Probable phospholipid-transporting ATPase NEO1 (P40527)

Identification

Name

Probable phospholipid-transporting ATPase NEO1

Synonyms

Not Available

Gene Name

NEO1

Enzyme Class

3.6.3.1

Biological Properties

General Function

Involved in nucleotide binding

Specific Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids (Potential). Leads to neomycin-resistance when overexpressed. Required for traffic between late Golgi and early endosomes

Cellular Location

Endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein

SMPDB Pathways

Not Available

KEGG Pathways

Not Available

SMPDB Reactions

Not Available

KEGG Reactions

Not Available

Metabolites

YMDB ID	Name	View
YMDB00109	Adenosine triphosphate	Show
YMDB00890	water	Show
YMDB00907	phosphate	Show
YMDB00914	ADP	Show

GO Classification

Component
cell part
membrane
membrane part
intrinsic to membrane
integral to membrane
Function
hydrolase activity
substrate-specific transmembrane transporter activity
ion transmembrane transporter activity
magnesium ion binding
binding
ATPase activity, coupled to transmembrane movement of ions
catalytic activity
nucleoside binding
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism
purine nucleoside binding
hydrolase activity, acting on acid anhydrides
adenyl nucleotide binding
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
adenyl ribonucleotide binding
ATP binding
ion binding
cation binding
substrate-specific transporter activity
metal ion binding
lipid transporter activity
transporter activity
phospholipid transporter activity
transmembrane transporter activity
phospholipid-translocating ATPase activity
Process
lipid transport
nucleoside phosphate metabolic process
transport
phospholipid transport
nucleotide metabolic process
purine nucleotide metabolic process
purine nucleotide biosynthetic process
metabolic process
purine nucleoside triphosphate biosynthetic process
purine ribonucleoside triphosphate biosynthetic process
ATP biosynthetic process
nitrogen compound metabolic process
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleobase, nucleoside and nucleotide metabolic process
establishment of localization

