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Identification
NameFlavohemoprotein
Synonyms
  • Flavohemoglobin
  • Hemoglobin-like protein
  • Nitric oxide dioxygenase
  • NO oxygenase
  • NOD
Gene NameYHB1
Enzyme Class
Biological Properties
General FunctionInvolved in oxidoreductase activity
Specific FunctionIn the presence of oxygen and NADH, it has NADH oxidase activity, which leads to the generation of superoxide and H(2)O(2). Under anaerobic conditions, it also exhibits nitric oxide reductase and FAD reductase activities. However, all these reactions are much lower than NOD activity
Cellular LocationCytoplasm
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00981NitrateShow
YMDB00982noShow
GO Classification
Component
Not Available
Function
heme binding
oxygen binding
catalytic activity
binding
ion binding
cation binding
metal ion binding
transition metal ion binding
iron ion binding
oxidoreductase activity
Process
transport
gas transport
oxygen transport
metabolic process
oxidation reduction
establishment of localization
Gene Properties
Chromosome Locationchromosome 7
LocusYGR234W
Gene Sequence>1200 bp ATGCTAGCCGAAAAAACCCGTTCCATAATCAAAGCAACCGTTCCTGTTTTGGAACAGCAG GGCACTGTCATCACGCGTACATTCTACAAAAATATGCTTACTGAACACACCGAATTACTT AATATTTTTAACAGAACTAATCAAAAGGTTGGCGCACAACCAAATGCCTTGGCCACTACT GTTTTGGCAGCGGCTAAGAACATTGACGATTTGTCTGTGCTTATGGACCATGTCAAGCAA ATTGGCCACAAGCATCGTGCCTTGCAAATTAAACCTGAGCATTATCCAATCGTTGGTGAG TACTTGTTAAAAGCAATCAAGGAAGTTTTAGGCGATGCTGCGACTCCAGAGATAATTAAT GCATGGGGCGAGGCTTACCAAGCAATTGCTGACATCTTCATCACTGTTGAAAAAAAAATG TACGAAGAAGCTTTATGGCCAGGTTGGAAACCATTTGACATTACTGCCAAAGAGTACGTT GCTTCAGATATTGTTGAATTTACTGTTAAGCCAAAGTTCGGTTCTGGTATTGAATTGGAA AGCTTGCCAATCACTCCAGGTCAGTATATTACGGTAAACACTCACCCAATTAGACAGGAA AACCAATACGACGCTTTAAGACACTACTCTCTTTGTTCTGCTTCAACTAAAAATGGTTTA CGATTTGCAGTAAAGATGGAAGCTGCCAGAGAAAATTTTCCTGCAGGATTAGTCTCTGAA TATTTACACAAGGACGCTAAAGTTGGTGATGAAATTAAGCTTAGTGCTCCGGCTGGTGAT TTCGCAATAAATAAAGAGTTGATACACCAAAACGAAGTTCCATTGGTGTTACTATCGTCT GGTGTTGGTGTTACACCACTTTTAGCTATGTTAGAAGAACAAGTCAAATGCAACCCAAAT AGGCCAATTTACTGGATTCAATCTTCCTATGATGAAAAGACTCAAGCATTCAAAAAACAT GTAGATGAACTATTAGCTGAATGTGCGAATGTCGACAAAATAATTGTTCATACTGATACT GAACCACTGATTAATGCTGCATTCTTGAAGGAAAAGTCACCTGCGCATGCCGATGTCTAC ACTTGCGGCTCTTTAGCTTTTATGCAAGCTATGATTGGTCATCTAAAAGAACTAGAGCAT CGTGATGATATGATTCACTATGAACCATTTGGTCCAAAGATGTCAACCGTGCAAGTTTAG
Protein Properties
Pfam Domain Function
Protein Residues399
Protein Molecular Weight44646.0
Protein Theoretical pI6.24
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Flavohemoprotein MLAEKTRSIIKATVPVLEQQGTVITRTFYKNMLTEHTELLNIFNRTNQKVGAQPNALATT VLAAAKNIDDLSVLMDHVKQIGHKHRALQIKPEHYPIVGEYLLKAIKEVLGDAATPEIIN AWGEAYQAIADIFITVEKKMYEEALWPGWKPFDITAKEYVASDIVEFTVKPKFGSGIELE SLPITPGQYITVNTHPIRQENQYDALRHYSLCSASTKNGLRFAVKMEAARENFPAGLVSE YLHKDAKVGDEIKLSAPAGDFAINKELIHQNEVPLVLLSSGVGVTPLLAMLEEQVKCNPN RPIYWIQSSYDEKTQAFKKHVDELLAECANVDKIIVHTDTEPLINAAFLKEKSPAHADVY TCGSLAFMQAMIGHLKELEHRDDMIHYEPFGPKMSTVQV
References
External Links
ResourceLink
Saccharomyces Genome Database YHB1
Uniprot IDP39676
Uniprot NameFHP_YEAST
GenBank Gene IDAY692964
Genebank Protein ID51013379
General Reference
  • Zhu, H., Riggs, A. F. (1992). "Yeast flavohemoglobin is an ancient protein related to globins and a reductase family." Proc Natl Acad Sci U S A 89:5015-5019.1594608
  • van der Aart, Q. J., Kleine, K., Steensma, H. Y. (1996). "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII." Yeast 12:385-390.8701610
  • Tettelin, H., Agostoni Carbone, M. L., Albermann, K., Albers, M., Arroyo, J., Backes, U., Barreiros, T., Bertani, I., Bjourson, A. J., Bruckner, M., Bruschi, C. V., Carignani, G., Castagnoli, L., Cerdan, E., Clemente, M. L., Coblenz, A., Coglievina, M., Coissac, E., Defoor, E., Del Bino, S., Delius, H., Delneri, D., de Wergifosse, P., Dujon, B., Kleine, K., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Nature 387:81-84.9169869
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Crawford, M. J., Sherman, D. R., Goldberg, D. E. (1995). "Regulation of Saccharomyces cerevisiae flavohemoglobin gene expression." J Biol Chem 270:6991-6996.7896850
  • Zhao, X. J., Raitt, D., V Burke, P., Clewell, A. S., Kwast, K. E., Poyton, R. O. (1996). "Function and expression of flavohemoglobin in Saccharomyces cerevisiae. Evidence for a role in the oxidative stress response." J Biol Chem 271:25131-25138.8810268
  • Buisson, N., Labbe-Bois, R. (1998). "Flavohemoglobin expression and function in Saccharomyces cerevisiae. No relationship with respiration and complex response to oxidative stress." J Biol Chem 273:9527-9533.9545281
  • Gardner, P. R., Gardner, A. M., Martin, L. A., Dou, Y., Li, T., Olson, J. S., Zhu, H., Riggs, A. F. (2000). "Nitric-oxide dioxygenase activity and function of flavohemoglobins. sensitivity to nitric oxide and carbon monoxide inhibition." J Biol Chem 275:31581-31587.10922365
  • Liu, L., Zeng, M., Hausladen, A., Heitman, J., Stamler, J. S. (2000). "Protection from nitrosative stress by yeast flavohemoglobin." Proc Natl Acad Sci U S A 97:4672-4676.10758168
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956