Sterol O-acyltransferase 1 (P25628)

Identification
NameSterol O-acyltransferase 1
Synonyms
  • Sterol-ester synthase 1
Gene NameARE1
Enzyme Class
Biological Properties
General FunctionInvolved in O-acyltransferase activity
Specific FunctionAcyl-CoA + cholesterol = CoA + cholesterol ester
Cellular LocationEndoplasmic reticulum membrane; Multi-pass membrane protein
SMPDB Pathways
Steroid biosynthesisPW002482 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Steroid biosynthesisec00100 Map00100
SMPDB ReactionsNot Available
KEGG Reactions
episterol + oleoyl-CoA ↔ episteryl oleate + Coenzyme A
ergosterol + oleoyl-CoA ↔ ergosteryl oleate + Coenzyme A
24-methylidene-5alpha-cholest-8-en-3beta-ol + oleoyl-CoA ↔ fecosteryl oleate + Coenzyme A
oleoyl-CoA + Lanosterin ↔ Coenzyme A + lanosteryl oleate
zymosterol + oleoyl-CoA ↔ zymosteryl oleate + Coenzyme A
Metabolites
YMDB IDNameView
YMDB00045Coenzyme AShow
YMDB00262LanosterinShow
YMDB00313CholesterolShow
YMDB00539oleoyl-CoAShow
YMDB00540episterolShow
YMDB00541episteryl oleateShow
YMDB00542ergosteryl oleateShow
YMDB00543ergosterolShow
YMDB00544fecosteryl oleateShow
YMDB0054524-methylidene-5alpha-cholest-8-en-3beta-olShow
YMDB00546lanosteryl oleateShow
YMDB00547lanosterolShow
YMDB00548zymosterolShow
YMDB00549zymosteryl oleateShow
YMDB00550episteryl palmitoleateShow
YMDB00551ergosteryl palmitoleateShow
YMDB00552fecosteryl palmitoleateShow
YMDB00553lanosteryl palmitoleateShow
YMDB00554zymosteryl palmitoleateShow
GO ClassificationNot Available
Gene Properties
Chromosome Locationchromosome 3
LocusYCR048W
Gene Sequence>1833 bp ATGACGGAGACTAAGGATTTGTTGCAAGACGAAGAGTTTCTTAAGATCCGCAGACTCAAT TCCGCAGAAGCCAACAAACGGCATTCGGTCACGTACGATAACGTGATCCTGCCACAGGAG TCCATGGAGGTTTCGCCACGGTCGTCTACCACGTCGCTGGTGGAGCCAGTGGAGTCGACT GAAGGAGTGGAGTCGACTGAGGCGGAACGTGTGGCAGGGAAGCAGGAGCAGGAGGAGGAG TACCCTGTGGACGCCCACATGCAAAAGTACCTTTCACACCTGAAGAGCAAGTCTCGGTCG AGGTTCCACCGAAAGGATGCTAGCAAGTATGTGTCGTTTTTTGGGGACGTGAGTTTTGAT CCTCGCCCCACGCTCCTGGACAGCGCCATCAACGTGCCCTTCCAGACGACTTTCAAAGGT CCGGTGCTGGAGAAACAGCTCAAAAATTTACAGTTGACAAAGACCAAGACCAAGGCCACG GTGAAGACTACGGTGAAGACTACGGAGAAAACGGACAAGGCAGATGCCCCCCCAGGAGAA AAACTGGAGTCGAACTTTTCAGGGATCTACGTGTTCGCATGGATGTTCTTGGGCTGGATA GCCATCAGGTGCTGCACAGATTACTATGCGTCGTACGGCAGTGCATGGAATAAGCTGGAA ATCGTGCAGTACATGACAACGGACTTGTTCACGATCGCAATGTTGGACTTGGCAATGTTC CTGTGCACTTTCTTCGTGGTTTTCGTGCACTGGCTGGTGAAAAAGCGGATCATCAACTGG AAGTGGACTGGGTTCGTTGCAGTGAGCATCTTCGAGTTGGCTTTCATCCCCGTGACGTTC CCCATTTACGTCTACTACTTTGATTTCAACTGGGTCACGAGAATCTTCCTGTTCCTGCAC TCCGTGGTGTTTGTTATGAAGAGCCACTCGTTTGCCTTTTACAACGGGTATCTTTGGGAC ATAAAGCAGGAACTCGAGTACTCTTCCAAACAGTTGCAAAAATACAAGGAATCTTTGTCC CCAGAGACCCGCGAGATTCTGCAAAAAAGTTGCGACTTTTGCCTTTTCGAATTGAACTAC CAGACCAAGGATAACGACTTCCCCAACAACATCAGTTGCAGCAATTTCTTCATGTTCTGT TTGTTCCCCGTCCTCGTGTACCAGATCAACTACCCAAGAACGTCGCGCATCAGATGGAGG