Gene Properties

Chromosome Location

chromosome 9

Locus

YIL048W

Gene Sequence

>3456 bp ATGCCTAACCCTCCTTCATTTAAGTCACATAAGCAGAATTTATTCAACTCCAACAATAAT CAACACGCAAACAGTGTAGATTCTTTTGACTTACATCTCGACGATTCATTTGACGCTGCT TTAGACAGTTTACAAATTAATAATAACCCAGAACCACTTAGTAAGCATAACACTGTTGGA GATAGGGAAAGTTTTGAAATGAGAACAGTAGATGATTTAGACAATTTTTCTAACCATTCT TCGGATTCGCATCGTAAGTCTTCAAACACAGATACCCATCCTTTGATGTATGATAATCGC CTCTCGCAGGATGATAATTTCAAATTTACTAATATTGCTTCGTCACCACCGTCGTCTTCA AACAATATTTTTTCAAAAGCCCTCTCATATTTAAAAGTATCTAACACAAAAAACTGGTCC AAGTTTGGTTCACCCATAGAACTCAGTGATCAACACATCGAAAGAGAAATACATCCAGAC ACGACTCCTGTGTACGATAGGAATAGATACGTTTCGAATGAACTATCTAATGCCAAATAT AATGCCGTTACTTTTGTGCCAACCCTATTATATGAACAGTTCAAATTCTTTTACAATTTA TACTTTTTAGTAGTTGCATTATCGCAGGCCGTTCCTGCACTAAGAATAGGGTATTTATCA TCGTATATTGTACCTTTAGCATTTGTGCTGACAGTAACCATGGCAAAGGAAGCTATAGAT GATATTCAGAGAAGGAGACGTGATAGAGAGTCAAACAATGAACTGTATCATGTAATAACG AGAAATCGTTCCATACCAAGCAAAGACTTAAAAGTTGGAGATCTCATAAAAGTCCATAAA GGTGATCGTATTCCCGCTGATTTGGTTCTTTTACAATCAAGTGAACCATCAGGCGAGTCA TTTATCAAAACTGACCAACTAGATGGGGAGACTGACTGGAAACTACGTGTTGCCTGTCCC TTGACCCAAAATCTATCTGAAAATGACTTAATTAACAGAATTAGTATAACAGCCTCTGCT CCTGAGAAATCTATTCATAAATTTTTAGGTAAAGTTACCTATAAGGATTCCACTTCAAAC CCTTTGTCTGTAGATAATACTCTATGGGCGAATACTGTTCTAGCTTCCAGTGGATTTTGT ATTGCTTGTGTCGTCTACACTGGCAGAGATACTAGGCAAGCTATGAATACAACTACAGCT AAGGTTAAAACTGGCTTATTAGAGCTAGAAATTAACAGTATTTCCAAGATTCTTTGTGCA TGTGTTTTTGCCCTGTCAATTCTTTTAGTTGCATTTGCCGGTTTCCACAATGACGACTGG TACATTGACATTTTGAGGTATCTGATATTGTTTTCTACCATCATCCCTGTCTCTTTAAGA GTTAATTTAGATTTAGCCAAAAGTGTTTATGCACATCAGATCGAACACGATAAAACCATT CCTGAAACAATTGTTAGAACGAGTACAATTCCAGAGGATTTAGGCCGTATTGAATACCTT CTAAGCGACAAAACAGGGACGCTTACTCAGAATGATATGCAATTGAAGAAAATACACTTG GGTACTGTATCATACACGTCGGAAACATTAGACATTGTATCCGACTATGTACAATCTCTG GTGAGCTCTAAAAATGACTCTTTGAACAATTCTAAAGTTGCCTTATCAACAACAAGAAAG GATATGTCTTTTCGTGTACGTGATATGATTTTAACTCTAGCAATTTGCCATAACGTTACT CCCACTTTTGAGGATGACGAACTTACATATCAAGCGGCATCACCTGATGAAATTGCTATT GTTAAATTTACCGAGTCGGTTGGCTTATCTCTTTTCAAAAGAGATCGCCATTCAATTTCT TTATTACACGAACATTCTGGTAAGACTTTAAATTACGAAATTTTACAAGTATTCCCATTT AATTCTGATTCTAAGCGTATGGGTATCATTGTTCGTGATGAGCAATTGGATGAATATTGG TTTATGCAAAAAGGGGCCGATACTGTCATGTCAAAAATTGTGGAAAGCAATGATTGGCTA GAGGAAGAAACAGGTAATATGGCTCGTGAAGGTCTACGTACTTTGGTTATCGGTCGTAAA AAATTAAACAAGAAAATTTACGAGCAATTTCAAAAAGAATACAACGATGCCTCTTTGTCA ATGCTAAATCGTGATCAACAGATGAGTCAAGTTATCACCAAGTATTTAGAGCATGATCTT GAATTATTGGGTCTAACTGGTGTAGAAGATAAGTTACAGAAGGATGTGAAGTCCTCTATC GAGTTATTAAGAAACGCAGGTATCAAGATTTGGATGCTGACAGGTGATAAAGTTGAAACT GCTCGTTGTGTTTCCATAAGTGCAAAATTAATATCTCGTGGCCAATATGTTCATACGATA ACAAAAGTCACAAGACCGGAAGGTGCATTCAATCAGCTGGAGTATCTAAAAATCAATAGA AATGCTTGTCTTTTAATAGATGGTGAATCCCTGGGAATGTTTTTGAAACATTACGAACAG GAATTTTTTGATGTCGTGGTTCATTTACCCACTGTTATTGCTTGCCGTTGTACACCTCAA CAGAAGGCAGACGTTGCTTTGGTAATTCGTAAAATGACGGGCAAAAGGGTATGCTGTATT GGTGATGGAGGTAACGATGTTAGTATGATTCAATGTGCAGATGTTGGCGTAGGGATTGTG GGTAAAGAAGGTAAACAAGCTTCTTTAGCTGCTGATTTTTCTATAACGCAATTTTGTCAC CTCACCGAATTACTATTATGGCATGGTAGGAACTCTTACAAAAGATCTGCTAAATTGGCT CAATTTGTCATGCATAGAGGTTTAATCATTGCAATTTGCCAAGCGGTATATTCCATCTGT TCGTTGTTCGAACCTATTGCTCTTTATCAAGGTTGGTTAATGGTCGGTTATGCTACCTGC TATACAATGGCTCCAGTCTTTTCATTAACTCTTGATCACGATATCGAAGAGTCATTGACT AAAATATACCCAGAATTGTACAAAGAATTGACTGAAGGTAAGAGCTTATCATATAAGACG TTCTTTGTTTGGGTTCTATTATCGCTATTCCAAGGTTCTGTAATTCAATTATTTTCTCAG GCCTTTACGAGTTTATTAGATACTGATTTCACAAGGATGGTTGCTATCAGCTTCACTGCT TTGGTTGTAAATGAATTAATAATGGTTGCCTTAGAAATCTACACATGGAATAAAACAATG CTCGTCACTGAAATTGCTACTTTACTATTTTACATTGTATCGGTTCCATTTCTTGGCGAT TATTTTGATTTAGGTTATATGACTACCGTAAATTACTATGCTGGACTTTTAGTTATATTA CTCATCTCGATCTTTCCTGTCTGGACGGCAAAGGCAATTTATAGAAGGCTACATCCTCCA AGTTATGCAAAAGTGCAAGAGTTTGCCACTCCATAA

