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Identification
NameProbable phospholipid-transporting ATPase DNF1
SynonymsNot Available
Gene NameDNF1
Enzyme Class
Biological Properties
General FunctionInvolved in nucleotide binding
Specific FunctionThis magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids (Potential)
Cellular LocationMembrane; Multi-pass membrane protein
SMPDB Pathways
Lysolipid incorporation into ERPW002532 ThumbThumb?image type=greyscaleThumb?image type=simple
Lysolipid incorporation into ER PC(10:0/10:0)PW002780 ThumbThumb?image type=greyscaleThumb?image type=simple
Lysolipid incorporation into ER PC(14:0/14:0)PW002783 ThumbThumb?image type=greyscaleThumb?image type=simple
Lysolipid incorporation into ER PC(16:0/16:0)PW002784 ThumbThumb?image type=greyscaleThumb?image type=simple
Lysolipid incorporation into ER PC(16:1(11Z)/16:1(11Z))PW002786 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG PathwaysNot Available
SMPDB Reactions
Adenosine triphosphate + water + PC(14:0/0:0)ADP + phosphate + PC(14:0/0:0)
Adenosine triphosphate + water + PE(14:0/0:0)ADP + phosphate + PE(14:0/0:0)
Adenosine triphosphate + water + Lyso-PS(14:0/0:0) → ADP + phosphate + Lyso-PS(14:0/0:0)
Lyso-PI(14:0/0:0) + Adenosine triphosphate + water → Lyso-PI(14:0/0:0) + ADP + phosphate
Lyso-PS(0:0/14:0) + Adenosine triphosphate + waterLyso-PS(0:0/14:0) + ADP + phosphate
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00109Adenosine triphosphateShow
YMDB00890waterShow
YMDB00907phosphateShow
YMDB00914ADPShow
YMDB01175PC(14:0/0:0)Show
YMDB01194PE(14:0/0:0)Show
YMDB13347Lyso-PI(0:0/14:0)Show
YMDB13365Lyso-PS(0:0/14:0)Show
GO Classification
Component
intrinsic to membrane
integral to membrane
cell part
membrane
membrane part
Function
nucleoside binding
purine nucleoside binding
hydrolase activity
substrate-specific transporter activity
adenyl nucleotide binding
substrate-specific transmembrane transporter activity
lipid transporter activity
adenyl ribonucleotide binding
ion transmembrane transporter activity
phospholipid transporter activity
ATP binding
magnesium ion binding
phospholipid-translocating ATPase activity
ion binding
ATPase activity, coupled to transmembrane movement of ions
catalytic activity
cation binding
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism
metal ion binding
hydrolase activity, acting on acid anhydrides
transporter activity
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
transmembrane transporter activity
binding
Process
establishment of localization
purine ribonucleoside triphosphate biosynthetic process
transport
ATP biosynthetic process