TATGTGTTGGAGAAGGTGTGCGCCATCATTGGCACCATCTTCCTCATGATGGTCACGGCA CAGTTCTTCATGCACCCGGTGGCCATGCGCTGTATCCAGTTCCACAACACGCCCACCTTC GGCGGCTGGATCCCCGCCACGCAAGAGTGGTTCCACCTGCTCTTCGACATGATTCCGGGC TTCACTGTTCTGTACATGCTCACGTTTTACATGATATGGGACGCTTTATTGAATTGCGTG GCGGAGTTGACCAGGTTTGCGGACAGATATTTCTACGGCGACTGGTGGAATTGCGTTTCG TTTGAAGAGTTTAGCAGAATCTGGAACGTCCCCGTTCACAAATTTTTACTAAGACACGTG TACCACAGCTCCATGGGCGCATTGCATTTGAGCAAGAGCCAAGCTACATTATTTACTTTT TTCTTGAGTGCCGTGTTCCACGAAATGGCCATGTTCGCCATTTTCAGAAGGGTTAGAGGA TATCTGTTCATGTTCCAACTGTCGCAGTTTGTGTGGACTGCTTTGAGCAACACCAAGTTT CTACGGGCAAGACCGCAGTTGTCCAACGTTGTCTTTTCGTTTGGTGTCTGTTCAGGGCCC AGTATCATTATGACGTTGTACCTGACCTTATGA
Protein Properties
Pfam Domain Function
Protein Residues610
Protein Molecular Weight71612.5
Protein Theoretical pI8.38
Signalling Regions
  • None
Transmembrane Regions
  • 182-202
  • 229-249
  • 264-284
  • 371-391
  • 409-429
  • 535-555
  • 590-610
Protein Sequence>Sterol O-acyltransferase 1 MTETKDLLQDEEFLKIRRLNSAEANKRHSVTYDNVILPQESMEVSPRSSTTSLVEPVEST EGVESTEAERVAGKQEQEEEYPVDAHMQKYLSHLKSKSRSRFHRKDASKYVSFFGDVSFD PRPTLLDSAINVPFQTTFKGPVLEKQLKNLQLTKTKTKATVKTTVKTTEKTDKADAPPGE KLESNFSGIYVFAWMFLGWIAIRCCTDYYASYGSAWNKLEIVQYMTTDLFTIAMLDLAMF LCTFFVVFVHWLVKKRIINWKWTGFVAVSIFELAFIPVTFPIYVYYFDFNWVTRIFLFLH SVVFVMKSHSFAFYNGYLWDIKQELEYSSKQLQKYKESLSPETREILQKSCDFCLFELNY QTKDNDFPNNISCSNFFMFCLFPVLVYQINYPRTSRIRWRYVLEKVCAIIGTIFLMMVTA QFFMHPVAMRCIQFHNTPTFGGWIPATQEWFHLLFDMIPGFTVLYMLTFYMIWDALLNCV AELTRFADRYFYGDWWNCVSFEEFSRIWNVPVHKFLLRHVYHSSMGALHLSKSQATLFTF FLSAVFHEMAMFAIFRRVRGYLFMFQLSQFVWTALSNTKFLRARPQLSNVVFSFGVCSGP SIIMTLYLTL
References
External Links
ResourceLink
Saccharomyces Genome Database ARE1
Uniprot IDP25628
Uniprot NameARE1_YEAST
GenBank Gene IDAY692921
Genebank Protein ID51013293
General Reference
  • Oliver, S. G., van der Aart, Q. J., Agostoni-Carbone, M. L., Aigle, M., Alberghina, L., Alexandraki, D., Antoine, G., Anwar, R., Ballesta, J. P., Benit, P., et, a. l. .. (1992). "The complete DNA sequence of yeast chromosome III." Nature 357:38-46.1574125
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Yang, H., Bard, M., Bruner, D. A., Gleeson, A., Deckelbaum, R. J., Aljinovic, G., Pohl, T. M., Rothstein, R., Sturley, S. L. (1996). "Sterol esterification in yeast: a two-gene process." Science 272:1353-1356.8650549
  • Li, X., Gerber, S. A., Rudner, A. D., Beausoleil, S. A., Haas, W., Villen, J., Elias, J. E., Gygi, S. P. (2007). "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." J Proteome Res 6:1190-1197.17330950
  • Chi, A., Huttenhower, C., Geer, L. Y., Coon, J. J., Syka, J. E., Bai, D. L., Shabanowitz, J., Burke, D. J., Troyanskaya, O. G., Hunt, D. F. (2007). "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Proc Natl Acad Sci U S A 104:2193-2198.17287358
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956