Protein Properties

Pfam Domain Function

E1-E2_ATPase (PF00122 )
Hydrolase (PF00702 )

Protein Residues

1151

Protein Molecular Weight

130217.0

Protein Theoretical pI

6.43

Signalling Regions

None

Transmembrane Regions

185-205
210-230
368-388
417-437
439-459
948-968
971-991
1021-1041
1053-1073
1079-1099
1110-1130

Protein Sequence

>Probable phospholipid-transporting ATPase NEO1 MPNPPSFKSHKQNLFNSNNNQHANSVDSFDLHLDDSFDAALDSLQINNNPEPLSKHNTVG DRESFEMRTVDDLDNFSNHSSDSHRKSSNTDTHPLMYDNRLSQDDNFKFTNIASSPPSSS NNIFSKALSYLKVSNTKNWSKFGSPIELSDQHIEREIHPDTTPVYDRNRYVSNELSNAKY NAVTFVPTLLYEQFKFFYNLYFLVVALSQAVPALRIGYLSSYIVPLAFVLTVTMAKEAID DIQRRRRDRESNNELYHVITRNRSIPSKDLKVGDLIKVHKGDRIPADLVLLQSSEPSGES FIKTDQLDGETDWKLRVACPLTQNLSENDLINRISITASAPEKSIHKFLGKVTYKDSTSN PLSVDNTLWANTVLASSGFCIACVVYTGRDTRQAMNTTTAKVKTGLLELEINSISKILCA CVFALSILLVAFAGFHNDDWYIDILRYLILFSTIIPVSLRVNLDLAKSVYAHQIEHDKTI PETIVRTSTIPEDLGRIEYLLSDKTGTLTQNDMQLKKIHLGTVSYTSETLDIVSDYVQSL VSSKNDSLNNSKVALSTTRKDMSFRVRDMILTLAICHNVTPTFEDDELTYQAASPDEIAI VKFTESVGLSLFKRDRHSISLLHEHSGKTLNYEILQVFPFNSDSKRMGIIVRDEQLDEYW FMQKGADTVMSKIVESNDWLEEETGNMAREGLRTLVIGRKKLNKKIYEQFQKEYNDASLS MLNRDQQMSQVITKYLEHDLELLGLTGVEDKLQKDVKSSIELLRNAGIKIWMLTGDKVET ARCVSISAKLISRGQYVHTITKVTRPEGAFNQLEYLKINRNACLLIDGESLGMFLKHYEQ EFFDVVVHLPTVIACRCTPQQKADVALVIRKMTGKRVCCIGDGGNDVSMIQCADVGVGIV GKEGKQASLAADFSITQFCHLTELLLWHGRNSYKRSAKLAQFVMHRGLIIAICQAVYSIC SLFEPIALYQGWLMVGYATCYTMAPVFSLTLDHDIEESLTKIYPELYKELTEGKSLSYKT FFVWVLLSLFQGSVIQLFSQAFTSLLDTDFTRMVAISFTALVVNELIMVALEIYTWNKTM LVTEIATLLFYIVSVPFLGDYFDLGYMTTVNYYAGLLVILLISIFPVWTAKAIYRRLHPP SYAKVQEFATP

References

External Links

Resource	Link
Saccharomyces Genome Database	NEO1
Uniprot ID	P40527
Uniprot Name	ATC7_YEAST
GenBank Gene ID	Z38060
Genebank Protein ID	557820

General Reference

Churcher, C., Bowman, S., Badcock, K., Bankier, A., Brown, D., Chillingworth, T., Connor, R., Devlin, K., Gentles, S., Hamlin, N., Harris, D., Horsnell, T., Hunt, S., Jagels, K., Jones, M., Lye, G., Moule, S., Odell, C., Pearson, D., Rajandream, M., Rice, P., Rowley, N., Skelton, J., Smith, V., Barrell, B., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Nature 387:84-87.9169870
Wicky, S., Schwarz, H., Singer-Kruger, B. (2004). "Molecular interactions of yeast Neo1p, an essential member of the Drs2 family of aminophospholipid translocases, and its role in membrane trafficking within the endomembrane system." Mol Cell Biol 24:7402-7418.15314152
Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956