Bis(5'-adenosyl)-triphosphatase (P49775)

Identification
NameBis(5'-adenosyl)-triphosphatase
Synonyms
  • AP3A hydrolase
  • AP3Aase
  • Diadenosine 5',5'''-P1,P3-triphosphate hydrolase
  • Dinucleosidetriphosphatase
  • Hit family protein 2
Gene NameHNT2
Enzyme Class
Biological Properties
General FunctionInvolved in catalytic activity
Specific FunctionCleaves A-5'-PPP-5'A to yield AMP and ADP. Can cleave all dinucleoside polyphosphates, provided the phosphate chain contains at least 3 phosphates and that 1 of the 2 bases composing the nucleotide is a purine. Is most effective on dinucleoside triphosphates. Negatively regulates intracellular dinucleoside polyphosphate levels, which elevate following heat shock
Cellular LocationCytoplasm. Nucleus. Mitochondrion
SMPDB PathwaysNot Available
KEGG Pathways
Purine metabolismec00230 Map00230
SMPDB ReactionsNot Available
KEGG Reactions
P1,P4-Bis(5'-adenosyl) tetraphosphate + water ↔ hydron + ADP
Metabolites
YMDB IDNameView
YMDB00097Adenosine monophosphateShow
YMDB00134Diadenosine triphosphateShow
YMDB00590P(1),P(4)-bis(5'-adenosyl) tetraphosphateShow
YMDB00862hydronShow
YMDB00890waterShow
YMDB00914ADPShow
GO Classification
Component
Not Available
Function
catalytic activity
Process
Not Available
Gene Properties
Chromosome Locationchromosome 4
LocusYDR305C
Gene Sequence>654 bp ATGATCTTAAGCAAAACAAAGAAACCTAAAAGCATGAATAAGCCAATATATTTCAGCAAG TTTCTTGTAACTGAACAAGTTTTCTATAAGTCAAAATATACGTATGCATTGGTAAACTTG AAACCAATTGTACCCGGACATGTACTGATCGTTCCACTGAGAACTACGGTTTTAAACTTA TCAGATCTGACCATGCCTGAATCTCAAGATTATTTCAAGACTTTACAGTTAATACATAGA TTTATTAAGTGGCAATATAAGGCTGACTCTATCAACGTTGCCATCCAAGATGGACCTGAG GCCGGACAGTCGGTACCCCACTTGCATACACACATTATTCCACGTTATAAAATAAACAAT GTAGGGGATTTAATATACGATAAATTAGATCATTGGGATGGTAATGGGACTTTGACTGAT TGGCAAGGAAGAAGAGACGAATATTTGGGTGTTGGTGGTAGGCAAGCAAGAAAAAATAAC TCTACATCGGCCACTGTAGACGGGGATGAACTCTCACAAGGTCCTAATGTCTTGAAGCCA GACAGTCAAAGGAAGGTTCGAGCTTTAACCGAAATGAAAAAAGAAGCTGAGGATCTTCAA GCCAGACTAGAAGAATTTGTATCTTCTGATCCTGGTTTAACACAATGGTTATAG
Protein Properties
Pfam Domain Function
Protein Residues206
Protein Molecular Weight23541.59961
Protein Theoretical pI7.9
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Bis(5'-adenosyl)-triphosphatase MNKPIYFSKFLVTEQVFYKSKYTYALVNLKPIVPGHVLIVPLRTTVLNLSDLTMPESQDY FKTLQLIHRFIKWQYKADSINVAIQDGPEAGQSVPHLHTHIIPRYKINNVGDLIYDKLDH WDGNGTLTDWQGRRDEYLGVGGRQARKNNSTSATVDGDELSQGPNVLKPDSQRKVRALTE MKKEAEDLQARLEEFVSSDPGLTQWL
References
External Links
ResourceLink
Saccharomyces Genome Database HNT2
Uniprot IDP49775
Uniprot NameHNT2_YEAST
GenBank Gene IDU28374
Genebank Protein ID849222
General Reference
  • Jacq, C., Alt-Morbe, J., Andre, B., Arnold, W., Bahr, A., Ballesta, J. P., Bargues, M., Baron, L., Becker, A., Biteau, N., Blocker, H., Blugeon, C., Boskovic, J., Brandt, P., Bruckner, M., Buitrago, M. J., Coster, F., Delaveau, T., del Rey, F., Dujon, B., Eide, L. G., Garcia-Cantalejo, J. M., Goffeau, A., Gomez-Peris, A., Zaccaria, P., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature 387:75-78.9169867
  • Brevet, A., Chen, J., Fromant, M., Blanquet, S., Plateau, P. (1991). "Isolation and characterization of a dinucleoside triphosphatase from Saccharomyces cerevisiae." J Bacteriol 173:5275-5279.1653209
  • Chen, J., Brevet, A., Blanquet, S., Plateau, P. (1998). "Control of 5',5'-dinucleoside triphosphate catabolism by APH1, a Saccharomyces cerevisiae analog of human FHIT." J Bacteriol 180:2345-2349.9573184
  • Rubio-Texeira, M., Varnum, J. M., Bieganowski, P., Brenner, C. (2002). "Control of dinucleoside polyphosphates by the FHIT-homologous HNT2 gene, adenine biosynthesis and heat shock in Saccharomyces cerevisiae." BMC Mol Biol 3:7.12028594
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Reinders, J., Zahedi, R. P., Pfanner, N., Meisinger, C., Sickmann, A. (2006). "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." J Proteome Res 5:1543-1554.16823961