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Identification
NameProbable phosphatidylinositol-4-phosphate 5-kinase MSS4
Synonyms
  • 1-phosphatidylinositol-4-phosphate kinase
  • Diphosphoinositide kinase
  • PIP5K
Gene NameMSS4
Enzyme Class
Biological Properties
General FunctionInvolved in phosphatidylinositol phosphate kinase activity
Specific FunctionCatalyzes the phosphorylation of phosphatidylinositol-4- phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol-4,5-biphosphate. Acts downstream of STT4, but in a pathway that does not involve PKC1. May be involved in the organization of the actin cytoskeleton
Cellular LocationNot Available
SMPDB Pathways
Inositol phosphate metabolismPW002495 ThumbThumb?image type=greyscaleThumb?image type=simple
Stress-activated signalling pathways: cell wall stress test 1PW007861 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Inositol phosphate metabolismec00562 Map00562
SMPDB Reactions
PIP(18:0/20:4(5Z,8Z,11Z,14Z)) + phosphatePIP2(18:0/20:4(5Z,8Z,11Z,14Z))
KEGG Reactions
Adenosine triphosphate + 1-phosphatidyl-1D-myo-inositol 4-phosphate + hydronADP + 1D-myo-inositol 4,5-bisphosphate
Adenosine triphosphate + 1-phosphatidyl-1D-myo-inositol 4-phosphate → ADP + 1D-myo-inositol 4,5-bisphosphate
Metabolites
YMDB IDNameView
YMDB00109Adenosine triphosphateShow
YMDB00862hydronShow
YMDB00907phosphateShow
YMDB00914ADPShow
YMDB16197PIP(18:0/20:4(5Z,8Z,11Z,14Z))Show
YMDB16198PIP2(18:0/20:4(5Z,8Z,11Z,14Z))Show
GO Classification
Component
Not Available
Function
lipid kinase activity
phosphatidylinositol phosphate kinase activity
catalytic activity
transferase activity
transferase activity, transferring phosphorus-containing groups
kinase activity
Process
organophosphate metabolic process
phospholipid metabolic process
glycerophospholipid metabolic process
phosphoinositide metabolic process
phosphatidylinositol metabolic process
metabolic process
Gene Properties
Chromosome Locationchromosome 4
LocusYDR208W
Gene Sequence>2340 bp ATGTCAGTCTTGCGATCACAACCTCCTTCAGTTGTACCGCTACATCTGACAACATCCACC AGTCGCAAAACAGAACAGGAACCATCGCTATTGCACTCTGCAATAATTGAGCGACATCAA GACCGTTCGGTGCCGAATTCGAATTCGAATCCGGATTCGAACCATCGAATAAAAAAGGAT CGCAATAATCACACTAGCTACCATTCTTCATCGAATTCGGAGTCAAACATGGAAAGTCCT CGCTTGTCAGATGGTGAGTCTTCCACTCCGACCTCTATTGAAGAGTTAAACCCAACAATA AATAATTCGAGGCTGGTGAAGAGAAACTACTCAATATCAATTGATCCTTTGCATGACAAC AGCAATAATAATACGGATGATGATCATCCAAACACCATAACTTCTCCCCGACCAAATAGC ACTAGTAACAAGGAAATGCAGAAATATAGTTTTCCCGAAGGTAAGGAGTCGAAGAAAATA ACAACACCCTCGTTAAACTCGAATAATTGTTTGGATTTGGACAATTCCAGTCTTGTTCAC ACAGATTCGTATATACAAGACTTAAATGACGATCATATTTTACTAAACAAGCGCGTTTCG AGGCGATCTTCCAGAATATCGGCAGTAACAGCAACTTCCACGACAATTAAGCAAAGAAGA AATACTCAGGATTCAAACCTGCCCAATATTCCCTTTCACGCTTCCAAGCATTCTCAAATT CTACCTATGGATGATTCAGATGTAATAAAATTGGCCAATGGAGATACCAGCATGAAACCA AATTCTGCTACTAAAATTAGTCACTCAATGACTTCTTTGCCTCTTCACCCACTTCCACAA CCTTCTCAAAAGTCAAAGCAATATCATATGATATCCAAATCAACCACTTCTTTGCCGCCA GAGAATGACCACTACTATCAACACAGTCGTGGCACCAACCATAATCACGCTGCTAATGCT GCTGCTGTTAATAATAATACTACTACTACTACCGCTGCTACGGGTCTGAAAAGATCAGAG TCTGCAACGGCAGAAATTAAGAAGATGAGACAATCTTTGCTGCATAAAAGAGAAATGAAA AGGAAAAGAAAAACATTTTTGGTGGATGACGATCGTGTTTTGATCGGTAATAAAGTCAGT GAAGGCCATGTCAACTTTATTATAGCTTATAACATGCTAACTGGTATTCGTGTCGCAGTG