Trehalose synthase complex regulatory subunit TSL1 (P38427)

Identification
NameTrehalose synthase complex regulatory subunit TSL1
Synonyms
  • Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 123 kDa subunit
Gene NameTSL1
Enzyme ClassNot Available
Biological Properties
General FunctionCarbohydrate transport and metabolism
Specific FunctionRegulatory subunit of the trehalose synthase complex that catalyzes the production of trehalose from glucose-6- phosphate and UDP-glucose in a two step process. May stabilize the trehalose synthase complex, and confer sensitivity to physiological concentrations of phosphate and to fructose 6- phosphate
Cellular LocationCytoplasm
SMPDB Pathways
Starch and sucrose metabolismPW002481 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Starch and sucrose metabolismec00500 Map00500
SMPDB Reactions
Uridine diphosphate glucose + alpha-D-glucose 6 phosphate → Uridine 5'-diphosphate + Trehalose 6-phosphate
Trehalose 6-phosphate + water ↔ Trehalose + phosphate
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00008TrehaloseShow
YMDB00137Trehalose 6-phosphateShow
YMDB00212Uridine diphosphate glucoseShow
YMDB00307Uridine 5'-diphosphateShow
YMDB00890waterShow
YMDB00907phosphateShow
YMDB16263alpha-D-glucose 6 phosphateShow
GO Classification
Function
catalytic activity
Process
metabolic process
primary metabolic process
carbohydrate metabolic process
oligosaccharide metabolic process
disaccharide metabolic process
trehalose metabolic process
trehalose biosynthetic process
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>3297 bp ATGGCTCTCATCGTGGCATCTTTGTTTTTGCCCTACCAACCACAATTCGAGCTTGACACC TCTCTCCCTGAGAACTCGCAGGTGGACTCATCTCTCGTGAACATCCAGGCTATGGCCAAT GACCAACAGCAACAACGTGCGCTTTCTAACAACATCTCACAGGAATCATTGGTCGCGCCA GCACCAGAACAAGGTGTCCCCCCAGCAATCTCAAGGAGTGCCACCAGGTCACCCAGTGCT TTCAACCGCGCCTCGTCTACGACAAATACTGCCACTTTAGATGATCTTGTCTCTTCGGAC ATATTCATGGAAAACTTGACTGCGAATGCAACTACCTCACATACGCCAACAAGCAAGACT ATGCTTAAACCCCGGAAAAATGGTTCCGTGGAACGATTCTTCTCCCCTTCTTCCAATATT CCCACGGATCGCATCGCATCGCCAATCCAGCATGAGCATGACTCCGGTTCGAGAATTGCT TCGCCAATCCAACAGCAACAGCAGGACCCCACGACCAACTTATTAAAGAACGTCAACAAG TCATTGTTAGTGCACTCACTGTTGAACAACACCTCACAAACTAGCCTAGAAGGACCCAAC AACCACATTGTTACCCCGAAATCGAGGGCGGGCAACAGGCCTACTTCGGCGGCTACTTCT TTAGTTAATAGGACCAAACAAGGTTCGGCCTCCTCTGGATCTTCTGGGTCTTCTGCGCCA CCTTCCATTAAAAGGATTACGCCCCACTTGACTGCGTCTGCTGCAAAACAGCGTCCCTTA TTGGCTAAACAGCCTTCTAATCTGAAATATTCGGAGTTAGCAGATATTTCGTCGAGTGAG ACGTCTTCGCAGCATAATGAGTCGGACCCGGATGATCTAACTACTGCCCCTGACGAGGAA TATGTTTCTGATTTGGAAATGGATGACGCGAAGCAGGACTACAAGGTTCCAAAGTTCGGC GGCTATTCCAATAAATCTAAACTTAAGAAATATGCGCTGTTAAGGTCATCTCAGGAGCTG TTTAGCCGTCTTCCATGGTCGATCGTTCCCTCTATCAAAGGTAATGGCGCCATGAAGAAC