Probable phospholipid-transporting ATPase DRS2 (P39524)

Identification

Name

Probable phospholipid-transporting ATPase DRS2

Synonyms

Not Available

Gene Name

DRS2

Enzyme Class

3.6.3.1

Biological Properties

General Function

Involved in nucleotide binding

Specific Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids (Potential). Seems to be involved in ribosome assembly

Cellular Location

Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein

SMPDB Pathways

Not Available

KEGG Pathways

Not Available

SMPDB Reactions

Not Available

KEGG Reactions

Not Available

Metabolites

YMDB ID	Name	View
YMDB00109	Adenosine triphosphate	Show
YMDB00890	water	Show
YMDB00907	phosphate	Show
YMDB00914	ADP	Show

GO Classification

Component
cell part
membrane
membrane part
intrinsic to membrane
integral to membrane
Function
ion transmembrane transporter activity
binding
magnesium ion binding
nucleoside binding
ATPase activity, coupled to transmembrane movement of ions
catalytic activity
purine nucleoside binding
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism
adenyl nucleotide binding
hydrolase activity, acting on acid anhydrides
adenyl ribonucleotide binding
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
ATP binding
ion binding
cation binding
metal ion binding
substrate-specific transporter activity
transporter activity
lipid transporter activity
transmembrane transporter activity
phospholipid transporter activity
phospholipid-translocating ATPase activity
hydrolase activity
substrate-specific transmembrane transporter activity
Process
purine nucleotide biosynthetic process
metabolic process
purine nucleoside triphosphate biosynthetic process
purine ribonucleoside triphosphate biosynthetic process
ATP biosynthetic process
nitrogen compound metabolic process
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleobase, nucleoside and nucleotide metabolic process
establishment of localization
nucleoside phosphate metabolic process
transport
lipid transport
nucleotide metabolic process
phospholipid transport
purine nucleotide metabolic process

