Phosphoinositide phosphatase SAC1 (P32368)

Identification
NamePhosphoinositide phosphatase SAC1
Synonyms
  • Recessive suppressor of secretory defect
Gene NameSAC1
Enzyme Class
Biological Properties
General FunctionInvolved in phosphoric ester hydrolase activity
Specific FunctionPhosphoinositide phosphatase that hydrolyzes PtdIns(3)P and PtdIns(4)P. Has low activity towards PtdIns(3,5)P2. May be involved in the coordination of the activities of the secretory pathway and the actin cytoskeleton
Cellular LocationEndoplasmic reticulum membrane; Single-pass membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG Reactions
water + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate ↔ phosphate + 1-phosphatidyl-1D-myo-inositol 3-phosphate + hydron
1-phosphatidyl-1D-myo-inositol 3-phosphate + water ↔ phosphate + 1-Phosphatidyl-D-myo-inositol + hydron
1-phosphatidyl-1D-myo-inositol 4-phosphate + water ↔ phosphate + 1-Phosphatidyl-D-myo-inositol + hydron
Metabolites
YMDB IDNameView
YMDB00862hydronShow
YMDB00890waterShow
GO ClassificationNot Available
Gene Properties
Chromosome Locationchromosome 11
LocusYKL212W
Gene Sequence>1872 bp ATGACAGGTCCAATAGTGTACGTTCAAAATGCGGACGGTATCTTCTTCAAGCTTGCTGAG GGCAAAGGAACTAACGATGCTGTTATTCACTTGGCCAATCAAGATCAAGGTGTTCGGGTC CTTGGAGCAGAGGAATTTCCTGTGCAAGGTGAAGTAGTAAAGATTGCGTCCTTGATGGGG TTCATTAAGCTAAAGTTGAACAGGTATGCCATTATCGCAAATACTGTGGAAGAGACCGGT AGATTCAATGGCCACGTTTTCTATAGAGTGTTGCAACATTCTATCGTATCTACCAAGTTT AACTCGAGAATCGATTCTGAAGAAGCCGAATATATCAAGCTACTGGAGTTGCATTTGAAA AATTCCACCTTTTATTTTTCATACACATATGATTTAACAAATTCCTTACAAAGAAATGAA AAGGTTGGTCCTGCAGCCTCCTGGAAAACCGCTGATGAACGATTCTTTTGGAACCATTAC TTAACTGAAGATTTGAGAAACTTTGCTCATCAAGATCCTAGAATTGACTCCTTTATACAA CCTGTTATCTATGGGTATGCCAAGACAGTGGACGCCGTTTTGAATGCCACCCCTATCGTT CTTGGTTTGATTACCAGACGTAGTATATTTAGGGCGGGCACAAGATACTTCCGTCGTGGT GTTGACAAAGACGGTAACGTTGGCAATTTCAATGAAACTGAGCAAATTTTACTCGCTGAG AATCCAGAGAGTGAAAAAATACACGTTTTCTCCTTCTTACAGACAAGAGGATCTGTGCCA ATATACTGGGCTGAAATCAACAACTTGAAGTACAAGCCAAATCTTGTTCTTGGAGAAAAC TCATTAGATGCGACAAAAAAGCATTTTGACCAGCAAAAGGAGTTATATGGCGACAACTAC TTGGTTAACCTAGTCAACCAAAAGGGCCACGAACTACCCGTGAAAGAGGGCTATGAATCA GTCGTGCACGCGCTAAACGATCCGAAGATTCACTACGTGTATTTTGACTTCCACCATGAA TGTCGTAAGATGCAATGGCATAGAGTGAAATTGTTAATTGATCACCTGGAGAAATTAGGT TTATCTAACGAAGATTTCTTCCACAAGGTCATAGACTCTAATGGTAACACCGTTGAAATT GTTAATGAGCAACATTCCGTTGTAAGAACAAACTGTATGGATTGTTTGGACAGAACAAAT GTCGTTCAATCTGTTTTAGCCCAGTGGGTTTTGCAAAAGGAGTTTGAAAGTGCCGATGTC GTTGCTACTGGAAGCACTTGGGAAGACAACGCTCCATTGTTAACTTCTTACCAAAACTTA TGGGCTGATAATGCAGATGCAGTTAGTGTGGCATATTCGGGCACTGGAGCTTTGAAGACC GATTTCACAAGAACCGGTAAGCGTACACGTCTAGGTGCATTCAACGATTTTTTGAATTCA GCATCACGTTATTACCAGAACAATTGGACTGATGGTCCAAGACAGGATTCATACGATTTA TTCCTTGGTGGATTTAGACCACATACCGCTTCTATCAAGTCGCCATTCCCCGACAGAAGA CCAGTGTATATTCAGCTGATCCCAATGATCATTTGCGCAGCCTTGACCGTTTTAGGTGCT ACGATATTTTTTCCTAAAGATAGATTTACCAGCAGTAAGAATTTGCTGTATTTTGCAGGT GCGTCGATTGTCTTGGCGCTTTCAACCAAATTCATGTTTAAGAACGGTATTCAGTTTGTC AACTGGCCTAAGTTAGTAGACGTTGGGTTCTTGGTCGTTCATCAAACGCATGATAAAGAA CAACAATTCAAAGGTTTGAAATATGCACAGAGTCCTAAATTTTCCAAGCCGGATCCTTTA AAAAGAGATTAA