nitrogen compound metabolic process
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleobase, nucleoside and nucleotide metabolic process
lipid transport
nucleoside phosphate metabolic process
metabolic process
phospholipid transport
nucleotide metabolic process
purine nucleotide metabolic process
purine nucleotide biosynthetic process
purine nucleoside triphosphate biosynthetic process
Gene Properties
Chromosome Locationchromosome 5
LocusYER166W
Gene Sequence>4716 bp ATGTCTGGAACTTTTCATGGCGATGGGCATGCTCCCATGTCGCCCTTTGAAGATACATTT CAATTTGAAGATAATAGCAGTAATGAAGATACACATATTGCACCTACCCATTTTGATGAT GGTGCAACAAGCAACAAATACAGCCGGCCACAGGTCAGCTTCAATGATGAAACACCCAAA AATAAACGTGAAGATGCAGAAGAATTCACATTTAACGATGACACAGAATATGACAATCAT TCCTTTCAGCCAACACCGAAACTTAATAATGGATCTGGGACGTTTGATGATGTGGAATTA GACAATGATAGTGGTGAACCGCACACTAACTACGATGGTATGAAAAGATTCCGGATGGGT ACAAAAAGGAATAAAAAAGGAAACCCAATAATGGGAAGATCTAAGACCTTGAAATGGGCT AGGAAGAACATTCCAAACCCATTTGAAGACTTTACGAAGGATGATATTGACCCTGGTGCC ATAAATCGTGCGCAAGAACTAAGGACTGTGTATTACAATATGCCCTTACCAAAAGATATG ATAGATGAAGAAGGCAACCCCATTATGCAGTATCCTCGTAATAAAATCAGAACTACCAAA TATACCCCGTTAACATTCTTACCAAAAAACATTCTATTTCAGTTTCATAATTTCGCCAAC GTTTATTTCTTAGTTTTAATAATTTTAGGTGCATTTCAAATTTTTGGTGTCACGAACCCT GGTTTAAGTGCGGTTCCGTTAGTTGTCATTGTTATAATCACTGCGATCAAGGATGCTATT GAAGACTCAAGAAGAACCGTCCTAGATTTAGAGGTGAATAATACCAAGACACATATTTTA GAAGGTGTTGAGAACGAAAATGTCTCAACCGATAATATTTCATTGTGGAGAAGGTTTAAG AAAGCAAACTCTAGACTCCTTTTCAAGTTTATACAATATTGTAAAGAACATTTAACTGAA GAGGGCAAAAAGAAGAGAATGCAGAGAAAGCGTCATGAACTAAGAGTACAAAAAACTGTT GGAACGAGTGGACCCAGATCCTCTCTAGACTCCATTGACAGTTACAGAGTATCTGCAGAT TATGGCCGTCCTTCATTGGATTATGATAATTTGGAACAAGGGGCCGGTGAAGCCAATATA GTTGATAGATCCCTACCACCCAGAACTGATTGTAAATTTGCCAAGAATTATTGGAAAGGT GTCAAGGTTGGTGATATTGTTCGTATCCATAACAATGATGAAATCCCTGCTGACATTATC TTACTTTCAACGTCGGATACAGATGGTGCCTGCTACGTAGAAACTAAAAATTTGGATGGT GAAACAAACTTAAAGGTACGCCAATCCTTGAAGTGTACCAATACTATAAGAACGTCAAAA GATATTGCTAGAACAAAATTCTGGATCGAAAGTGAAGGGCCCCACTCAAATTTGTACACT TATCAAGGTAATATGAAATGGAGAAATCTTGCAGATGGCGAGATACGAAATGAGCCAATT ACTATTAATAACGTTCTGCTTCGTGGTTGCACTCTTAGAAATACTAAGTGGGCAATGGGT GTTGTAATGTTTACCGGTGGTGACACAAAAATCATGTTAAACTCTGGCATTACACCCACC AAGAAGTCCAGGATTTCAAGAGAACTAAACTTCTCTGTGGTGATAAATTTCGTGCTACTT TTCATCTTATGTTTTGTTTCTGGTATTGCAAATGGTGTATATTACGACAAAAAAGGCAGA TCACGGTTTTCCTATGAATTTGGAACAATTGCTGGGTCAGCGGCGACAAACGGTTTCGTC TCATTTTGGGTTGCTGTTATTCTTTACCAATCTTTAGTCCCAATCTCTTTGTACATTTCT GTGGAGATCATCAAAACTGCACAAGCGGCCTTCATTTACGGAGACGTTTTACTCTACAAT GCCAAATTGGATTATCCTTGTACACCAAAATCCTGGAATATTTCTGACGATTTAGGTCAA GTGGAATATATATTTTCTGATAAAACAGGTACATTAACTCAAAATGTAATGGAGTTTAAA AAATGTACTATCAATGGTGTTTCATATGGAAGAGCTTACACCGAAGCGTTAGCGGGATTA