Gene Properties

Chromosome Location

chromosome 1

Locus

YAL026C

Gene Sequence

>4068 bp ATGAATGACGACAGAGAAACCCCCCCAAAGAGGAAACCTGGGGAGGACGACACGCTTTTC GATATTGACTTCTTAGACGACACGACTTCTCATTCTGGATCCCGTTCAAAGGTCACCAAC TCACATGCGAACGGATATTATATACCACCAAGTCATGTACTTCCCGAAGAGACTATCGAC TTAGACGCTGATGATGATAACATTGAAAATGATGTCCATGAAAATCTATTTATGAGCAAT AACCATGACGACCAAACGTCTTGGAATGCCAATAGATTCGACTCAGATGCATATCAACCA CAATCCCTACGAGCAGTCAAGCCTCCCGGTTTATTTGCTAGGTTTGGTAATGGTCTCAAA AATGCCTTTACGTTCAAACGGAAGAAAGGGCCCGAAAGTTTCGAAATGAATCATTACAAT GCGGTGACTAATAACGAATTAGACGATAATTATCTAGACTCGAGGAATAAGTTCAACATT AAAATTCTTTTCAACCGGTACATTTTAAGAAAGAACGTTGGCGACGCAGAGGGCAATGGT GAACCAAGAGTCATCCACATCAACGATTCTCTCGCTAATTCCTCCTTCGGTTATAGTGAC AACCATATATCTACCACCAAGTATAATTTTGCGACCTTTCTGCCTAAATTTTTGTTCCAA GAATTTTCCAAATACGCTAATCTGTTTTTTTTATGTACATCTGCCATTCAACAGGTGCCT CACGTCTCGCCAACTAATAGATACACCACAATTGGTACTTTATTAGTGGTTTTGATTGTT TCTGCCATGAAGGAATGTATCGAAGATATCAAGAGAGCTAATTCTGATAAAGAATTAAAT AATTCGACAGCAGAAATATTTTCAGAAGCACATGATGACTTTGTTGAGAAACGATGGATT GATATTCGTGTAGGTGACATAATTAGAGTAAAATCAGAGGAACCTATTCCTGCTGATACC ATAATTTTGTCATCTTCGGAACCGGAGGGTCTTTGCTACATTGAAACTGCCAACTTGGAT GGTGAAACAAATTTGAAAATCAAACAGTCGAGAGTAGAAACTGCCAAATTTATAGACGTT AAAACTTTGAAGAACATGAACGGAAAAGTTGTTTCCGAACAGCCGAACTCTAGCTTGTAT ACTTATGAAGGTACAATGACTTTAAATGATCGTCAAATACCGTTATCTCCTGACCAAATG ATTTTAAGAGGTGCAACTTTAAGAAATACTGCATGGATATTTGGTTTAGTTATCTTCACT GGTCATGAAACTAAGTTATTGCGTAATGCTACTGCAACCCCAATTAAAAGAACCGCGGTT GAGAAAATTATCAACAGACAGATTATTCGATTGTTCACAGTTTTAATCGTGCTAATTTTA ATTTCTTCAATTGGTAATGTTATTATGTCTACTGCAGATGCCAAACATTTATCGTACCTT TACCTGGAGGGTACCAACAAGGCTGGCTTATTCTTCAAAGACTTTTTAACATTTTGGATT CTATTTTCGAATCTAGTTCCTATTTCTCTATTTGTCACCGTTGAATTAATCAAATATTAT CAGGCTTTTATGATAGGTTCAGATCTAGATTTGTACTACGAAAAAACTGATACCCCAACT GTGGTTCGTACATCCTCTTTGGTTGAAGAACTTGGTCAAATAGAATATATATTCAGTGAC AAGACAGGAACTTTAACAAGAAATATTATGGAATTTAAATCCTGCTCTATTGCAGGCCAT TGCTATATTGATAAAATACCTGAAGATAAAACGGCCACTGTTGAAGATGGGATTGAAGTT GGTTATAGAAAATTTGACGATTTGAAGAAAAAGTTAAATGATCCCTCTGATGAGGATTCA CCTATTATCAATGACTTCTTAACATTACTGGCTACCTGTCATACTGTCATTCCAGAATTT CAAAGCGATGGATCTATTAAATATCAAGCAGCCTCTCCAGATGAAGGTGCCCTCGTTCAA