inositol polyphosphate 5-phosphatases inp52p and inp53p translocate to actin patches following hyperosmotic stress: mechanism for regulating phosphatidylinositol 4,5-bisphosphate at plasma membrane invaginations." Mol Cell Biol 20:9376-9390.11094088
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  • Wicky, S., Frischmuth, S., Singer-Kruger, B. (2003). "Bsp1p/Ypr171p is an adapter that directly links some synaptojanin family members to the cortical actin cytoskeleton in yeast." FEBS Lett 537:35-41.12606027
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956
  • Stolz, L. E., Huynh, C. V., Thorner, J., York, J. D. (1998). "Identification and characterization of an essential family of inositol polyphosphate 5-phosphatases (INP51, INP52 and INP53 gene products) in the yeast Saccharomyces cerevisiae." Genetics 148:1715-1729.9560389
  • Bottcher, C., Wicky, S., Schwarz, H., Singer-Kruger, B. (2006). "Sjl2p is specifically involved in early steps of endocytosis intimately linked to actin dynamics via the Ark1p/Prk1p kinases." FEBS Lett 580:633-641.16406366
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