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Ribose-phosphate pyrophosphokinase 3 (P38689)

Identification
NameRibose-phosphate pyrophosphokinase 3
Synonyms
  • Phosphoribosyl pyrophosphate synthase 3
Gene NamePRS3
Enzyme Class
Biological Properties
General FunctionInvolved in magnesium ion binding
Specific Function5-phosphoribose 1-diphosphate synthase involved in nucleotide, histidine, and tryptophan biosynthesis. Active in heteromultimeric complexes with other 5-phosphoribose 1- diphosphate synthases (PRS2, PRS3, PRS4 and PRS5)
Cellular LocationCytoplasm
SMPDB Pathways
Histidine BiosynthesisPW002418 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Pentose phosphate pathwayec00030 Map00030
Purine metabolismec00230 Map00230
SMPDB Reactions
D-Ribose 5-phosphate + Adenosine triphosphate → Adenosine monophosphate + hydron + Phosphoribosyl pyrophosphate
KEGG Reactions
Adenosine triphosphate + alpha-D-ribose 5-phosphate ↔ Adenosine monophosphate + hydron + Phosphoribosyl pyrophosphate
Metabolites
YMDB IDNameView
YMDB00097Adenosine monophosphateShow
YMDB00109Adenosine triphosphateShow
YMDB00119D-Ribose 5-phosphateShow
YMDB00402Phosphoribosyl pyrophosphateShow
YMDB00650alpha-D-ribose 5-phosphateShow
YMDB00862hydronShow
GO Classification
Component
Not Available
Function
ribose phosphate diphosphokinase activity
binding
ion binding
cation binding
metal ion binding
catalytic activity
transferase activity
magnesium ion binding
transferase activity, transferring phosphorus-containing groups
diphosphotransferase activity
Process
nucleobase, nucleoside and nucleotide metabolic process
ribonucleoside monophosphate biosynthetic process
nucleoside phosphate metabolic process
nucleotide metabolic process
biosynthetic process
nucleoside metabolic process
cellular biosynthetic process
metabolic process
nucleoside monophosphate metabolic process
nucleoside monophosphate biosynthetic process
nitrogen compound metabolic process
nucleotide biosynthetic process
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
Gene Properties
Chromosome Locationchromosome 8
LocusYHL011C
Gene Sequence>963 bp ATGCCAACAAATTCCATCAAATTATTAGCACCAGATGTCCACCGAGGTTTAGCTGAACTT GTTGCCAAAAGGCTAGGTCTACAGTTAACAAGCAGCAAACTAAAGAGAGATCCAACCGGT GAGGTTTCCTTTTCTATTGGAGAATCTGTTAGGGACCAAGATATCTTTATCATCACGCAA ATTGGCTCTGGTGTCGTGAACGATCGTGTTCTAGAACTACTGATCATGATCAATGCTTCG AAGACTGCGTCTGCAAGAAGAATCACTGCTATTATTCCAAATTTCCCATATGCAAGACAA GATAGAAAGGATAAGTCTCGTGCTCCTATTACCGCTAAATTGATGGCCGATATGTTAACA ACAGCCGGGTGTGATCATGTTATTACTATGGATTTGCATGCTTCCCAAATTCAAGGGTTC TTCGACGTCCCAGTAGATAATTTATATGCAGAACCAAGCGTAGTTAGGTATATCAAGGAA AATGTCAACTATATGGATTCAATAATAATTTCGCCAGATGCTGGTGGGGCAAAACGTGCT GCCACTTTGGCTGATCGTTTGGATCTAAATTTTGCATTGATCCATAAGGAAAGAGCTCGT GCCAATGAGGTTTCTCGTATGGTTCTTGTTGGGGATGTTACTGATAAGATTTGTATCATT GTTGATGATATGGCCGATACTTGTGGTACTTTAGCTAAGGCTGCTGAAATTCTTTTGGAG AATAGAGCTAAATCTGTTATTGCTATTGTCACGCACGGTGTTTTATCAGGCAGAGCTATT GAAAATATTAACAATTCTAAACTGGATAGAGTTGTTTGCACAAATACTGTTCCATTTGAA GAAAAAATCAAGAAGTGCCCAAAACTGGCAGTCATTGACATCAGTAGTGTGCTGGCTGAA AGTATTCGTCGTTTGCATAATGGTGAAAGTATTTCTTATTTATTTAAGAATTATCCCTTA TAA
Protein Properties
Pfam Domain Function
Protein Residues320
Protein Molecular Weight35123.30078
Protein Theoretical pI8.47
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Ribose-phosphate pyrophosphokinase 3 MPTNSIKLLAPDVHRGLAELVAKRLGLQLTSSKLKRDPTGEVSFSIGESVRDQDIFIITQ IGSGVVNDRVLELLIMINASKTASARRITAIIPNFPYARQDRKDKSRAPITAKLMADMLT TAGCDHVITMDLHASQIQGFFDVPVDNLYAEPSVVRYIKENVNYMDSIIISPDAGGAKRA ATLADRLDLNFALIHKERARANEVSRMVLVGDVTDKICIIVDDMADTCGTLAKAAEILLE NRAKSVIAIVTHGVLSGRAIENINNSKLDRVVCTNTVPFEEKIKKCPKLAVIDISSVLAE SIRRLHNGESISYLFKNYPL
References
External Links
ResourceLink
Saccharomyces Genome Database PRS3
Uniprot IDP38689
Uniprot NameKPR3_YEAST
GenBank Gene IDAY693094
Genebank Protein ID51013639
General Reference
  • Carter, A. T., Narbad, A., Pearson, B. M., Beck, K. F., Logghe, M., Contreras, R., Schweizer, M. (1994). "Phosphoribosylpyrophosphate synthetase (PRS): a new gene family in Saccharomyces cerevisiae." Yeast 10:1031-1044.7992503
  • Johnston, M., Andrews, S., Brinkman, R., Cooper, J., Ding, H., Dover, J., Du, Z., Favello, A., Fulton, L., Gattung, S., et, a. l. .. (1994). "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Science 265:2077-2082.8091229
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Hernando, Y., Carter, A. T., Parr, A., Hove-Jensen, B., Schweizer, M. (1999). "Genetic analysis and enzyme activity suggest the existence of more than one minimal functional unit capable of synthesizing phosphoribosyl pyrophosphate in Saccharomyces cerevisiae." J Biol Chem 274:12480-12487.10212224
  • Binley, K. M., Radcliffe, P. A., Trevethick, J., Duffy, K. A., Sudbery, P. E. (1999). "The yeast PRS3 gene is required for cell integrity, cell cycle arrest upon nutrient deprivation, ion homeostasis and the proper organization of the actin cytoskeleton." Yeast 15:1459-1469.10514564
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Hove-Jensen, B. (2004). "Heterooligomeric phosphoribosyl diphosphate synthase of Saccharomyces cerevisiae: combinatorial expression of the five PRS genes in Escherichia coli." J Biol Chem 279:40345-40350.15280369