GGTGGTGCAGATTTAGGGTATAAGTTTATCATCCGTAAAGGAAACTCTGTAACTGTTTTA TTGGAGGAAACTGGCGAGGAAAAAGAGTATCAACTACTTAACATTTGTGAATTTAATTCC ACCAGGAAGAGAATGAGCGCTATATTTAGATTTCCGGATGGTTCGATAAAGTTATTCTGT AAAGGTGCTGATACTGTCATTCTGGAAAGATTGGATGATGAAGCTAATCAATATGTAGAA GCTACAATGAGACATTTAGAAGATTATGCATCTGAGGGTTTGCGGACGTTGTGTTTGGCG ATGAGAGATATCTCTGAAGGAGAATATGAAGAATGGAATAGCATTTATAATGAGGCTGCC ACAACATTAGATAACAGAGCCGAGAAGCTAGACGAAGCCGCAAATCTAATCGAGAAAAAT CTAATATTAATTGGTGCAACTGCTATTGAAGATAAGTTACAGGACGGTGTTCCAGAAACT ATCCACACATTACAAGAAGCGGGTATTAAAATTTGGGTTTTAACTGGTGACAGACAGGAA ACTGCTATTAACATTGGTATGAGTTGCCGTTTATTGAGTGAAGACATGAATTTGTTGATC ATCAACGAGGAAACCAGAGATGATACTGAGAGAAATTTGTTAGAGAAGATTAACGCTCTA AACGAGCATCAATTGTCAACACATGATATGAAATCCTTAGCGCTCGTCATTGATGGGAAG TCATTAGGCTTTGCCTTGGAACCAGAATTAGAAGATTATTTGCTAACTGTGGCGAAGCTT TGTAAAGCGGTTATATGTTGCCGTGTATCTCCACTACAGAAAGCCTTGGTTGTTAAAATG GTAAAAAGAAAGTCGTCTTCACTACTGCTAGCCATTGCGAGTGGTGCCAACGATGTTAGT ATGATACAGGCAGCTCATGTTGGTGTCGGTATTAGTGGTATGGAAGGTATGCAAGCGGCT CGTTCAGCTGATATAGCTCTTGGCCAATTTAAATTTTTAAAAAAACTATTACTTGTCCAT GGCTCCTGGTCTTATCAAAGAATTTCTGTCGCAATTTTGTACTCTTTTTACAAGAATACA GCATTATACATGACGCAGTTTTGGTACGTTTTTGCCAATGCCTTTTCAGGTCAATCCATT ATGGAATCATGGACAATGAGTTTCTACAACTTATTCTTCACTGTTTGGCCCCCTTTTGTC ATTGGTGTATTTGATCAATTTGTTAGTAGTAGATTACTTGAGCGGTACCCACAATTGTAC AAATTAGGACAGAAGGGTCAATTTTTCTCTGTTTACATCTTCTGGGGATGGATTATTAAT GGCTTTTTCCATTCTGCAATAGTATTTATTGGCACCATATTGATTTACAGATATGGGTTT GCCTTAAATATGCACGGCGAACTAGCTGATCATTGGTCTTGGGGTGTAACTGTCTACACT ACAAGTGTCATTATTGTTTTGGGAAAGGCTGCTTTGGTAACTAATCAATGGACGAAATTC ACCTTAATTGCCATTCCTGGTTCCCTCTTATTTTGGTTAATATTCTTCCCAATTTATGCT TCTATATTTCCTCATGCTAACATCTCAAGAGAGTATTATGGTGTGGTTAAACACACGTAT GGATCCGGTGTATTTTGGTTAACTTTGATCGTTTTACCAATTTTTGCACTGGTAAGAGAT TTTCTATGGAAGTACTATAAAAGAATGTATGAACCAGAAACGTATCATGTTATTCAAGAA ATGCAGAAATACAATATCAGCGACTCTAGGCCGCACGTTCAGCAATTTCAAAATGCCATC AGGAAGGTGAGGCAAGTGCAAAGAATGAAAAAACAAAGAGGATTTGCCTTTTCACAGGCT GAAGAGGGTGGGCAAGAAAAAATTGTCAGAATGTATGATACTACTCAAAAGAGAGGTAAG TACGGTGAATTACAAGATGCTTCGGCGAACCCCTTTAATGACAATAATGGACTGGGAAGT AACGACTTTGAAAGTGCAGAACCGTTCATTGAAAATCCATTTGCTGATGGTAATCAAAAT TCAAATAGATTCAGTTCTTCGAGAGATGATATTTCATTTGATATATGA