AGAAAAAGACAAGGTATCGACGTCGAAACCGAAGGTAGGCGTGAGAAGGCCGAAATTGCC AAGGATAGGGATACAATGATCGATGAACTGAGAGCTTTATCAGGAAATTCACAATTTTAT CCCGAAGAAGTTACCTTTGTATCAAAGGAGTTCGTTCGCGATTTGAAAGGGGCTAGTGGT GAAGTACAACAAAGATGCTGTGAGCACTTTATGTTAGCATTAGCTTTATGTCACTCCGTC CTTGTAGAAGCAAACCCGGACAATCCAAAAAAACTGGATTTAAAAGCTCAATCTCCAGAC GAAGCAGCGTTAGTAGCCACAGCTAGAGATGTAGGATTCAGTTTTGTTGGAAAAACCAAA AAAGGGTTGATAATTGAAATGCAAGGTATTCAAAAAGAATTTGAGATTCTAAATATTTTG GAGTTTAATTCATCGAGAAAAAGAATGTCCTGTATTGTCAAAATTCCGGGTCTGAACCCA GGGGACGAGCCTAGAGCACTTTTAATTTGTAAAGGTGCAGATTCCATTATATACTCAAGA TTAAGTCGCCAGAGTGGTAGCAATAGTGAAGCTATTTTGGAAAAAACTGCTTTGCATTTA GAGCAGTACGCTACTGAAGGTTTAAGAACGTTATGCATAGCTCAAAGGGAGTTAAGTTGG TCTGAATACGAAAAATGGAACGAAAAGTACGATATTGCTGCTGCATCATTAGCAAACAGA GAGGATGAATTAGAAGTTGTTGCAGATTCCATTGAACGCGAATTGATATTATTGGGCGGT ACAGCTATCGAAGATCGTTTACAAGATGGTGTTCCGGATTGTATAGAGTTGCTTGCAGAG GCAGGAATTAAATTATGGGTTCTTACGGGTGATAAAGTTGAAACAGCTATAAACATTGGA TTTTCTTGCAACCTTTTGAATAATGAAATGGAATTGTTAGTTATTAAAACAACTGGTGAT GACGTGAAAGAATTCGGATCAGAACCAAGTGAAATTGTGGATGCTTTACTCTCCAAATAC TTGAAGGAGTATTTCAACTTAACCGGTTCAGAAGAAGAGATTTTTGAAGCTAAAAAGGAT CACGAATTTCCGAAGGGTAATTACGCCATCGTTATCGATGGTGATGCTTTGAAATTGGCG CTATATGGTGAAGATATAAGAAGAAAGTTTCTTTTACTCTGCAAGAATTGTAGAGCTGTC TTATGCTGTAGAGTATCTCCATCTCAAAAAGCAGCCGTTGTTAAGTTAGTCAAGGATTCT CTAGATGTCATGACACTAGCCATCGGCGATGGTTCCAACGACGTCGCCATGATTCAATCT GCGGATGTTGGTATTGGTATTGCCGGTGAAGAAGGTCGACAAGCTGTTATGTGCTCAGAC TACGCCATTGGACAATTCAGATATTTGGCTAGATTGGTCCTCGTTCACGGTAGGTGGTCT TATAAGAGACTGGCAGAAATGATTCCTGAGTTTTTTTACAAAAACATGATTTTTGCTTTG GCATTGTTCTGGTATGGTATCTATAATGATTTTGATGGGTCTTACCTGTATGAATACACA TACATGATGTTCTATAATTTGGCATTTACATCCCTACCAGTCATCTTCTTAGGTATTCTG GACCAGGACGTGAATGACACAATATCATTAGTGGTCCCTCAGTTATACCGTGTTGGAATT TTAAGGAAAGAATGGAACCAAAGAAAGTTCTTATGGTATATGTTGGACGGATTATATCAG TCTATTATCTGTTTTTTTTTCCCTTACTTGGTTTATCACAAAAATATGATAGTTACGAGC AATGGGTTAGGATTGGATCATCGTTATTTTGTCGGTGTTTATGTGACCACTATTGCTGTA ATTTCGTGTAACACATATGTTTTATTACACCAATATAGATGGGATTGGTTTAGTGGATTA TTCATTGCACTGTCTTGCCTCGTAGTTTTTGCATGGACGGGAATATGGTCTTCTGCGATA GCTAGTAGAGAATTTTTCAAAGCTGCAGCGCGTATCTATGGAGCACCTTCATTTTGGGCT GTTTTCTTTGTTGCCGTGCTGTTCTGCTTACTGCCCCGTTTCACATATGACAGTTTTCAA AAATTTTTCTACCCAACTGATGTGGAAATAGTAAGAGAGATGTGGCAACATGGCCATTTC GATCATTATCCTCCTGGTTACGATCCAACAGATCCCAACAGACCCAAGGTCACAAAAGCT GGGCAACACGGAGAGAAGATTATAGAAGGCATAGCACTTTCCGACAACTTAGGAGGATCG AACTATTCAAGGGATAGTGTTGTCACAGAGGAGATTCCAATGACTTTCATGCACGGTGAA GATGGAAGTCCTAGCGGATATCAGAAGCAAGAAACCTGGATGACTTCACCCAAGGAAACA CAAGATCTTCTTCAATCGCCCCAATTTCAGCAGGCCCAAACGTTCGGAAGAGGCCCATCA ACCAACGTCAGGTCATCCTTGGACCGCACAAGAGAACAGATGATCGCCACTAACCAACTA GATAACCGCTACTCTGTGGAAAGAGCGAGAACATCTCTAGATCTACCAGGCGTAACTAAC GCAGCCTCCTTAATCGGAACACAACAGAACAATTAA
