Palmitoyltransferase AKR1 (P39010)

Identification
NamePalmitoyltransferase AKR1
Synonyms
  • Ankyrin repeat-containing protein AKR1
Gene NameAKR1
Enzyme ClassNot Available
Biological Properties
General FunctionInvolved in identical protein binding
Specific FunctionPalmitoyltransferase specific for casein kinase 1. Palmitoylates isoforms YCK1 and YCK2 at both C-terminal cysteine residues, which is required for their proper plasma membrane localization. Required for constitutive endocytosis of a-factor receptor STE3 and both constitutive and pheromone-induced endocytosis of alpha-factor receptor STE2
Cellular LocationEarly endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG Reactions
Palmityl-CoA + protein ↔ conjugated protein + Coenzyme A
Metabolites
YMDB IDNameView
GO Classification
Function
cation binding
metal ion binding
transition metal ion binding
zinc ion binding
binding
ion binding
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>2295 bp ATGGTGAACGAATTAGAGAATGTGCCACGGGCGTCGACCTTGACGAATGAAGAGCAAACG GTTGATCCTAGCAATAACGACTCACAGGAAGATATTAGTCTGGGTGATTCTAACGAAATT ACTTCGTTGGCATCATTAAAGGCTATCCGATCAGGAAATGAAGAGGAAAGCGGGAACGAG CAGGTGAATCATAATGATGAGGCCGAAGAAGATCCTCTGTTAACTCGCTACCATACCGCT TGCCAAAGAGGTGATCTAGCGACTGTCAAAGAAATGATACATGGAAAGTTGCTCGAAGTT AATAACGACGGTGACTCTACGGAACATATCACTGGTTTGCATTGGGCAAGTATTAATAAC AGACTTTCTGTAGTGGATTTTCTAGTTTCACAAGGCGCTGATGTCAATGCAAGGGCTGGT GCTTTGCATGCCACACCATTACATTGGGCTGCAAGGTATGGGTACGTGTACATTGTCGAT TTTTTGCTTAAGCATGGAGCTGATCCCACCATGACCGATGACCAAGGTTTCAATCTACTA CACTTATCTGTGAACAGCTCAAATATAATGCTAGTTCTATACGTTTTATTCAACGTTGTC AGCAAAGGTCTTTTAGATATAGATTGCCGAGACCCAAAGGGCAGAACTTCACTACTATGG GCTGCTTACCAGGGAGATTCCCTAACTGTAGCAGAATTGTTGAAATTTGGTGCCAGCATA AAGATCGCAGATACAGAAGGATTCACCCCACTGCACTGGGGCACTGTAAAAGGGCAACCA CATGTACTAAAATACTTGATTCAAGATGGTGCAGATTTTTTCCAAAAAACGGATACCGGA AAAGATTGCTTTGCTATTGCTCAAGAAATGAACACTGTGTACTCACTTAGAGAGGCTCTA ACACATTCTGGATTCGACTACCATGGTTATCCCATTAAAAAATGGTTCAAAAAAAGTCAA CATGCTAAGCTCGTTACATTCATAACGCCATTTCTATTTCTAGGAATCGCATTTGCTCTT TTCTCTCATATTAACCCTTTGTTTGTAATCATTGTCCTTTTTTTATTGGCCATTGCCACT AACAAAGGCTTGAACAAATTCGTTCTGCCGTCATATGGACGAATGGGTGTCCACAACGTT ACCTTGTTAAGGTCACCTTTGTTATCCGGTGTTTTTTTTGGCACTTTACTTTGGGTGACT ATCGTTTGGTTTTTCAAAGTTATGCCTCGAACATTTTCTGATGAACAATATACTAACATC CTAATGCTGGTAATACTTGTTTCTGTATTTTACCTTTTTGGGCAGCTTGTTATTATGGAC CCAGGATGTTTGCCAGAAGAAACTGATCATGAGAACGTGCGACAAACAATCTCGAACTTG CTCGAAATCGGTAAATTTGATACGAAGAACTTTTGTATCGAAACTTGGATTAGAAAACCT CTAAGAAGCAAATTTTCGCCCTTAAACAATGCTGTGGTTGCAAGATTTGATCATTACTGC CCTTGGATCTTTAATGATGTCGGACTCAAAAATCACAAAGCATTTATATTTTTTATTACA TTAATGGAAAGTGGTATTTTCACATTTCTTGCACTTTGCCTGGAATATTTTGATGAACTT GAAGATGCACACGAAGATACATCCCAGAAAAATGGCAAATGTTTCATCCTAGGCGCTTCA GATCTCTGTTCGGGATTAATATATGATAGATTTGTCTTTCTGATATTATTATGGGCTTTA CTCCAATCTATTTGGGTTGCAAGTTTGATCTTCGTCCAAGCCTTTCAAATATGTAAAGGA ATGACCAATACAGAATTCAATGTTTTGATGAAAGAGAGTAAGTCCATTGGCCCCGATGGG TTATCTTTCAATGAAAACTTCAATACTACTCCAGAAGGATTTGCGCCGTCGATTGATCCG GGTGAGGAGAGTAATGATACTGTACTAGCACCAGTCCCAGGCTCAACGATAAGGAAACCA CGCACTTGTTTTGGCGTTTGTTACGCGGTGACTGGTATGGATCAGTGGTTAGCGGTTATA AAAGAAACCATTGGCATCAAGGATAGCACCGGACATAATGTGTATTCGATTACATCAAGA ATACCAACAAATTACGGTTGGAAAAGAAATGTTAAAGACTTTTGGCTTACAAGCGATATA AATGCGCCTTTGTGGAGGCGAATTTTGTATCCTCCTTCGGGCTCTAAAGCTTTGCTCAAC GGGATAGAAGTTGACTATTTTAAGCTTTACAAATTGCCAAATAAGGATGTTGAACAAGGA AATGATATGGTATGA