Protein Properties
Pfam Domain Function
Protein Residues623
Protein Molecular Weight71124.10156
Protein Theoretical pI7.8
Signalling Regions
  • None
Transmembrane Regions
  • 522-544
Protein Sequence>Phosphoinositide phosphatase SAC1 MTGPIVYVQNADGIFFKLAEGKGTNDAVIHLANQDQGVRVLGAEEFPVQGEVVKIASLMG FIKLKLNRYAIIANTVEETGRFNGHVFYRVLQHSIVSTKFNSRIDSEEAEYIKLLELHLK NSTFYFSYTYDLTNSLQRNEKVGPAASWKTADERFFWNHYLTEDLRNFAHQDPRIDSFIQ PVIYGYAKTVDAVLNATPIVLGLITRRSIFRAGTRYFRRGVDKDGNVGNFNETEQILLAE NPESEKIHVFSFLQTRGSVPIYWAEINNLKYKPNLVLGENSLDATKKHFDQQKELYGDNY LVNLVNQKGHELPVKEGYESVVHALNDPKIHYVYFDFHHECRKMQWHRVKLLIDHLEKLG LSNEDFFHKVIDSNGNTVEIVNEQHSVVRTNCMDCLDRTNVVQSVLAQWVLQKEFESADV VATGSTWEDNAPLLTSYQNLWADNADAVSVAYSGTGALKTDFTRTGKRTRLGAFNDFLNS ASRYYQNNWTDGPRQDSYDLFLGGFRPHTASIKSPFPDRRPVYIQLIPMIICAALTVLGA TIFFPKDRFTSSKNLLYFAGASIVLALSTKFMFKNGIQFVNWPKLVDVGFLVVHQTHDKE QQFKGLKYAQSPKFSKPDPLKRD
References
External Links
ResourceLink
Saccharomyces Genome Database SAC1
Uniprot IDP32368
Uniprot NameSAC1_YEAST
GenBank Gene IDX51672
Genebank Protein ID4388552
General Reference
  • Cleves, A. E., Novick, P. J., Bankaitis, V. A. (1989). "Mutations in the SAC1 gene suppress defects in yeast Golgi and yeast actin function." J Cell Biol 109:2939-2950.2687291
  • Tzermia, M., Horaitis, O., Alexandraki, D. (1994). "The complete sequencing of a 24.6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases, membrane transporters, hydantoinases and the phospholipase A2-activating protein." Yeast 10:663-679.7941750
  • Dujon, B., Alexandraki, D., Andre, B., Ansorge, W., Baladron, V., Ballesta, J. P., Banrevi, A., Bolle, P. A., Bolotin-Fukuhara, M., Bossier, P., et, a. l. .. (1994). "Complete DNA sequence of yeast chromosome XI." Nature 369:371-378.8196765
  • Hughes, W. E., Woscholski, R., Cooke, F. T., Patrick, R. S., Dove, S. K., McDonald, N. Q., Parker, P. J. (2000). "SAC1 encodes a regulated lipid phosphoinositide phosphatase, defects in which can be suppressed by the homologous Inp52p and Inp53p phosphatases." J Biol Chem 275:801-808.10625610
  • Foti, M., Audhya, A., Emr, S. D. (2001). "Sac1 lipid phosphatase and Stt4 phosphatidylinositol 4-kinase regulate a pool of phosphatidylinositol 4-phosphate that functions in the control of the actin cytoskeleton and vacuole morphology." Mol Biol Cell 12:2396-2411.11514624
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Kim, H., Melen, K., Osterberg, M., von Heijne, G. (2006). "A global topology map of the Saccharomyces cerevisiae membrane proteome." Proc Natl Acad Sci U S A 103:11142-11147.16847258
  • Breslow, D. K., Collins, S. R., Bodenmiller, B., Aebersold, R., Simons, K., Shevchenko, A., Ejsing, C. S., Weissman, J. S. (2010). "Orm family proteins mediate sphingolipid homeostasis." Nature 463:1048-1053.20182505