Dehydrodolichyl diphosphate synthase (P35196)

Identification
NameDehydrodolichyl diphosphate synthase
Synonyms
  • DEDOL-PP synthase
Gene NameRER2
Enzyme Class
Biological Properties
General FunctionInvolved in transferase activity, transferring alkyl or aryl (other than methyl) groups
Specific FunctionCis-prenyl transferase that adds multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP)
Cellular LocationEndoplasmic reticulum membrane; Peripheral membrane protein
SMPDB Pathways
Terpenoid backbone biosynthesisPW002485 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Terpenoid backbone biosynthesisec00900 Map00900
SMPDB Reactions
Farnesyl pyrophosphate + Isopentenyl pyrophosphate → di-trans, poly-cis-polyprenyl diphosphate (C80) + Pyrophosphate
KEGG Reactions
Farnesyl pyrophosphate + Isopentenyl pyrophosphate ↔ Pyrophosphate + geranylgeranyl diphosphate
Isopentenyl pyrophosphate + geranylgeranyl diphosphate ↔ Pyrophosphate + pentaprenyl diphosphate + hydron
pentaprenyl diphosphate + Isopentenyl pyrophosphate ↔ Pyrophosphate + hexaprenyl diphosphate
hexaprenyl diphosphate + Isopentenyl pyrophosphate + hydron ↔ Pyrophosphate + heptaprenyl diphosphate
heptaprenyl diphosphate + Isopentenyl pyrophosphate ↔ Pyrophosphate + octaprenyl diphosphate
Metabolites
YMDB IDNameView
YMDB00200Isopentenyl pyrophosphateShow
YMDB00219PyrophosphateShow
YMDB00229Farnesyl pyrophosphateShow
YMDB00614nonaprenyl diphosphateShow
YMDB00631geranylgeranyl diphosphateShow
YMDB00632pentaprenyl diphosphateShow
YMDB00633hexaprenyl diphosphateShow
YMDB00634heptaprenyl diphosphateShow
YMDB00635octaprenyl diphosphateShow
YMDB00636decaprenyl diphosphateShow
YMDB00637undecaprenyl diphosphateShow
YMDB00638dodecaprenyl diphosphateShow
YMDB00639tridecaprenyl diphosphateShow
YMDB00640tetradecaprenyl diphosphateShow
YMDB00641pentadecaprenyl diphosphateShow
YMDB00642hexadecaprenyl diphosphateShow
YMDB00643heptadecaprenyl diphosphateShow
YMDB00644octadecaprenyl diphosphateShow
YMDB00862hydronShow
YMDB16322di-trans, poly-cis-polyprenyl diphosphate (C80)Show
GO Classification
Component
Not Available
Function
catalytic activity
transferase activity
transferase activity, transferring alkyl or aryl (other than methyl) groups
Process
Not Available
Gene Properties
Chromosome Locationchromosome 2
LocusYBR002C
Gene Sequence>861 bp ATGGAAACGGATAGTGGTATACCTGGTCATTCATTTGTGTTAAAGTGGACAAAAAACATC TTTTCGCGCACATTGCGTGCATCTAACTGTGTACCTAGACATGTTGGGTTCATCATGGAT GGGAACAGGAGATTCGCTAGAAAGAAAGAGATGGACGTAAAGGAGGGCCACGAGGCAGGA TTTGTTAGTATGAGTAGAATCTTAGAACTGTGTTATGAAGCAGGAGTCGATACGGCTACC GTGTTTGCCTTTTCAATTGAAAATTTCAAGAGGAGCTCACGGGAAGTTGAATCACTGATG ACTTTAGCGCGCGAAAGGATACGACAAATCACAGAACGTGGAGAGCTGGCCTGTAAGTAT GGGGTACGCATTAAAATTATCGGCGATCTCTCTTTGTTGGATAAGTCTCTATTAGAAGAT GTTCGGGTTGCTGTGGAAACTACAAAGAACAACAAAAGGGCCACGTTAAATATCTGCTTT CCATATACAGGCAGGGAAGAAATCTTGCATGCCATGAAAGAAACAATTGTTCAACATAAG AAGGGCGCCGCTATAGACGAAAGCACGTTAGAATCGCATCTCTACACGGCGGGGGTACCC CCTTTAGATTTATTGATTAGGACAAGTGGCGTTTCCAGATTAAGTGACTTTTTGATATGG CAGGCATCGAGTAAGGGCGTACGCATCGAATTGCTGGATTGTTTATGGCCAGAGTTTGGA CCTATACGGATGGCATGGATTTTATTAAAATTTTCGTTTCACAAATCCTTTTTAAACAAA GAGTACAGATTAGAGGAAGGTGATTATGACGAGGAAACCAATGGGGACCCCATCGATTTG AAAGAAAAAAAGTTGAATTAA