Protein Properties
Pfam Domain Function
Protein Residues1571
Protein Molecular Weight177796.0
Protein Theoretical pI5.87
Signalling Regions
  • None
Transmembrane Regions
  • 215-235
  • 240-260
  • 554-574
  • 595-615
  • 1189-1209
  • 1220-1240
  • 1271-1291
  • 1308-1328
  • 1335-1355
  • 1376-1396
Protein Sequence>Probable phospholipid-transporting ATPase DNF1 MSGTFHGDGHAPMSPFEDTFQFEDNSSNEDTHIAPTHFDDGATSNKYSRPQVSFNDETPK NKREDAEEFTFNDDTEYDNHSFQPTPKLNNGSGTFDDVELDNDSGEPHTNYDGMKRFRMG TKRNKKGNPIMGRSKTLKWARKNIPNPFEDFTKDDIDPGAINRAQELRTVYYNMPLPKDM IDEEGNPIMQYPRNKIRTTKYTPLTFLPKNILFQFHNFANVYFLVLIILGAFQIFGVTNP GLSAVPLVVIVIITAIKDAIEDSRRTVLDLEVNNTKTHILEGVENENVSTDNISLWRRFK KANSRLLFKFIQYCKEHLTEEGKKKRMQRKRHELRVQKTVGTSGPRSSLDSIDSYRVSAD YGRPSLDYDNLEQGAGEANIVDRSLPPRTDCKFAKNYWKGVKVGDIVRIHNNDEIPADII LLSTSDTDGACYVETKNLDGETNLKVRQSLKCTNTIRTSKDIARTKFWIESEGPHSNLYT YQGNMKWRNLADGEIRNEPITINNVLLRGCTLRNTKWAMGVVMFTGGDTKIMLNSGITPT KKSRISRELNFSVVINFVLLFILCFVSGIANGVYYDKKGRSRFSYEFGTIAGSAATNGFV SFWVAVILYQSLVPISLYISVEIIKTAQAAFIYGDVLLYNAKLDYPCTPKSWNISDDLGQ VEYIFSDKTGTLTQNVMEFKKCTINGVSYGRAYTEALAGLRKRQGIDVETEGRREKAEIA KDRDTMIDELRALSGNSQFYPEEVTFVSKEFVRDLKGASGEVQQRCCEHFMLALALCHSV LVEANPDNPKKLDLKAQSPDEAALVATARDVGFSFVGKTKKGLIIEMQGIQKEFEILNIL EFNSSRKRMSCIVKIPGLNPGDEPRALLICKGADSIIYSRLSRQSGSNSEAILEKTALHL EQYATEGLRTLCIAQRELSWSEYEKWNEKYDIAAASLANREDELEVVADSIERELILLGG TAIEDRLQDGVPDCIELLAEAGIKLWVLTGDKVETAINIGFSCNLLNNEMELLVIKTTGD DVKEFGSEPSEIVDALLSKYLKEYFNLTGSEEEIFEAKKDHEFPKGNYAIVIDGDALKLA LYGEDIRRKFLLLCKNCRAVLCCRVSPSQKAAVVKLVKDSLDVMTLAIGDGSNDVAMIQS ADVGIGIAGEEGRQAVMCSDYAIGQFRYLARLVLVHGRWSYKRLAEMIPEFFYKNMIFAL ALFWYGIYNDFDGSYLYEYTYMMFYNLAFTSLPVIFLGILDQDVNDTISLVVPQLYRVGI LRKEWNQRKFLWYMLDGLYQSIICFFFPYLVYHKNMIVTSNGLGLDHRYFVGVYVTTIAV ISCNTYVLLHQYRWDWFSGLFIALSCLVVFAWTGIWSSAIASREFFKAAARIYGAPSFWA VFFVAVLFCLLPRFTYDSFQKFFYPTDVEIVREMWQHGHFDHYPPGYDPTDPNRPKVTKA GQHGEKIIEGIALSDNLGGSNYSRDSVVTEEIPMTFMHGEDGSPSGYQKQETWMTSPKET QDLLQSPQFQQAQTFGRGPSTNVRSSLDRTREQMIATNQLDNRYSVERARTSLDLPGVTN AASLIGTQQNN
References
External Links
ResourceLink
Saccharomyces Genome Database DNF1
Uniprot IDP32660
Uniprot NameATC5_YEAST
GenBank Gene IDU18922
Genebank Protein ID603407
General Reference
  • Dietrich, F. S., Mulligan, J., Hennessy, K., Yelton, M. A., Allen, E., Araujo, R., Aviles, E., Berno, A., Brennan, T., Carpenter, J., Chen, E., Cherry, J. M., Chung, E., Duncan, M., Guzman, E., Hartzell, G., Hunicke-Smith, S., Hyman, R. W., Kayser, A., Komp, C., Lashkari, D., Lew, H., Lin, D., Mosedale, D., Davis, R. W., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Nature 387:78-81.9169868
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956