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Identification
NamePhosphatidylinositol N-acetylglucosaminyltransferase GPI2 subunit
SynonymsNot Available
Gene NameGPI2
Enzyme Class
Biological Properties
General FunctionInvolved in phosphatidylinositol N-acetylglucosaminyltransferase activity
Specific FunctionPart of the complex catalyzing the transfer of N- acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis
Cellular LocationMembrane; Multi-pass membrane protein (Potential)
SMPDB PathwaysNot Available
KEGG Pathways
Glycosylphosphatidylinositol(GPI)-anchor biosynthesisec00563 Map00563
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB002301-Phosphatidyl-D-myo-inositolShow
YMDB00277Uridine diphosphate-N-acetylglucosamineShow
YMDB00307Uridine 5'-diphosphateShow
GO Classification
Component
cell part
membrane part
intrinsic to membrane
integral to membrane
Function
transferase activity, transferring glycosyl groups
UDP-glycosyltransferase activity
acetylglucosaminyltransferase activity
phosphatidylinositol N-acetylglucosaminyltransferase activity
catalytic activity
transferase activity
Process
metabolic process
macromolecule metabolic process
macromolecule modification
protein modification process
protein amino acid lipidation
GPI anchor biosynthetic process
Gene Properties
Chromosome Locationchromosome 16
LocusYPL076W
Gene Sequence>843 bp ATGACAAGATCTCCCTGGAAGCGCCTACTATGGTTGAAACAGGAGTACCCAGATAATTAT ACAGATCCAAGTTTTATTGAGTTGAGAGCAAGACAAAAGGCTGAGAGTAACCAGAAGTCT GATAGAAAATTATCAGAAGCTGCTCGCGCTCAAATTAGGTTGGATTTTATAAGTTTCTAC CAAACCATATTGAACACTTCTTTCATTTACATCACTTTTACATATATTTACTATTATGGC TTCGATCCTATTCCGCCAACTATTTTCCTTTCATTTATAACATTGATTATATCAAGGACG AAAGTCGACCCTCTATTGTCCTCATTCATGGACGTAAAGTCTTCGCTGATTATCACATTT GCAATGTTGACTCTCTCTCCAGTCCTCAAATCTCTTTCTAAAACAACTGCATCTGATTCC ATATGGACATTGTCTTTTTGGCTGACCCTATGGTACATTTTCGTTATTTCGTCAACAAAG TCCAAAGATAAACCCTCTAACCTTTCCACCAATATACTTGTCGCCCTTGTTGCTGTCCTA TCATCGAGGCTTTCGACCACGATCGACGTATTCTGTTTTCTTTTAATTTGTATTCAGTTG AATATCATTCTACCCACTTATTTATCGGTGACGAATAAGGTAGTACCAATAATTTCAAAT ATTATTGTATACTCATTTTTGAATGTTGCTCTAGGTTGGATTTATATGCTGTTGATTTTC TTTGCTTCAGTATTTTATATTACTGTTTTACCTAAGTGGTTCATCTACTGGAAAATCAAT TATCATAAACGGGATAACGATCTACTAAGTACATGGGATGCAAGAACACCAATATTGGAT TAG
Protein Properties
Pfam Domain Function
Protein Residues280
Protein Molecular Weight32576.80078
Protein Theoretical pI9.77
Signalling Regions
  • None
Transmembrane Regions
  • 54-74
  • 76-96
  • 109-129
  • 136-156
  • 190-210
  • 221-241
Protein Sequence>Phosphatidylinositol N-acetylglucosaminyltransferase GPI2 subunit MTRSPWKRLLWLKQEYPDNYTDPSFIELRARQKAESNQKSDRKLSEAARAQIRLDFISFY QTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLIITF AMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSKDKPSNLSTNILVALVAVL SSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVPIISNIIVYSFLNVALGWIYMLLIF FASVFYITVLPKWFIYWKINYHKRDNDLLSTWDARTPILD
References
External Links
ResourceLink
Saccharomyces Genome Database GPI2
Uniprot IDP46961
Uniprot NameGPI2_YEAST
GenBank Gene IDAY692961
Genebank Protein ID51013373
General Reference
  • Leidich, S. D., Kostova, Z., Latek, R. R., Costello, L. C., Drapp, D. A., Gray, W., Fassler, J. S., Orlean, P. (1995). "Temperature-sensitive yeast GPI anchoring mutants gpi2 and gpi3 are defective in the synthesis of N-acetylglucosaminyl phosphatidylinositol. Cloning of the GPI2 gene." J Biol Chem 270:13029-13035.7768896
  • Uemura, H., Jigami, Y. (1995). "A new essential gene GCR4 located in the upstream region of GCR1." Yeast 11:1093-1101.7502585
  • Bussey, H., Storms, R. K., Ahmed, A., Albermann, K., Allen, E., Ansorge, W., Araujo, R., Aparicio, A., Barrell, B., Badcock, K., Benes, V., Botstein, D., Bowman, S., Bruckner, M., Carpenter, J., Cherry, J. M., Chung, E., Churcher, C., Coster, F., Davis, K., Davis, R. W., Dietrich, F. S., Delius, H., DiPaolo, T., Hani, J., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Nature 387:103-105.9169875
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Sobering, A. K., Watanabe, R., Romeo, M. J., Yan, B. C., Specht, C. A., Orlean, P., Riezman, H., Levin, D. E. (2004). "Yeast Ras regulates the complex that catalyzes the first step in GPI-anchor biosynthesis at the ER." Cell 117:637-648.15163411
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258