tRNA (cytosine-5-)-methyltransferase NCL1 (P38205)

Identification
NametRNA (cytosine-5-)-methyltransferase NCL1
Synonyms
  • Multisite-specific tRNA:m5C-methyltransferase
  • tRNA methyltransferase 4
Gene NameNCL1
Enzyme Class
Biological Properties
General FunctionInvolved in RNA binding
Specific FunctionMethylates cytosine to m5C at several positions in different tRNAs and pre-tRNAs containing intron. Able to modify tRNAs at all four positions (34, 40, 48 and 49) at which m5C has been found in tRNAs. May be involved in ribosome biogenesis as its disruption leads to increased sensitivity to the antibiotic paromomycin
Cellular LocationNucleus, nucleolus
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00198S-AdenosylhomocysteineShow
YMDB00297S-AdenosylmethionineShow
GO Classification
Component
Not Available
Function
catalytic activity
transferase activity
transferase activity, transferring one-carbon groups
methyltransferase activity
binding
nucleic acid binding
RNA binding
Process
Not Available
Gene Properties
Chromosome Locationchromosome 2
LocusYBL024W
Gene Sequence>2055 bp ATGGCTAGAAGAAAGAATTTCAAAAAAGGGAACAAGAAGACTTTTGGTGCTCGTGATGAC TCGAGAGCTCAAAAAAACTGGTCTGAACTGGTAAAGGAAAATGAAAAATGGGAAAAATAC TATAAGACTTTAGCTCTTTTCCCAGAAGATCAATGGGAAGAATTTAAAAAGACATGTCAA GCTCCACTTCCTCTAACTTTTAGAATTACAGGTTCTAGAAAGCATGCCGGTGAGGTCCTG AATTTGTTTAAAGAAAGACATCTACCAAACTTGACTAATGTTGAGTTTGAAGGTGAGAAG ATTAAGGCCCCTGTAGAATTACCTTGGTATCCAGACCATCTTGCTTGGCAATTGGACGTT CCTAAGACGGTTATTAGAAAGAATGAACAATTCGCAAAAACTCAGAGATTTTTAGTTGTT GAAAATGCCGTTGGTAATATCTCAAGACAAGAAGCCGTTTCAATGATTCCTCCAATCGTT CTAGAAGTAAAACCTCATCACACTGTTTTAGATATGTGTGCTGCTCCTGGCTCCAAAACT GCTCAATTAATCGAAGCCTTGCACAAGGATACAGATGAACCATCTGGTTTCGTTGTAGCT AATGATGCCGATGCCAGAAGATCTCATATGTTGGTTCACCAATTGAAGAGATTGAACAGT GCCAACTTGATGGTTGTCAACCATGACGCCCAATTCTTCCCACGTATCAGATTACATGGC AACTCAAATAACAAGAATGATGTTTTAAAATTTGACAGAATCCTGTGTGACGTTCCATGT TCTGGTGATGGTACCATGAGGAAAAATGTTAATGTTTGGAAAGACTGGAACACACAAGCA GGTCTTGGTTTGCATGCTGTTCAGCTGAATATATTAAACAGGGGTTTGCATCTTCTAAAG AACAACGGTAGATTGGTTTACTCAACCTGTTCTTTAAATCCTATTGAAAATGAAGCGGTT GTTGCCGAAGCGTTAAGAAAGTGGGGTGACAAGATTAGATTAGTTAACTGTGATGATAAG CTTCCTGGCCTAATAAGATCCAAGGGTGTATCCAAATGGCCTGTCTATGACAGAAATTTG ACTGAGAAAACCAAAGGAGACGAAGGTACACTAGATAGTTTCTTTTCACCATCTGAAGAA GAGGCATCGAAATTCAATTTACAAAATTGTATGAGGGTTTATCCTCACCAACAAAACACA GGCGGATTTTTCATTACTGTTTTCGAAAAAGTCGAAGATAGCACTGAGGCGGCTACAGAG AAACTATCTTCTGAAACCCCAGCTCTAGAGTCTGAAGGACCTCAAACAAAGAAAATAAAG GTAGAAGAAGTCCAAAAGAAAGAAAGACTACCACGTGACGCGAACGAAGAGCCTTTTGTT TTCGTTGATCCACAGCACGAAGCTTTAAAAGTTTGTTGGGATTTCTACGGCATCGATAAT ATTTTCGACAGAAACACTTGTTTAGTGCGTAACGCCACTGGTGAACCAACAAGAGTGGTT TACACTGTGTGTCCAGCATTGAAGGATGTTATTCAAGCGAATGACGATAGGTTGAAGATT ATTTATTCTGGTGTAAAATTGTTTGTCTCTCAAAGAAGTGATATCGAATGTTCATGGAGA ATCCAAAGTGAATCATTGCCAATAATGAAACACCATATGAAATCTAATAGAATTGTTGAA GCTAATTTAGAGATGTTAAAACACTTGTTAATCGAATCTTTCCCTAACTTTGACGACATT CGTTCGAAGAACATCGATAATGATTTTGTTGAAAAGATGACAAAATTAAGCTCTGGTTGC GCCTTTATTGATGTGTCAAGAAATGACCCTGCCAAAGAAAACTTATTCTTGCCTGTGTGG AAAGGCAACAAGTGTATCAATTTGATGGTTTGTAAAGAAGATACTCATGAGCTATTATAT AGGATCTTTGGTATTGATGCGAATGCCAAGGCTACTCCAAGCGCTGAAGAAAAAGAAAAA GAAAAAGAAACGACTGAATCTCCCGCAGAAACTACTACCGGAACCTCTACTGAAGCTCCT AGCGCTGCTAATTGA
Protein Properties
Pfam Domain Function
Protein Residues684