TCACGTTGTTCTGGCATAATGAAACCTTTAACTCCGGCAGACTTTAGATTTACGAAGAAG CTTGCCTTTGATTATCACGGAAATGAATTGACCCCTTCTTCTCAGTATGCATTTAAGTTT AAGGACTACTGTCCCGAAGTCTTTAGGGAACTTCGTGCATTATTCGGCTTAGACCCTGCT GATTATTTGGTTTCGTTAACTTCCAAGTACATTTTGAGTGAGTTGAACTCGCCAGGTAAA AGTGGTTCATTTTTTTATTATTCGAGAGATTACAAATATATTATCAAGACCATACATCAT TCTGAGCATATTCATCTGAGAAAGCACATACAAGAATACTATAACCATGTAAGAGACAAT CCAAACACTTTGATTTGTCAATTTTATGGTTTGCATAGAGTGAAAATGCCTATATCGTTT CAAAATAAAATTAAGCATCGGAAAATTTACTTCCTAGTCATGAATAACTTATTTCCACCA CACTTAGACATTCACATTACTTATGATTTAAAAGGTTCCACATGGGGCCGTTTTACCAAT TTGGATAAAGAAAGGTTGGCGAAAGATAGATCATATAGGCCTGTGATGAAAGATTTAAAT TGGCTTGAAGAAGGTCAGAAAATTAAATTGGGTCCATTGAAGAAGAAGACTTTTTTGACA CAGCTGAAAAAAGATGTGGAATTGCTTGCTAAATTGAATACAATGGACTATTCCTTGTTA ATTGGCATTCATGACATCAATAAAGCTAAAGAAGACGACTTACAATTGGCGGATACGGCA TCTATTGAGGAGCAACCACAGACTCAAGGACCCATAAGAACCGGTACCGGAACGGTAGTA CGACATTTTTTTAGAGAGTTTGAAGGTGGAATTCGAGCATCTGATCAATTCAACAATGAT GTGGATTTGATTTATTACGTGGGGATAATCGATTTCTTGACTAATTACTCAGTTATGAAG AAATTAGAAACGTTTTGGAGAAGTCTACGCCATGATACCAAGTTGGTGAGTGCCATACCT CCAAGAGACTACGCCAATAGGTTTTACGAATTCATAGAAGATTCTGTAGATCCTCTGCCC CAGAAAAAAACTCAATCGTCGTATAGAGACGACCCTAACCAGAAAAATTATAAAGACTGA
Protein Properties
Pfam Domain Function
Protein Residues779
Protein Molecular Weight89319.60156
Protein Theoretical pI9.77
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Probable phosphatidylinositol-4-phosphate 5-kinase MSS4 MSVLRSQPPSVVPLHLTTSTSRKTEQEPSLLHSAIIERHQDRSVPNSNSNPDSNHRIKKD RNNHTSYHSSSNSESNMESPRLSDGESSTPTSIEELNPTINNSRLVKRNYSISIDPLHDN SNNNTDDDHPNTITSPRPNSTSNKEMQKYSFPEGKESKKITTPSLNSNNCLDLDNSSLVH TDSYIQDLNDDHILLNKRVSRRSSRISAVTATSTTIKQRRNTQDSNLPNIPFHASKHSQI LPMDDSDVIKLANGDTSMKPNSATKISHSMTSLPLHPLPQPSQKSKQYHMISKSTTSLPP ENDHYYQHSRGTNHNHAANAAAVNNNTTTTTAATGLKRSESATAEIKKMRQSLLHKREMK RKRKTFLVDDDRVLIGNKVSEGHVNFIIAYNMLTGIRVAVSRCSGIMKPLTPADFRFTKK LAFDYHGNELTPSSQYAFKFKDYCPEVFRELRALFGLDPADYLVSLTSKYILSELNSPGK SGSFFYYSRDYKYIIKTIHHSEHIHLRKHIQEYYNHVRDNPNTLICQFYGLHRVKMPISF QNKIKHRKIYFLVMNNLFPPHLDIHITYDLKGSTWGRFTNLDKERLAKDRSYRPVMKDLN WLEEGQKIKFGPLKKKTFLTQLKKDVELLAKLNTMDYSLLIGIHDINKAKEDDLQLADTA SIEEQPQTQGPIRTGTGTVVRHFFREFEGGIRASDQFNNDVDLIYYVGIIDFLTNYSVMK KLETFWRSLRHDTKLVSAIPPRDYANRFYEFIEDSVDPLPQKKTQSSYRDDPNQKNYKD
References
External Links
ResourceLink
Saccharomyces Genome Database MSS4
Uniprot IDP38994
Uniprot NameMSS4_YEAST
GenBank Gene IDD13716
Genebank Protein ID493719
General Reference
  • Yoshida, S., Ohya, Y., Nakano, A., Anraku, Y. (1994). "Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae." Mol Gen Genet 242:631-640.8152413
  • Jacq, C., Alt-Morbe, J., Andre, B., Arnold, W., Bahr, A., Ballesta, J. P., Bargues, M., Baron, L., Becker, A., Biteau, N., Blocker, H., Blugeon, C., Boskovic, J., Brandt, P., Bruckner, M., Buitrago, M. J., Coster, F., Delaveau, T., del Rey, F., Dujon, B., Eide, L. G., Garcia-Cantalejo, J. M., Goffeau, A., Gomez-Peris, A., Zaccaria, P., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature 387:75-78.9169867
  • Desrivieres, S., Cooke, F. T., Parker, P. J., Hall, M. N. (1998). "MSS4, a phosphatidylinositol-4-phosphate 5-kinase required for organization of the actin cytoskeleton in Saccharomyces cerevisiae." J Biol Chem 273:15787-15793.9624178
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956