| Identification |
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| Name | Asparaginyl-tRNA synthetase, cytoplasmic |
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| Synonyms | - Asparagine--tRNA ligase
- AsnRS
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| Gene Name | DED81 |
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| Enzyme Class | |
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| Biological Properties |
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| General Function | Involved in nucleotide binding |
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| Specific Function | ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn) |
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| Cellular Location | Cytoplasm |
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| SMPDB Pathways | Not Available |
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| KEGG Pathways | Not Available |
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| SMPDB Reactions | Not Available |
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| KEGG Reactions | |
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| Metabolites | | YMDB ID | Name | View |
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| YMDB00097 | Adenosine monophosphate | Show | | YMDB00109 | Adenosine triphosphate | Show | | YMDB00219 | Pyrophosphate | Show | | YMDB00226 | L-Asparagine | Show |
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| GO Classification | | Component |
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| intracellular part | | cytoplasm | | cell part | | Function |
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| ligase activity | | binding | | nucleoside binding | | purine nucleoside binding | | nucleic acid binding | | adenyl nucleotide binding | | aspartate-tRNA ligase activity | | adenyl ribonucleotide binding | | asparagine-tRNA ligase activity | | ATP binding | | ligase activity, forming carbon-oxygen bonds | | ligase activity, forming aminoacyl-tRNA and related compounds | | catalytic activity | | aminoacyl-tRNA ligase activity | | nucleotide binding | | Process |
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| ncRNA metabolic process | | biosynthetic process | | tRNA metabolic process | | tRNA aminoacylation | | tRNA aminoacylation for protein translation | | macromolecule biosynthetic process | | cellular macromolecule biosynthetic process | | translation | | aspartyl-tRNA aminoacylation | | asparaginyl-tRNA aminoacylation | | metabolic process | | macromolecule metabolic process | | cellular macromolecule metabolic process | | RNA metabolic process |
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| Gene Properties |
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| Chromosome Location | chromosome 8 |
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| Locus | YHR019C |
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| Gene Sequence | >1665 bp
ATGTCATCTTTGTACATTAAGGAGGCTACCGGTGTAGACGAATTGACCACAGCCGGTTCA
CAAGACCATCCATTCAAAACCCCAGCATATGCTTTGTTCGCATCTCAACAGAAGAGCGAT
GCCACGGAACCTAAGCTTTTCGTGTTTAAGACAGAAGACAACGAGTACCAGGAAATCAGT
GCTTCTGCTTTGAAGAAGGCTCGTAAGGGCTGTGATGGTTTGAAGAAAAAGGCAGTCAAG
CAAAAGGAACAGGAGTTGAAGAAACAACAAAAAGAGGCAGAAAATGCTGCCAAGCAATTG
TCTGCTTTGAATATCACCATTAAGGAGGACGAATCGCTACCAGCTGCCATTAAGACTAGA
