Diphthine synthase (P32469)

Identification
NameDiphthine synthase
Synonyms
  • Diphthamide biosynthesis methyltransferase
Gene NameDPH5
Enzyme Class
Biological Properties
General FunctionInvolved in methyltransferase activity
Specific FunctionRequired for the methylation step in diphthamide biosynthesis
Cellular LocationCytoplasm
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG Reactions
2-(3-Carboxy-3-aminopropyl)-L-histidine + S-Adenosylmethionine → S-Adenosylhomocysteine + hydron + 2-(3-Carboxy-3-(methylammonio)propyl)-L-histidine
Metabolites
YMDB IDNameView
YMDB000172-(3-Carboxy-3-aminopropyl)-L-histidine Show
YMDB00198S-AdenosylhomocysteineShow
YMDB00297S-AdenosylmethionineShow
YMDB003022-(3-Carboxy-3-(methylammonio)propyl)-L-histidineShow
YMDB00438S-Adenosyl-L-methionineShow
YMDB006732-[3-carboxy-3-(methylammonio)propyl]-L-histidineShow
YMDB00862hydronShow
GO Classification
Component
Not Available
Function
methyltransferase activity
S-adenosylmethionine-dependent methyltransferase activity
diphthine synthase activity
catalytic activity
transferase activity
transferase activity, transferring one-carbon groups
Process
peptidyl-amino acid modification
peptidyl-histidine modification
peptidyl-diphthamide metabolic process
peptidyl-diphthamide biosynthetic process from peptidyl-histidine
metabolic process
macromolecule metabolic process
macromolecule modification
protein modification process
Gene Properties
Chromosome Locationchromosome 12
LocusYLR172C
Gene Sequence>903 bp ATGCTTTATTTGATCGGACTTGGTCTCTCGTACAAATCAGACATTACCGTTCGTGGTTTG GAAGCTATTAAGAAATGTTCTAGAGTTTATCTAGAACACTATACCAGTATCCTAATGGCT GCAAGCCAAGAAGAGTTAGAATCTTACTATGGTAAAGAGATCATCTTGGCTGATAGGGAA TTAGTTGAGACTGGTTCTAAGCAGATCCTAAATAACGCCGATAAGGAAGACGTTGCTTTC TTGGTCGTGGGCGATCCATTTGGTGCCACCACACACACAGATTTAGTTCTCAGAGCTAAA CGTGAGGCAATTCCCGTCGAAATTATTCATAATGCGTCCGTTATGAATGCAGTTGGGGCA TGTGGCCTACAACTATACAATTTCGGTCAAACCGTTTCCATGGTTTTCTTTACCGATAAT TGGAGACCAGACTCATGGTACGACAAGATCTGGGAAAATAGAAAAATTGGCCTTCATACT TTAGTGTTATTGGACATCAAAGTTAAGGAACAAAGCATTGAAAATATGGCCCGTGGCAGA CTAATCTACGAACCACCAAGATACATGTCTATCGCTCAATGTTGTGAACAATTATTAGAA ATTGAAGAGAAAAGAGGTACAAAGGCATACACTCCTGATACTCCAGCAGTCGCAATTAGT AGATTAGGCTCGAGCTCCCAAAGCTTTAAGTCTGGTACCATAAGTGAGTTAGCCAATTAC GATTCAGGAGAGCCACTTCATTCGCTTGTCATCCTCGGCAGACAATGTCATGAATTGGAG CTGGAATACCTGCTAGAGTTTGCCGACGACAAAGAAAAGTTTGGGAAAGATGTGGCAAAT GACCAAGAGTACTTCAAACTTGCGGCATGGGTCCCACCCACAGAAGACGACAGCGACGAG TAA
Protein Properties
Pfam Domain Function
Protein Residues300
Protein Molecular Weight33847.0
Protein Theoretical pI4.56
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Diphthine synthase MLYLIGLGLSYKSDITVRGLEAIKKCSRVYLEHYTSILMAASQEELESYYGKEIILADRE LVETGSKQILNNADKEDVAFLVVGDPFGATTHTDLVLRAKREAIPVEIIHNASVMNAVGA CGLQLYNFGQTVSMVFFTDNWRPDSWYDKIWENRKIGLHTLVLLDIKVKEQSIENMARGR LIYEPPRYMSIAQCCEQLLEIEEKRGTKAYTPDTPAVAISRLGSSSQSFKSGTISELANY DSGEPLHSLVILGRQCHELELEYLLEFADDKEKFGKDVANDQEYFKPAAWVPPTEDDSDE
References
External Links
ResourceLink
Saccharomyces Genome Database DPH5
Uniprot IDP32469
Uniprot NameDPH5_YEAST
GenBank Gene IDAY557947
Genebank Protein ID45269786
General Reference
  • Mattheakis, L. C., Shen, W. H., Collier, R. J. (1992). "DPH5, a methyltransferase gene required for diphthamide biosynthesis in Saccharomyces cerevisiae." Mol Cell Biol 12:4026-4037.1508200
  • Johnston, M., Hillier, L., Riles, L., Albermann, K., Andre, B., Ansorge, W., Benes, V., Bruckner, M., Delius, H., Dubois, E., Dusterhoft, A., Entian, K. D., Floeth, M., Goffeau, A., Hebling, U., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hilger, F., Kleine, K., Kotter, P., Louis, E. J., Messenguy, F., Mewes, H. W., Hoheisel, J. D., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Nature 387:87-90.9169871
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Nakai, M., Takada, T., Endo, T. (1993). "Cloning of the YAP19 gene encoding a putative yeast homolog of AP19, the mammalian small chain of the clathrin-assembly proteins." Biochim Biophys Acta 1174:282-284.8373805
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956