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Identification
NameGlycolipid 2-alpha-mannosyltransferase
Synonyms
  • Alpha-1,2-mannosyltransferase
Gene NameKRE2
Enzyme Class
Biological Properties
General FunctionInvolved in mannosyltransferase activity
Specific FunctionRequired for the attachment of the third mannose residue of O-linked saccharides
Cellular LocationGolgi apparatus membrane; Single-pass type II membrane protein
SMPDB Pathways
N-Glycan biosynthesisPW002501 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
N-Glycan biosynthesisec00510 Map00510
SMPDB ReactionsNot Available
KEGG Reactions
Beta-1,4-D-Mannosylchitobiosyldiphosphodolichol + Guanosine diphosphate mannosealpha-D-mannosyl-beta-D-mannosyldiacetylchitobiosyldiphosphodolichol + hydron + Guanosine diphosphate
Metabolites
YMDB IDNameView
YMDB00202Guanosine diphosphate mannoseShow
YMDB00257Guanosine diphosphateShow
YMDB00581alpha-D-mannosyl-beta-D-mannosyldiacetylchitobiosyldiphosphodolicholShow
YMDB00862hydronShow
YMDB00935beta-D-mannosyldiacetylchitobiosyldiphosphodolicholShow
GO Classification
Component
cell part
membrane
Function
transferase activity, transferring glycosyl groups
transferase activity, transferring hexosyl groups
mannosyltransferase activity
alpha-1,2-mannosyltransferase activity
glycolipid 2-alpha-mannosyltransferase activity
catalytic activity
transferase activity
Process
metabolic process
macromolecule metabolic process
macromolecule modification
protein modification process
protein amino acid glycosylation
Gene Properties
Chromosome Locationchromosome 4
LocusYDR483W
Gene Sequence>1329 bp ATGGCCCTCTTTCTCAGTAAGAGACTGTTGAGATTTACCGTCATTGCAGGTGCGGTTATT GTTCTCCTCCTAACATTGAATTCCAACAGTAGAACTCAGCAATATATTCCGAGTTCCATC TCCGCTGCATTTGATTTTACCTCAGGATCTATATCCCCTGAACAACAAGTCATCTCTGAG GAAAATGATGCTAAAAAATTAGAGCAAAGTGCTCTGAATTCAGAGGCAAGCGAAGACTCC GAAGCCATGGATGAAGAATCCAAGGCTCTGAAAGCTGCCGCTGAAAAGGCAGATGCCCCG ATCGACACTAAAACAACCATGGATTATATCACTCCATCTTTTGCTAACAAAGCTGGTAAG CCAAAAGCTTGTTACGTCACTTTGGTGAGAAACAAGGAGTTGAAAGGTTTGCTAAGCTCC ATTAAATATGTGGAAAACAAAATTAACAAGAAATTCCCATATCCTTGGGTTTTCCTAAAC GATGAACCTTTTACTGAAGAATTCAAGGAAGCAGTCACCAAAGCTGTTTCTTCCGAAGTT AAGTTTGGTATTTTGCCCAAGGAACATTGGTCATATCCTGAATGGATTAATCAAACCAAG GCTGCTGAAATTCGTGCAGATGCTGCCACCAAATACATATACGGTGGCTCCGAATCTTAT AGACACATGTGTCGTTACCAATCTGGGTTTTTCTGGAGACATGAATTATTAGAAGAGTAC GATTGGTACTGGCGTGTGGAACCAGACATCAAGTTATACTGTGATATTAATTACGACGTT TTTAAGTGGATGCAAGAAAACGAAAAAGTTTACGGCTTTACCGTTTCTATTCATGAATAT GAAGTGACGATCCCAACACTATGGCAAACGTCCATGGATTTCATCAAAAAGAACCCCGAA TACTTAGATGAAAACAACCTGATGAGTTTTCTTTCGAACGATAATGGTAAAACATACAAT CTGTGCCATTTCTGGTCAAACTTTGAAATTGCAAACTTGAATTTGTGGAGGTCACCAGCC TACAGAGAGTATTTTGACACTTTGGATCATCAAGGTGGATTTTTCTACGAAAGATGGGGC GATGCTCCCGTTCATTCTATTGCTGCTGCTTTGTTTTTGCCAAAGGATAAAATCCATTAT TTTTCAGACATTGGTTACCATCATCCACCTTATGATAACTGCCCATTGGACAAGGAGGTC TATAACAGTAACAACTGTGAATGTGACCAAGGTAATGATTTCACTTTCCAAGGTTACTCT TGTGGTAAGGAATATTATGATGCTCAAGGGTTGGTAAAGCCAAAAAACTGGAAAAAATTC CGTGAGTAG
Protein Properties
Pfam Domain Function
Protein Residues442
Protein Molecular Weight51386.19922
Protein Theoretical pI5.07
PDB Fileshow
Signalling Regions
  • None
Transmembrane Regions
  • 12-30
Protein Sequence>Glycolipid 2-alpha-mannosyltransferase MALFLSKRLLRFTVIAGAVIVLLLTLNSNSRTQQYIPSSISAAFDFTSGSISPEQQVISE ENDAKKLEQSALNSEASEDSEAMDEESKALKAAAEKADAPIDTKTTMDYITPSFANKAGK PKACYVTLVRNKELKGLLSSIKYVENKINKKFPYPWVFLNDEPFTEEFKEAVTKAVSSEV KFGILPKEHWSYPEWINQTKAAEIRADAATKYIYGGSESYRHMCRYQSGFFWRHELLEEY DWYWRVEPDIKLYCDINYDVFKWMQENEKVYGFTVSIHEYEVTIPTLWQTSMDFIKKNPE YLDENNLMSFLSNDNGKTYNLCHFWSNFEIANLNLWRSPAYREYFDTLDHQGGFFYERWG DAPVHSIAAALFLPKDKIHYFSDIGYHHPPYDNCPLDKEVYNSNNCECDQGNDFTFQGYS CGKEYYDAQGLVKPKNWKKFRE
References
External Links
ResourceLink
Saccharomyces Genome Database KRE2
Uniprot IDP27809
Uniprot NameKRE2_YEAST
GenBank Gene IDM81110
Genebank Protein ID171961
PDB ID
1S4P
General Reference
  • Hausler, A., Robbins, P. W. (1992). "Glycosylation in Saccharomyces cerevisiae: cloning and characterization of an alpha-1,2-mannosyltransferase structural gene." Glycobiology 2:77-84.1550992
  • Hill, K., Boone, C., Goebl, M., Puccia, R., Sdicu, A. M., Bussey, H. (1992). "Yeast KRE2 defines a new gene family encoding probable secretory proteins, and is required for the correct N-glycosylation of proteins." Genetics 130:273-283.1541391
  • Jacq, C., Alt-Morbe, J., Andre, B., Arnold, W., Bahr, A., Ballesta, J. P., Bargues, M., Baron, L., Becker, A., Biteau, N., Blocker, H., Blugeon, C., Boskovic, J., Brandt, P., Bruckner, M., Buitrago, M. J., Coster, F., Delaveau, T., del Rey, F., Dujon, B., Eide, L. G., Garcia-Cantalejo, J. M., Goffeau, A., Gomez-Peris, A., Zaccaria, P., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature 387:75-78.9169867
  • Kolling, R., Lee, A., Chen, E. Y., Botstein, D. (1994). "Nucleotide sequence of the SAC2 gene of Saccharomyces cerevisiae." Yeast 10:1211-1216.7754710
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106