Protein Properties
Pfam Domain Function
Protein Residues286
Protein Molecular Weight32693.30078
Protein Theoretical pI7.91
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Dehydrodolichyl diphosphate synthase METDSGIPGHSFVLKWTKNIFSRTLRASNCVPRHVGFIMDGNRRFARKKEMDVKEGHEAG FVSMSRILELCYEAGVDTATVFAFSIENFKRSSREVESLMTLARERIRQITERGELACKY GVRIKIIGDLSLLDKSLLEDVRVAVETTKNNKRATLNICFPYTGREEILHAMKETIVQHK KGAAIDESTLESHLYTAGVPPLDLLIRTSGVSRLSDFLIWQASSKGVRIELLDCLWPEFG PIRMAWILLKFSFHKSFLNKEYRLEEGDYDEETNGDPIDLKEKKLN
References
External Links
ResourceLink
Saccharomyces Genome Database RER2
Uniprot IDP35196
Uniprot NameRER2_YEAST
GenBank Gene IDAB013497
Genebank Protein ID4126457
General Reference
  • Sato, M., Sato, K., Nishikawa, S., Hirata, A., Kato, J., Nakano, A. (1999). "The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis." Mol Cell Biol 19:471-483.9858571
  • Wolfe, K. H., Lohan, A. J. (1994). "Sequence around the centromere of Saccharomyces cerevisiae chromosome II: similarity of CEN2 to CEN4." Yeast 10 Suppl A:S41-S46.8091860
  • Feldmann, H., Aigle, M., Aljinovic, G., Andre, B., Baclet, M. C., Barthe, C., Baur, A., Becam, A. M., Biteau, N., Boles, E., Brandt, T., Brendel, M., Bruckner, M., Bussereau, F., Christiansen, C., Contreras, R., Crouzet, M., Cziepluch, C., Demolis, N., Delaveau, T., Doignon, F., Domdey, H., Dusterhus, S., Dubois, E., Dujon, B., El Bakkoury, M., Entian, K. D., Feurmann, M., Fiers, W., Fobo, G. M., Fritz, C., Gassenhuber, H., Glandsdorff, N., Goffeau, A., Grivell, L. A., de Haan, M., Hein, C., Herbert, C. J., Hollenberg, C. P., Holmstrom, K., Jacq, C., Jacquet, M., Jauniaux, J. C., Jonniaux, J. L., Kallesoe, T., Kiesau, P., Kirchrath, L., Kotter, P., Korol, S., Liebl, S., Logghe, M., Lohan, A. J., Louis, E. J., Li, Z. Y., Maat, M. J., Mallet, L., Mannhaupt, G., Messenguy, F., Miosga, T., Molemans, F., Muller, S., Nasr, F., Obermaier, B., Perea, J., Pierard, A., Piravandi, E., Pohl, F. M., Pohl, T. M., Potier, S., Proft, M., Purnelle, B., Ramezani Rad, M., Rieger, M., Rose, M., Schaaff-Gerstenschlager, I., Scherens, B., Schwarzlose, C., Skala, J., Slonimski, P. P., Smits, P. H., Souciet, J. L., Steensma, H. Y., Stucka, R., Urrestarazu, A., van der Aart, Q. J., van Dyck, L., Vassarotti, A., Vetter, I., Vierendeels, F., Vissers, S., Wagner, G., de Wergifosse, P., Wolfe, K. H., Zagulski, M., Zimmermann, F. K., Mewes, H. W., Kleine, K. (1994). "Complete DNA sequence of yeast chromosome II." EMBO J 13:5795-5809.7813418
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106