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Identification
NameC-8 sterol isomerase
Synonyms
  • Delta-8--delta-7 sterol isomerase
Gene NameERG2
Enzyme Class
Biological Properties
General FunctionInvolved in C-8 sterol isomerase activity
Specific FunctionCatalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols
Cellular LocationEndoplasmic reticulum membrane; Single-pass membrane protein (Potential)
SMPDB Pathways
Steroid biosynthesisPW002482 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Steroid biosynthesisec00100 Map00100
SMPDB Reactions
24-methylidene-5alpha-cholest-8-en-3beta-olepisterol
zymosterol ↔ 5a-cholesta-7,24-dien-3B-ol
KEGG Reactions
24-methylidene-5alpha-cholest-8-en-3beta-olepisterol
Metabolites
YMDB IDNameView
YMDB00540episterolShow
YMDB0054524-methylidene-5alpha-cholest-8-en-3beta-olShow
YMDB00548zymosterolShow
GO Classification
Component
organelle
membrane-bounded organelle
intracellular membrane-bounded organelle
endoplasmic reticulum
Function
isomerase activity
intramolecular oxidoreductase activity
intramolecular oxidoreductase activity, transposing C=C bonds
C-8 sterol isomerase activity
catalytic activity
Process
sterol metabolic process
ergosterol metabolic process
ergosterol biosynthetic process
metabolic process
small molecule metabolic process
alcohol metabolic process
Gene Properties
Chromosome Locationchromosome 13
LocusYMR202W
Gene Sequence>669 bp ATGAAGTTTTTCCCACTCCTTTTGTTGATTGGTGTTGTAGGCTACATTATGAACGTATTG TTCACTACCTGGTTGCCAACCAATTACATGTTCGATCCAAAAACTTTGAACGAAATATGT AACTCGGTGATTAGCAAACACAACGCAGCAGAAGGTTTATCCACTGAAGACCTGTTACAG GATGTCAGAGACGCACTTGCCTCTCATTACGGGGACGAATACATCAACAGGTACGTCAAA GAAGAATGGGTCTTCAACAATGCTGGTGGTGCGATGGGCCAAATGATCATCCTACACGCT TCCGTATCCGAGTACTTAATTCTATTCGGAACCGCTGTTGGTACTGAAGGGCACACAGGT GTTCACTTTGCTGACGACTATTTTACCATCTTACATGGTACGCAAATCGCAGCATTGCCA TATGCCACTGAAGCCGAAGTTTACACTCCTGGTATGACTCATCACTTGAAGAAGGGATAC GCCAAGCAATACAGCATGCCAGGTGGTTCCTTTGCCCTTGAATTGGCTCAAGGCTGGATT CCATGTATGTTGCCATTCGGGTTTTTGGACACTTTCTCCAGTACTCTTGATTTATACACT CTATATAGAACTGTCTACCTGACTGCCAGGGACATGGGTAAGAACTTGTTGCAAAACAAA AAGTTCTAA
Protein Properties
Pfam Domain Function
Protein Residues222
Protein Molecular Weight24895.40039
Protein Theoretical pI5.8
Signalling Regions
  • None
Transmembrane Regions
  • 3-23
Protein Sequence>C-8 sterol isomerase MKFFPLLLLIGVVGYIMNVLFTTWLPTNYMFDPKTLNEICNSVISKHNAAEGLSTEDLLQ DVRDALASHYGDEYINRYVKEEWVFNNAGGAMGQMIILHASVSEYLILFGTAVGTEGHTG VHFADDYFTILHGTQIAALPYATEAEVYTPGMTHHLKKGYAKQYSMPGGSFALELAQGWI PCMLPFGFLDTFSSTLDLYTLYRTVYLTARDMGKNLLQNKKF
References
External Links
ResourceLink
Saccharomyces Genome Database ERG2
Uniprot IDP32352
Uniprot NameERG2_YEAST
GenBank Gene IDAY558122
Genebank Protein ID45270134
General Reference
  • Arthington, B. A., Hoskins, J., Skatrud, P. L., Bard, M. (1991). "Nucleotide sequence of the gene encoding yeast C-8 sterol isomerase." Gene 107:173-174.1743517
  • Bowman, S., Churcher, C., Badcock, K., Brown, D., Chillingworth, T., Connor, R., Dedman, K., Devlin, K., Gentles, S., Hamlin, N., Hunt, S., Jagels, K., Lye, G., Moule, S., Odell, C., Pearson, D., Rajandream, M., Rice, P., Skelton, J., Walsh, S., Whitehead, S., Barrell, B. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Nature 387:90-93.9169872
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Natter, K., Leitner, P., Faschinger, A., Wolinski, H., McCraith, S., Fields, S., Kohlwein, S. D. (2005). "The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopy." Mol Cell Proteomics 4:662-672.15716577