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V-type proton ATPase subunit C (P31412)

Identification
NameV-type proton ATPase subunit C
Synonyms
  • V-ATPase subunit C
  • V-ATPase 42 kDa subunit
  • Vacuolar proton pump subunit C
Gene NameVMA5
Enzyme ClassNot Available
Biological Properties
General FunctionInvolved in ATP binding
Specific FunctionSubunit of the peripheral V1 complex of vacuolar ATPase. Subunit C acts as a flexible stator that holds together the catalytic and the membrane sectors of the enzyme. Reversibly leaves the enzyme after glucose depletion, causing the catalytic subcomplex V1 to detach from the V0 section. Binds ATP and is likely to have a specific function in the catalytic activity of the catalytic sector. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells
Cellular LocationVacuole membrane; Peripheral membrane protein
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
GO Classification
Component
proton-transporting two-sector ATPase complex, catalytic domain
proton-transporting V-type ATPase, V1 domain
macromolecular complex
protein complex
Function
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
hydrolase activity
hydrolase activity, acting on acid anhydrides
catalytic activity
Process
nitrogen compound metabolic process
cellular nitrogen compound metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleoside phosphate metabolic process
nucleotide metabolic process
purine nucleotide metabolic process
purine nucleotide biosynthetic process
purine nucleoside triphosphate biosynthetic process
purine ribonucleoside triphosphate biosynthetic process
ATP biosynthetic process
ATP synthesis coupled proton transport
metabolic process
Gene Properties
Chromosome LocationNot Available
Locus
Gene Sequence>1179 bp ATGGCTACTGCGTTATATACTGCAAACGATTTCATATTAATCTCGTTGCCTCAAAATGCT CAACCTGTTACCGCCCCTGGCTCCAAAACTGATTCATGGTTCAATGAGACCTTAATCGGG GGTAGGGCATTTGTTTCTGATTTCAAAATTCCCGAATTCAAAATTGGCTCTTTAGACACA TTGATAGTTGAATCGGAAGAACTGTCTAAAGTAGATAACCAAATTGGGGCTTCTATTGGC AAAATCATTGAAATTCTTCAAGGCCTCAACGAGACCAGCACCAATGCTTACAGGACTTTA CCTATCAATAATATGCCAGTTCCTGAATACTTGGAAAATTTTCAATGGCAAACCAGAAAG TTCAAGTTAGATAAGTCTATCAAAGATTTGATAACATTGATTTCTAATGAATCTTCTCAA TTAGACGCCGACGTCAGAGCTACTTATGCAAATTACAACAGCGCTAAAACTAACTTGGCT GCTGCTGAGAGAAAGAAGACGGGTGACCTTTCTGTCAGATCCTTGCATGATATTGTCAAG CCCGAAGACTTCGTTCTTAATTCTGAACATTTAACTACTGTTCTAGTAGCAGTTCCCAAA AGTTTAAAATCCGATTTCGAAAAATCGTACGAAACTTTATCCAAGAACGTTGTACCAGCA TCTGCCAGCGTGATTGCAGAGGATGCTGAGTATGTTTTGTTCAATGTTCATTTGTTCAAG AAAAACGTTCAAGAATTCACAACAGCTGCTAGAGAGAAGAAATTCATTCCTCGTGAATTT AACTACTCGGAGGAATTAATTGACCAGTTGAAAAAAGAGCATGACTCTGCTGCCAGTTTA GAACAATCTTTGCGCGTCCAGTTGGTAAGATTGGCCAAGACAGCTTATGTCGATGTTTTT ATAAATTGGTTCCACATCAAGGCCTTGAGAGTTTACGTGGAATCTGTTTTGCGTTACGGG TTGCCACCTCACTTTAACATCAAGATTATAGCTGTCCCACCAAAGAATCTCTCTAAATGC AAGTCTGAATTAATTGATGCTTTTGGATTTCTTGGTGGCAATGCCTTCATGAAGGATAAA AAAGGGAAAATTAACAAGCAAGATACTTCTCTTCACCAATATGCTTCTCTTGTCGACACA GAGTATGAACCATTTGTGATGTATATAATCAATTTATAA