GCCATAAACACTGCAGTCTTGGAGAATATCATTCCGCACCGTCATGTTAAGTGGGTCGGT ACCGTCGGAATCCCAACGGATGAGATTCCGGAAAATATCCTTGCGAACATCTCTGACTCT TTAAAAGACAAGTACGACTCCTATCCTGTCCTTACGGACGACGACACCTTCAAAGCCGCA TACAAAAACTACTGTAAACAAATCTTGTGGCCTACGCTGCATTACCAGATTCCAGACAAT CCGAACTCGAAGGCTTTTGAAGATCACTCTTGGAAGTTCTATAGAAACTTAAACCAAAGG TTTGCGGACGCGATCGTTAAAATCTATAAGAAAGGTGACACCATCTGGATTCATGATTAC CATTTAATGCTGGTTCCGCAGATGGTGAGAGACGTCTTGCCTTTTGCCAAAATAGGATTT ACCTTACATGTCTCGTTCCCCAGTAGTGAAGTGTTTAGGTGTCTGGCTCAGCGTGAGAAG ATCTTAGAAGGCTTGACCGGTGCAGACTTTGTCGGCTTCCAGACGAGGGAGTATGCAAGA CATTTCTTACAGACGTCTAACCGTCTGCTAATGGCGGACGTGGTACATGATGAAGAGCTA AAGTATAACGGCAGAGTCGTTTCTGTGAGGTTCACCCCAGTTGGTATCGACGCCTTTGAT TTGCAATCGCAATTGAAGGATGGAAGTGTCATGCAATGGCGTCAATTGATTCGTGAAAGA TGGCAAGGGAAAAAACTAATTGTGTGTCGTGATCAATTCGATAGAATTAGAGGTATTCAC AAGAAATTGTTGGCTTATGAAAAATTCTTGGTCGAAAATCCGGAATACGTGGAAAAATCG ACTTTAATTCAAATCTGTATTGGAAGCAGTAAGGATGTAGAACTGGAGCGCCAGATCATG ATTGTCGTGGATAGAATCAACTCGCTATCCACCAATATTAGTATTTCTCAACCTGTGGTG TTTTTGCATCAAGATCTAGATTTTTCTCAGTATTTAGCTTTGAGTTCAGAGGCAGATTTG TTCGTAGTCAGCTCTCTAAGGGAAGGTATGAACTTGACATGTCACGAATTTATCGTTTGT TCTGAGGACAAAAATGCTCCCCTACTGTTGTCAGAATTTACTGGTAGTGCATCTTTATTG AATGATGGCGCTATAATAATTAACCCATGGGATACCAAGAACTTCTCACAAGCCATTCTC AAGGGGTTGGAGATGCCATTCGATAAGAGAAGGCCACAGTGGAAGAAATTGATGAAAGAC ATTATCAACAACGACTCTACAAACTGGATCAAGACTTCTTTACAAGATATTCATATTTCG TGGCAATTCAATCAAGAAGGTTCCAAGATCTTCAAATTGAATACAAAAACACTGATGGAA GATTACCAGTCATCTAAAAAGCGTATGTTTGTTTTCAACATTGCTGAACCACCTTCATCG AGAATGATTTCCATACTGAATGACATGACTTCTAAGGGCAATATCGTTTACATCATGAAC TCATTTCCAAAGCCCATTCTGGAAAATCTTTACAGTCGTGTGCAAAACATTGGGTTGATT GCCGAGAATGGTGCATACGTTAGTCTGAACGGTGTATGGTACAACATTGTTGATCAAGTC GATTGGCGTAACGATGTAGCCAAAATTCTCGAGGACAAAGTGGAGAGATTACCTGGCTCG TACTACAAGATAAATGAGTCCATGATCAAGTTCCACACTGAAAATGCGGAAGATCAAGAT CGTGTAGCTAGTGTTATCGGTGATGCCATCACACATATCAATACTGTTTTTGACCACAGA GGTATTCATGCCTACGTTTACAAAAACGTTGTTTCCGTACAACAAGTGGGACTTTCCTTA TCGGCAGCTCAATTTCTTTTCAGATTCTATAATTCTGCTTCGGATCCACTGGATACGAGT TCCGGCCAAATCACAAATATTCAGACACCATCTCAACAAAATCCTTCAGATCAAGAACAA CAACCTCCAGCCTCTCCCACTGTGTCGATGAACCATATTGATTTCGCATGTGTCTCTGGT TCATCGTCTCCTGTGCTTGAACCATTGTTCAAATTGGTCAATGATGAAGCAAGTGAAGGG CAAGTAAAAGCCGGACACGCCATTGTTTATGGTGATGCTACTTCTACTTATGCCAAAGAA CATGTAAATGGGTTAAACGAACTTTTCACGATCATTTCAAGAATCATTGAAGATTAA
Protein Properties
Pfam Domain Function
Protein Residues1098
Protein Molecular Weight123017.0
Protein Theoretical pI6.61