Protein Molecular Weight77878.0
Protein Theoretical pI7.39
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>tRNA (cytosine-5-)-methyltransferase NCL1 MARRKNFKKGNKKTFGARDDSRAQKNWSELVKENEKWEKYYKTLALFPEDQWEEFKKTCQ APLPLTFRITGSRKHAGEVLNLFKERHLPNLTNVEFEGEKIKAPVELPWYPDHLAWQLDV PKTVIRKNEQFAKTQRFLVVENAVGNISRQEAVSMIPPIVLEVKPHHTVLDMCAAPGSKT AQLIEALHKDTDEPSGFVVANDADARRSHMLVHQLKRLNSANLMVVNHDAQFFPRIRLHG NSNNKNDVLKFDRILCDVPCSGDGTMRKNVNVWKDWNTQAGLGLHAVQLNILNRGLHLLK NNGRLVYSTCSLNPIENEAVVAEALRKWGDKIRLVNCDDKLPGLIRSKGVSKWPVYDRNL TEKTKGDEGTLDSFFSPSEEEASKFNLQNCMRVYPHQQNTGGFFITVFEKVEDSTEAATE KLSSETPALESEGPQTKKIKVEEVQKKERLPRDANEEPFVFVDPQHEALKVCWDFYGIDN IFDRNTCLVRNATGEPTRVVYTVCPALKDVIQANDDRLKIIYSGVKLFVSQRSDIECSWR IQSESLPIMKHHMKSNRIVEANLEMLKHLLIESFPNFDDIRSKNIDNDFVEKMTKLSSGC AFIDVSRNDPAKENLFLPVWKGNKCINLMVCKEDTHELLYRIFGIDANAKATPSAEEKEK EKETTESPAETTTGTSTEAPSAAN
References
External Links
ResourceLink
Saccharomyces Genome Database NCL1
Uniprot IDP38205
Uniprot NameNCL1_YEAST
GenBank Gene IDX77291
Genebank Protein ID602895
General Reference
  • Van Dyck, L., Jonniaux, J. L., de Melo Barreiros, T., Kleine, K., Goffeau, A. (1994). "Analysis of a 17.4 kb DNA segment of yeast chromosome II encompassing the ribosomal protein L19 as well as proteins with homologies to components of the hnRNP and snRNP complexes and to the human proliferation-associated p120 antigen." Yeast 10:1663-1673.7725803
  • Feldmann, H., Aigle, M., Aljinovic, G., Andre, B., Baclet, M. C., Barthe, C., Baur, A., Becam, A. M., Biteau, N., Boles, E., Brandt, T., Brendel, M., Bruckner, M., Bussereau, F., Christiansen, C., Contreras, R., Crouzet, M., Cziepluch, C., Demolis, N., Delaveau, T., Doignon, F., Domdey, H., Dusterhus, S., Dubois, E., Dujon, B., El Bakkoury, M., Entian, K. D., Feurmann, M., Fiers, W., Fobo, G. M., Fritz, C., Gassenhuber, H., Glandsdorff, N., Goffeau, A., Grivell, L. A., de Haan, M., Hein, C., Herbert, C. J., Hollenberg, C. P., Holmstrom, K., Jacq, C., Jacquet, M., Jauniaux, J. C., Jonniaux, J. L., Kallesoe, T., Kiesau, P., Kirchrath, L., Kotter, P., Korol, S., Liebl, S., Logghe, M., Lohan, A. J., Louis, E. J., Li, Z. Y., Maat, M. J., Mallet, L., Mannhaupt, G., Messenguy, F., Miosga, T., Molemans, F., Muller, S., Nasr, F., Obermaier, B., Perea, J., Pierard, A., Piravandi, E., Pohl, F. M., Pohl, T. M., Potier, S., Proft, M., Purnelle, B., Ramezani Rad, M., Rieger, M., Rose, M., Schaaff-Gerstenschlager, I., Scherens, B., Schwarzlose, C., Skala, J., Slonimski, P. P., Smits, P. H., Souciet, J. L., Steensma, H. Y., Stucka, R., Urrestarazu, A., van der Aart, Q. J., van Dyck, L., Vassarotti, A., Vetter, I., Vierendeels, F., Vissers, S., Wagner, G., de Wergifosse, P., Wolfe, K. H., Zagulski, M., Zimmermann, F. K., Mewes, H. W., Kleine, K. (1994). "Complete DNA sequence of yeast chromosome II." EMBO J 13:5795-5809.7813418
  • Wu, P., Brockenbrough, J. S., Paddy, M. R., Aris, J. P. (1998). "NCL1, a novel gene for a non-essential nuclear protein in Saccharomyces cerevisiae." Gene 220:109-117.9767141
  • Motorin, Y., Grosjean, H. (1999). "Multisite-specific tRNA:m5C-methyltransferase (Trm4) in yeast Saccharomyces cerevisiae: identification of the gene and substrate specificity of the enzyme." RNA 5:1105-1118.10445884
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956