ATTTATGACTCTTATTCCAAGGTCGGACAAAGAGTTAAGGTTTCCGGTTGGATCCATAGA
TTACGTTCTAACAAGAAGGTTATTTTCGTCGTCCTCAGAGACGGATCTGGTTTCATTCAA
TGTGTCTTGTCCGGTGATTTGGCATTGGCTCAACAAACTTTGGACCTGACTTTGGAATCC
ACCGTTACTCTGTACGGTACCATAGTCAAATTGCCTGAGGGTAAAACCGCTCCAGGTGGT
GTTGAATTGAATGTCGACTATTACGAAGTTGTAGGTTTGGCCCCCGGTGGTGAAGACTCC
TTTACAAACAAAATCGCAGAGGGCTCAGACCCTTCTTTACTGTTGGACCAACGTCATTTG
GCCTTGAGAGGAGATGCCTTGTCTGCAGTCATGAAAGTCCGTGCTGCTCTACTGAAAAGC
GTTAGACGTGTTTATGATGAAGAACATTTGACAGAAGTTACCCCACCATGTATGGTGCAA
ACTCAAGTCGAAGGTGGTTCCACTTTGTTCAAGATGAACTATTACGGCGAGGAAGCTTAC
TTGACCCAAAGTTCCCAATTATATTTAGAAACCTGTTTGGCCTCCCTAGGTGATGTTTAT
ACCATCCAAGAATCTTTCAGAGCTGAAAAGTCCCACACAAGAAGACATTTGTCCGAATAT
ACCCATATCGAAGCTGAATTGGCCTTCTTGACTTTCGACGATCTATTACAACATATTGAA
ACTTTGATCGTCAAATCCGTGCAATACGTTTTGGAAGACCCAATTGCTGGCCCACTCGTA
AAACAATTGAATCCAAACTTTAAGGCTCCAAAGGCTCCATTCATGAGATTACAGTACAAG
GATGCCATTACCTGGTTGAACGAACACGACATCAAGAACGAAGAGGGCGAAGACTTTAAA
TTTGGTGACGATATTGCAGAAGCTGCTGAAAGAAAGATGACCGATACCATCGGCGTCCCA
ATCTTTTTGACGAGATTCCCAGTAGAAATCAAGTCTTTCTACATGAAGCGTTGTTCTGAC
GACCCCCGCGTCACTGAATCCGTCGACGTTTTGATGCCAAACGTTGGTGAAATCACTGGT
GGTTCTATGAGAATCGACGACATGGACGAACTAATGGCAGGGTTTAAGCGTGAGGGTATT
GATACCGACGCCTACTACTGGTTCATTGACCAAAGAAAATACGGTACTTGCCCACATGGT
GGTTACGGTATCGGTACCGAACGTATTTTAGCCTGGTTGTGTGACAGATTCACTGTCAGA
GACTGTTCCTTGTATCCACGTTTCAGCGGTAGATGTAAGCCATGA |
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| Protein Properties |
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| Pfam Domain Function | |
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| Protein Residues | 554 |
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| Protein Molecular Weight | 62206.30078 |
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| Protein Theoretical pI | 5.59 |
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| Signalling Regions | |
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| Transmembrane Regions | |
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| Protein Sequence | >Asparaginyl-tRNA synthetase, cytoplasmic
MSSLYIKEATGVDELTTAGSQDHPFKTPAYALFASQQKSDATEPKLFVFKTEDNEYQEIS
ASALKKARKGCDGLKKKAVKQKEQELKKQQKEAENAAKQLSALNITIKEDESLPAAIKTR
IYDSYSKVGQRVKVSGWIHRLRSNKKVIFVVLRDGSGFIQCVLSGDLALAQQTLDLTLES
TVTLYGTIVKLPEGKTAPGGVELNVDYYEVVGLAPGGEDSFTNKIAEGSDPSLLLDQRHL
ALRGDALSAVMKVRAALLKSVRRVYDEEHLTEVTPPCMVQTQVEGGSTLFKMNYYGEEAY
LTQSSQLYLETCLASLGDVYTIQESFRAEKSHTRRHLSEYTHIEAELAFLTFDDLLQHIE
TLIVKSVQYVLEDPIAGPLVKQLNPNFKAPKAPFMRLQYKDAITWLNEHDIKNEEGEDFK
FGDDIAEAAERKMTDTIGVPIFLTRFPVEIKSFYMKRCSDDPRVTESVDVLMPNVGEITG
GSMRIDDMDELMAGFKREGIDTDAYYWFIDQRKYGTCPHGGYGIGTERILAWLCDRFTVR
DCSLYPRFSGRCKP |
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| References |
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| External Links | |
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| General Reference | - Johnston, M., Andrews, S., Brinkman, R., Cooper, J., Ding, H., Dover, J., Du, Z., Favello, A., Fulton, L., Gattung, S., et, a. l. .. (1994). "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Science 265:2077-2082.8091229
- Landrieu, I., Vandenbol, M., Leberman, R., Portetelle, D., Hartlein, M. (1998). "Identification of YHR019 in Saccharomyces cerevisiae chromosome VIII as the gene for the cytosolic asparaginyl-tRNA synthetase." Yeast 14:527-533.9605503
- Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956
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