Protein Properties
Pfam Domain Function
Protein Residues764
Protein Molecular Weight85839.5
Protein Theoretical pI5.48
Signalling Regions
  • None
Transmembrane Regions
  • 322-341
  • 347-364
  • 385-405
  • 419-439
  • 514-534
  • 571-591
Protein Sequence>Palmitoyltransferase AKR1 MVNELENVPRASTLTNEEQTVDPSNNDSQEDISLGDSNEITSLASLKAIRSGNEEESGNE QVNHNDEAEEDPLLTRYHTACQRGDLATVKEMIHGKLLEVNNDGDSTEHITGLHWASINN RLSVVDFLVSQGADVNARAGALHATPLHWAARYGYVYIVDFLLKHGADPTMTDDQGFNLL HLSVNSSNIMLVLYVLFNVVSKGLLDIDCRDPKGRTSLLWAAYQGDSLTVAELLKFGASI KIADTEGFTPLHWGTVKGQPHVLKYLIQDGADFFQKTDTGKDCFAIAQEMNTVYSLREAL THSGFDYHGYPIKKWFKKSQHAKLVTFITPFLFLGIAFALFSHINPLFVIIVLFLLAIAT NKGLNKFVLPSYGRMGVHNVTLLRSPLLSGVFFGTLLWVTIVWFFKVMPRTFSDEQYTNI LMLVILVSVFYLFGQLVIMDPGCLPEETDHENVRQTISNLLEIGKFDTKNFCIETWIRKP LRSKFSPLNNAVVARFDHYCPWIFNDVGLKNHKAFIFFITLMESGIFTFLALCLEYFDEL EDAHEDTSQKNGKCFILGASDLCSGLIYDRFVFLILLWALLQSIWVASLIFVQAFQICKG MTNTEFNVLMKESKSIGPDGLSFNENFNTTPEGFAPSIDPGEESNDTVLAPVPGSTIRKP RTCFGVCYAVTGMDQWLAVIKETIGIKDSTGHNVYSITSRIPTNYGWKRNVKDFWLTSDI NAPLWRRILYPPSGSKALLNGIEVDYFKLYKLPNKDVEQGNDMV
References
External Links
ResourceLink
Saccharomyces Genome Database AKR1
Uniprot IDP39010
Uniprot NameAKR1_YEAST
GenBank Gene IDL31407
Genebank Protein ID466522
General Reference
  • Kao, L. R., Peterson, J., Ji, R., Bender, L., Bender, A. (1996). "Interactions between the ankyrin repeat-containing protein Akr1p and the pheromone response pathway in Saccharomyces cerevisiae." Mol Cell Biol 16:168-178.8524293
  • Jacq, C., Alt-Morbe, J., Andre, B., Arnold, W., Bahr, A., Ballesta, J. P., Bargues, M., Baron, L., Becker, A., Biteau, N., Blocker, H., Blugeon, C., Boskovic, J., Brandt, P., Bruckner, M., Buitrago, M. J., Coster, F., Delaveau, T., del Rey, F., Dujon, B., Eide, L. G., Garcia-Cantalejo, J. M., Goffeau, A., Gomez-Peris, A., Zaccaria, P., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature 387:75-78.9169867
  • Givan, S. A., Sprague, G. F. Jr (1997). "The ankyrin repeat-containing protein Akr1p is required for the endocytosis of yeast pheromone receptors." Mol Biol Cell 8:1317-1327.9243510
  • Feng, Y., Davis, N. G. (2000). "Akr1p and the type I casein kinases act prior to the ubiquitination step of yeast endocytosis: Akr1p is required for kinase localization to the plasma membrane." Mol Cell Biol 20:5350-5359.10866691
  • Roth, A. F., Feng, Y., Chen, L., Davis, N. G. (2002). "The yeast DHHC cysteine-rich domain protein Akr1p is a palmitoyl transferase." J Cell Biol 159:23-28.12370247
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Babu, P., Deschenes, R. J., Robinson, L. C. (2004). "Akr1p-dependent palmitoylation of Yck2p yeast casein kinase 1 is necessary and sufficient for plasma membrane targeting." J Biol Chem 279:27138-27147.15105419
  • Politis, E. G., Roth, A. F., Davis, N. G. (2005). "Transmembrane topology of the protein palmitoyl transferase Akr1." J Biol Chem 280:10156-10163.15632165
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956