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Trehalose synthase complex regulatory subunit TSL1 MALIVASLFLPYQPQFELDTSLPENSQVDSSLVNIQAMANDQQQQRALSNNISQESLVAP APEQGVPPAISRSATRSPSAFNRASSTTNTATLDDLVSSDIFMENLTANATTSHTPTSKT MLKPRKNGSVERFFSPSSNIPTDRIASPIQHEHDSGSRIASPIQQQQQDPTTNLLKNVNK SLLVHSLLNNTSQTSLEGPNNHIVTPKSRAGNRPTSAATSLVNRTKQGSASSGSSGSSAP PSIKRITPHLTASAAKQRPLLAKQPSNLKYSELADISSSETSSQHNESDPDDLTTAPDEE YVSDLEMDDAKQDYKVPKFGGYSNKSKLKKYALLRSSQELFSRLPWSIVPSIKGNGAMKN AINTAVLENIIPHRHVKWVGTVGIPTDEIPENILANISDSLKDKYDSYPVLTDDDTFKAA YKNYCKQILWPTLHYQIPDNPNSKAFEDHSWKFYRNLNQRFADAIVKIYKKGDTIWIHDY HLMLVPQMVRDVLPFAKIGFTLHVSFPSSEVFRCLAQREKILEGLTGADFVGFQTREYAR HFLQTSNRLLMADVVHDEELKYNGRVVSVRFTPVGIDAFDLQSQLKDGSVMQWRQLIRER WQGKKLIVCRDQFDRIRGIHKKLLAYEKFLVENPEYVEKSTLIQICIGSSKDVELERQIM IVVDRINSLSTNISISQPVVFLHQDLDFSQYLALSSEADLFVVSSLREGMNLTCHEFIVC SEDKNAPLLLSEFTGSASLLNDGAIIINPWDTKNFSQAILKGLEMPFDKRRPQWKKLMKD IINNDSTNWIKTSLQDIHISWQFNQEGSKIFKLNTKTLMEDYQSSKKRMFVFNIAEPPSS RMISILNDMTSKGNIVYIMNSFPKPILENLYSRVQNIGLIAENGAYVSLNGVWYNIVDQV DWRNDVAKILEDKVERLPGSYYKINESMIKFHTENAEDQDRVASVIGDAITHINTVFDHR GIHAYVYKNVVSVQQVGLSLSAAQFLFRFYNSASDPLDTSSGQITNIQTPSQQNPSDQEQ QPPASPTVSMNHIDFACVSGSSSPVLEPLFKLVNDEASEGQVKAGHAIVYGDATSTYAKE HVNGLNELFTIISRIIED
References
External Links
ResourceLink
Saccharomyces Genome Database TSL1
Uniprot IDP38427
Uniprot NameTSL1_YEAST
GenBank Gene IDX72788
Genebank Protein ID288437
General Reference
  • Vuorio, O. E., Kalkkinen, N., Londesborough, J. (1993). "Cloning of two related genes encoding the 56-kDa and 123-kDa subunits of trehalose synthase from the yeast Saccharomyces cerevisiae." Eur J Biochem 216:849-861.8404905
  • Bowman, S., Churcher, C., Badcock, K., Brown, D., Chillingworth, T., Connor, R., Dedman, K., Devlin, K., Gentles, S., Hamlin, N., Hunt, S., Jagels, K., Lye, G., Moule, S., Odell, C., Pearson, D., Rajandream, M., Rice, P., Skelton, J., Walsh, S., Whitehead, S., Barrell, B. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Nature 387:90-93.9169872
  • Reinders, A., Burckert, N., Hohmann, S., Thevelein, J. M., Boller, T., Wiemken, A., De Virgilio, C. (1997). "Structural analysis of the subunits of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae and their function during heat shock." Mol Microbiol 24:687-695.9194697
  • Bell, W., Sun, W., Hohmann, S., Wera, S., Reinders, A., De Virgilio, C., Wiemken, A., Thevelein, J. M. (1998). "Composition and functional analysis of the Saccharomyces cerevisiae trehalose synthase complex." J Biol Chem 273:33311-33319.9837904
  • Ficarro, S. B., McCleland, M. L., Stukenberg, P. T., Burke, D. J., Ross, M. M., Shabanowitz, J., Hunt, D. F., White, F. M. (2002). "Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Nat Biotechnol 20:301-305.11875433
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Gruhler, A., Olsen, J. V., Mohammed, S., Mortensen, P., Faergeman, N. J., Mann, M., Jensen, O. N. (2005). "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Mol Cell Proteomics 4:310-327.15665377
  • Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956