Protein Properties
Pfam Domain Function
Protein Residues392
Protein Molecular Weight44188.10156
Protein Theoretical pI6.65
PDB Fileshow
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>V-type proton ATPase subunit C MATALYTANDFILISLPQNAQPVTAPGSKTDSWFNETLIGGRAFVSDFKIPEFKIGSLDT LIVESEELSKVDNQIGASIGKIIEILQGLNETSTNAYRTLPINNMPVPEYLENFQWQTRK FKLDKSIKDLITLISNESSQLDADVRATYANYNSAKTNLAAAERKKTGDLSVRSLHDIVK PEDFVLNSEHLTTVLVAVPKSLKSDFEKSYETLSKNVVPASASVIAEDAEYVLFNVHLFK KNVQEFTTAAREKKFIPREFNYSEELIDQLKKEHDSAASLEQSLRVQLVRLAKTAYVDVF INWFHIKALRVYVESVLRYGLPPHFNIKIIAVPPKNLSKCKSELIDAFGFLGGNAFMKDK KGKINKQDTSLHQYASLVDTEYEPFVMYIINL
References
External Links
ResourceLink
Saccharomyces Genome Database VMA5
Uniprot IDP31412
Uniprot NameVATC_YEAST
GenBank Gene IDX75560
Genebank Protein ID414714
PDB ID
1U7L
General Reference
  • Beltran, C., Kopecky, J., Pan, Y. C., Nelson, H., Nelson, N. (1992). "Cloning and mutational analysis of the gene encoding subunit C of yeast vacuolar H(+)-ATPase." J Biol Chem 267:774-779.1730668
  • Ho, M. N., Hill, K. J., Lindorfer, M. A., Stevens, T. H. (1993). "Isolation of vacuolar membrane H(+)-ATPase-deficient yeast mutants; the VMA5 and VMA4 genes are essential for assembly and activity of the vacuolar H(+)-ATPase." J Biol Chem 268:221-227.8416931
  • James, C. M., Gent, M. E., Indge, K. J., Oliver, S. G. (1994). "Sequence analysis of a 10 kb fragment of yeast chromosome XI identifies the SMY1 locus and reveals sequences related to a pre-mRNA splicing factor and vacuolar ATPase subunit C plus a number of unidentified open reading frames." Yeast 10:247-255.8203165
  • Dujon, B., Alexandraki, D., Andre, B., Ansorge, W., Baladron, V., Ballesta, J. P., Banrevi, A., Bolle, P. A., Bolotin-Fukuhara, M., Bossier, P., et, a. l. .. (1994). "Complete DNA sequence of yeast chromosome XI." Nature 369:371-378.8196765
  • Armbruster, A., Hohn, C., Hermesdorf, A., Schumacher, K., Borsch, M., Gruber, G. (2005). "Evidence for major structural changes in subunit C of the vacuolar ATPase due to nucleotide binding." FEBS Lett 579:1961-1967.15792803
  • Parra, K. J., Keenan, K. L., Kane, P. M. (2000). "The H subunit (Vma13p) of the yeast V-ATPase inhibits the ATPase activity of cytosolic V1 complexes." J Biol Chem 275:21761-21767.10781598
  • Curtis, K. K., Francis, S. A., Oluwatosin, Y., Kane, P. M. (2002). "Mutational analysis of the subunit C (Vma5p) of the yeast vacuolar H+-ATPase." J Biol Chem 277:8979-8988.11777935
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Jones, R. P., Durose, L. J., Findlay, J. B., Harrison, M. A. (2005). "Defined sites of interaction between subunits E (Vma4p), C (Vma5p), and G (Vma10p) within the stator structure of the vacuolar H+-ATPase." Biochemistry 44:3933-3941.15751969
  • Drory, O., Frolow, F., Nelson, N. (2004). "Crystal structure of yeast V-ATPase subunit C reveals its stator function." EMBO Rep 5:1148-1152.15540116