tRNA (uracil-5-)-methyltransferase (P33753)

Identification
NametRNA (uracil-5-)-methyltransferase
Synonyms
  • Transfer RNA methyltransferase 2
  • tRNA(M-5-U54)-methyltransferase
Gene NameTRM2
Enzyme Class
Biological Properties
General FunctionInvolved in RNA methyltransferase activity
Specific FunctionCatalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNA. May also have a role in tRNA stabilization or maturation
Cellular LocationNot Available
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00198S-AdenosylhomocysteineShow
YMDB00297S-AdenosylmethionineShow
YMDB00438S-Adenosyl-L-methionineShow
GO Classification
Component
Not Available
Function
transferase activity
transferase activity, transferring one-carbon groups
methyltransferase activity
RNA methyltransferase activity
catalytic activity
Process
metabolic process
macromolecule metabolic process
cellular macromolecule metabolic process
RNA metabolic process
RNA processing
Gene Properties
Chromosome Locationchromosome 11
LocusYKR056W
Gene Sequence>YKR056W TRM2 SGDID:S000001764, Chr XI from 549448-551367, Verified ORF, "tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA rep ATGTACGAACAGTTTGAATTTTCTTTTTTTTTCTTCGAAAATTCAGACAATAAGGTTAAA TATAAAGCTCATCTCATCTCATCGATAAAACGCTGGAGTATTATCACATGCATGCGTTGC TTTTGGACCGTACAGAAGTCTATATTTAAAGCTAGGTTTTTCGCTTGCAGAAACTTTGTC AAGAAGCATAATTATAAACTAATCAGCACCATGACTGGAAGTACTGAAATGGTACCACCA ACAATGAAACATACCGTTGACAACAAAAGGCTTTCGTCTCCTTTGACAGATTCTGGTAAC CGCCGGACTAAGAAGCCAAAGTTGAGAAAGTACAAGGCCAAAAAGGTTGAAACAACTTCT CCGATGGGTGTCCTAGAATTTGAAGTGAACGATTTGTTAAAATCTCAAAATTTGTCCAGG GAGCAGGTTCTGAACGATGTTACTTCAATTCTAAATGATAAGTCCTCAACAGATGGACCT ATCGTCTTACAATATCACCGAGAGGTAAAAAATGTCAAGGTCTTAGAAATTACTTCCAAT GGCAACGGGTTGGCTTTGATCGATAATCCTGTTGAAACAGAAAAGAAGCAAGTTGTTATC ATACCGTTTGGCCTGCCCGGTGATGTAGTTAATATCAAAGTCTTTAAGACCCACCCTTAC TATGTCGAGAGTGATTTATTAGACGTAGTGGAAAAATCTCCAATGAGAAGAGATGATTTA ATTAGGGATAAATATTTCGGGAAATCTTCAGGAAGTCAATTAGAGTTCTTAACTTACGAT GACCAACTAGAATTGAAAAGAAAAACAATTATGAATGCCTACAAGTTCTTCGCACCAAGG TTAGTTGCTGAAAAACTTTTACCCCCATTTGACACCACCGTAGCTTCCCCTTTACAATTT GGCTACAGGACCAAAATTACGCCTCATTTCGATATGCCAAAAAGAAAACAAAAGGAACTA TCAGTAAGGCCTCCTTTAGGATTTGGTCAAAAGGGTAGACCTCAATGGAGAAAAGATACT TTGGATATCGGCGGACATGGTTCGATATTAGATATCGATGAATGTGTGCTTGCAACTGAA GTTCTCAACAAAGGATTGACTAATGAAAGAAGAAAGTTTGAGCAAGAGTTTAAAAACTAC AAAAAAGGCGCGACTATTTTACTGAGAGAGAATACCACTATTTTAGACCCTTCCAAACCA ACTTTAGAACAGTTAACCGAAGAAGCCTCTAGGGATGAAAATGGTGATATAAGTTATGTC GAAGTCGAAGACAAAAAGAACAATGTCAGGCTGGCTAAAACTTGCGTTACCAATCCTAGA CAAATTGTCACTGAATATGTTGATGGATATACTTTTAATTTTAGTGCGGGTGAGTTTTTT CAAAATAATAACTCTATCCTGCCAATAGTGACCAAGTATGTCCGTGATAACTTGCAAGCT CCCGCTAAAGGTGATGATAATAAAACAAAGTTTCTAGTAGATGCTTATTGTGGATCAGGT