Protein Properties

Pfam Domain Function

E1-E2_ATPase (PF00122 )
Hydrolase (PF00702 )

Protein Residues

1355

Protein Molecular Weight

153843.0

Protein Theoretical pI

5.25

Signalling Regions

None

Transmembrane Regions

222-242
247-267
450-470
491-511
1013-1033
1044-1064
1095-1115
1132-1152
1162-1182
1203-1223

Protein Sequence

>Probable phospholipid-transporting ATPase DRS2 MNDDRETPPKRKPGEDDTLFDIDFLDDTTSHSGSRSKVTNSHANGYYIPPSHVLPEETID LDADDDNIENDVHENLFMSNNHDDQTSWNANRFDSDAYQPQSLRAVKPPGLFARFGNGLK NAFTFKRKKGPESFEMNHYNAVTNNELDDNYLDSRNKFNIKILFNRYILRKNVGDAEGNG EPRVIHINDSLANSSFGYSDNHISTTKYNFATFLPKFLFQEFSKYANLFFLCTSAIQQVP HVSPTNRYTTIGTLLVVLIVSAMKECIEDIKRANSDKELNNSTAEIFSEAHDDFVEKRWI DIRVGDIIRVKSEEPIPADTIILSSSEPEGLCYIETANLDGETNLKIKQSRVETAKFIDV KTLKNMNGKVVSEQPNSSLYTYEGTMTLNDRQIPLSPDQMILRGATLRNTAWIFGLVIFT GHETKLLRNATATPIKRTAVEKIINRQIIRLFTVLIVLILISSIGNVIMSTADAKHLSYL YLEGTNKAGLFFKDFLTFWILFSNLVPISLFVTVELIKYYQAFMIGSDLDLYYEKTDTPT VVRTSSLVEELGQIEYIFSDKTGTLTRNIMEFKSCSIAGHCYIDKIPEDKTATVEDGIEV GYRKFDDLKKKLNDPSDEDSPIINDFLTLLATCHTVIPEFQSDGSIKYQAASPDEGALVQ GGADLGYKFIIRKGNSVTVLLEETGEEKEYQLLNICEFNSTRKRMSAIFRFPDGSIKLFC KGADTVILERLDDEANQYVEATMRHLEDYASEGLRTLCLAMRDISEGEYEEWNSIYNEAA TTLDNRAEKLDEAANLIEKNLILIGATAIEDKLQDGVPETIHTLQEAGIKIWVLTGDRQE TAINIGMSCRLLSEDMNLLIINEETRDDTERNLLEKINALNEHQLSTHDMKSLALVIDGK SLGFALEPELEDYLLTVAKLCKAVICCRVSPLQKALVVKMVKRKSSSLLLAIASGANDVS MIQAAHVGVGISGMEGMQAARSADIALGQFKFLKKLLLVHGSWSYQRISVAILYSFYKNT ALYMTQFWYVFANAFSGQSIMESWTMSFYNLFFTVWPPFVIGVFDQFVSSRLLERYPQLY KLGQKGQFFSVYIFWGWIINGFFHSAIVFIGTILIYRYGFALNMHGELADHWSWGVTVYT TSVIIVLGKAALVTNQWTKFTLIAIPGSLLFWLIFFPIYASIFPHANISREYYGVVKHTY GSGVFWLTLIVLPIFALVRDFLWKYYKRMYEPETYHVIQEMQKYNISDSRPHVQQFQNAI RKVRQVQRMKKQRGFAFSQAEEGGQEKIVRMYDTTQKRGKYGELQDASANPFNDNNGLGS NDFESAEPFIENPFADGNQNSNRFSSSRDDISFDI

References

External Links

Resource	Link
Saccharomyces Genome Database	DRS2
Uniprot ID	P39524
Uniprot Name	ATC3_YEAST
GenBank Gene ID	L01795
Genebank Protein ID	171114

General Reference

Ripmaster, T. L., Vaughn, G. P., Woolford, J. L. Jr (1993). "DRS1 to DRS7, novel genes required for ribosome assembly and function in Saccharomyces cerevisiae." Mol Cell Biol 13:7901-7912.8247005
Bussey, H., Kaback, D. B., Zhong, W., Vo, D. T., Clark, M. W., Fortin, N., Hall, J., Ouellette, B. F., Keng, T., Barton, A. B., et, a. l. .. (1995). "The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Proc Natl Acad Sci U S A 92:3809-3813.7731988
Gall, W. E., Geething, N. C., Hua, Z., Ingram, M. F., Liu, K., Chen, S. I., Graham, T. R. (2002). "Drs2p-dependent formation of exocytic clathrin-coated vesicles in vivo." Curr Biol 12:1623-1627.12372257
Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
Saito, K., Fujimura-Kamada, K., Furuta, N., Kato, U., Umeda, M., Tanaka, K. (2004). "Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae." Mol Biol Cell 15:3418-3432.15090616
Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956