CTTTTCAGTATATGCAGCTCCAAGGGCGTAGATAAAGTGATTGGTGTAGAAATTTCCGCT GACAGTGTCTCTTTTGCAGAAAAAAATGCAAAGGCAAATGGTGTTGAAAACTGTAGATTC ATCGTTGGAAAGGCTGAGAAACTCTTTGAGTCTATTGATACTCCAAGTGAAAACACTTCC GTTATCTTGGATCCACCACGCAAGGGCTGTGACGAATTATTCCTAAAGCAATTAGCCGCA TATAATCCAGCCAAGATTATTTACATATCGTGTAATGTCCATTCCCAGGCACGTGATGTC GAGTACTTCCTCAAAGAAACAGAAAACGGTTCCGCCCACCAGATTGAAAGCATAAGAGGA TTTGATTTCTTTCCACAAACGCACCACGTTGAGAGTGTGTGTATAATGAAGAGAATCTAA
Protein Properties
Pfam Domain Function
Protein Residues639
Protein Molecular Weight72851.79688
Protein Theoretical pI9.14
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>tRNA (uracil-5-)-methyltransferase MYEQFEFSFFFFENSDNKVKYKAHLISSIKRWSIITCMRCFWTVQKSIFKARFFACRNFV KKHNYKLISTMTGSTEMVPPTMKHTVDNKRLSSPLTDSGNRRTKKPKLRKYKAKKVETTS PMGVLEFEVNDLLKSQNLSREQVLNDVTSILNDKSSTDGPIVLQYHREVKNVKVLEITSN GNGLALIDNPVETEKKQVVIIPFGLPGDVVNIKVFKTHPYYVESDLLDVVEKSPMRRDDL IRDKYFGKSSGSQLEFLTYDDQLELKRKTIMNAYKFFAPRLVAEKLLPPFDTTVASPLQF GYRTKITPHFDMPKRKQKELSVRPPLGFGQKGRPQWRKDTLDIGGHGSILDIDECVLATE VLNKGLTNERRKFEQEFKNYKKGATILLRENTTILDPSKPTLEQLTEEASRDENGDISYV EVEDKKNNVRLAKTCVTNPRQIVTEYVDGYTFNFSAGEFFQNNNSILPIVTKYVRDNLQA PAKGDDNKTKFLVDAYCGSGLFSICSSKGVDKVIGVEISADSVSFAEKNAKANGVENCRF IVGKAEKLFESIDTPSENTSVILDPPRKGCDELFLKQLAAYNPAKIIYISCNVHSQARDV EYFLKETENGSAHQIESIRGFDFFPQTHHVESVCIMKRI
References
External Links
ResourceLink
Saccharomyces Genome Database TRM2
Uniprot IDP33753
Uniprot NameTRM2_YEAST
General Reference
  • Van Vliet-Reedijk, J. C., Planta, R. J. (1993). "The RHO4a and NUD1 genes on Saccharomyces cerevisiae chromosome XI." Yeast 9:1139-1147.8256523
  • Nordlund, M. E., Johansson, J. O., von Pawel-Rammingen, U., Bystrom, A. S. (2000). "Identification of the TRM2 gene encoding the tRNA(m5U54)methyltransferase of Saccharomyces cerevisiae." RNA 6:844-860.10864043
  • Dujon, B., Alexandraki, D., Andre, B., Ansorge, W., Baladron, V., Ballesta, J. P., Banrevi, A., Bolle, P. A., Bolotin-Fukuhara, M., Bossier, P., et, a. l. .. (1994). "Complete DNA sequence of yeast chromosome XI." Nature 369:371-378.8196765
  • Chow, T. Y., Perkins, E. L., Resnick, M. A. (1992). "Yeast RNC1 encodes a chimeric protein, RhoNUC, with a human rho motif and deoxyribonuclease activity." Nucleic Acids Res 20:5215-5221.1408836
  • Brachat, S., Dietrich, F. S., Voegeli, S., Zhang, Z., Stuart, L., Lerch, A., Gates, K., Gaffney, T., Philippsen, P. (2003). "Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Genome Biol 4:R45.12844361
  • Johansson, M. J., Bystrom, A. S. (2002). "Dual function of the tRNA(m(5)U54)methyltransferase in tRNA maturation." RNA 8:324-335.12003492
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956