You are using an unsupported browser. Please upgrade your browser to a newer version to get the best experience on Yeast Metabolome Database.
Identification
NamePhenylalanyl-tRNA synthetase alpha chain
Synonyms
  • Phenylalanine--tRNA ligase alpha chain
  • PheRS
Gene NameFRS2
Enzyme Class
Biological Properties
General FunctionInvolved in nucleotide binding
Specific FunctionATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
Cellular LocationCytoplasm
SMPDB PathwaysNot Available
KEGG PathwaysNot Available
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00097Adenosine monophosphateShow
YMDB00109Adenosine triphosphateShow
YMDB00219PyrophosphateShow
YMDB00304L-PhenylalanineShow
GO Classification
Component
intracellular part
cytoplasm
cell part
Function
ligase activity
ligase activity, forming aminoacyl-tRNA and related compounds
aminoacyl-tRNA ligase activity
nucleic acid binding
binding
nucleotide binding
RNA binding
tRNA binding
phenylalanine-tRNA ligase activity
nucleoside binding
purine nucleoside binding
catalytic activity
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ligase activity, forming carbon-oxygen bonds
Process
translation
biosynthetic process
cellular macromolecule metabolic process
RNA metabolic process
ncRNA metabolic process
phenylalanyl-tRNA aminoacylation
tRNA metabolic process
tRNA aminoacylation
metabolic process
tRNA aminoacylation for protein translation
macromolecule metabolic process
macromolecule biosynthetic process
cellular macromolecule biosynthetic process
Gene Properties
Chromosome Locationchromosome 6
LocusYFL022C
Gene Sequence>1512 bp ATGTCTGATTTCCAATTAGAAATTCTAAAAAAACTAGATGAATTGGATGAGATCAAGTCC ACACTGGCAACTTTCCCTCAGCACGGCTCTCAAGATGTTCTTTCCGCTTTGAACTCTTTG AAAGCCCACAACAAGTTAGAGTTTTCCAAGGTCGACACGGTTACGTATGACTTGACCAAA GAAGGTGCTCAAATTTTGAATGAAGGTTCGTACGAAATTAAACTAGTCAAGCTCATCCAA GAGTTGGGTCAACTTCAAATCAAAGATGTGATGTCCAAACTGGGTCCTCAAGTTGGTAAG GTCGGTCAGGCTAGAGCTTTCAAGAACGGCTGGATCGCCAAAAACGCCTCAAACGAGCTT GAACTCTCCGCAAAATTGCAAAATACCGATTTAAATGAGCTTACTGATGAAACGCAATCT ATTCTAGCGCAAATCAAGAACAACTCGCATCTGGATAGCATTGACGCCAAGATTTTGAAC GACTTGAAGAAAAGAAAGTTAATTGCTCAAGGTAAAATCACAGATTTCAACGTTACCAAA GGGCCAGAGTTCTCGACCGACCTCACCAAATTGGAAACCGATCTTACCTCCGACATGGTC TCCACCAATGCATACAAGGACTTGAAGTTCAAGCCTTACAATTTCAATTCTCAAGGTGTG CAAATATCTTCAGGTGCTCTTCACCCCTTAAACAAAGTCAGAGAGGAATTTAGACAAATT TTCTTTTCCATGGGATTCACAGAGATGCCCTCGAACCAATACGTCGAGACAGGTTTCTGG AACTTCGATGCCCTTTACGTCCCACAACAGCATCCTGCTCGTGACCTGCAAGACACTTTC TACATCAAGGACCCACTAACCGCTGACTTGCCCGATGACAAGACATACATGGACAATATC AAAGCCGTTCACGAACAGGGGAGATTCGGGTCCATCGGTTATCGTTACAACTGGAAGCCA GAAGAATGTCAAAAATTGGTCTTGAGAACTCACTCCACAGCCATCTCTGCCAGAATGCTG CACGATTTGGCCAAAGATCCAAAGCCCACCAGATTGTTTTCTATCGACCGTGTTTTCCGT AACGAAGCAGTTGACGCCACCCATTTGGCCGAATTCCACCAGGTGGAAGGTGTTCTTGCC GACTACAACATTACTCTGGGTGACCTGATCAAGTTCATGGAAGAGTTTTTCGAAAGAATG GGTGTCACCGGTTTGAGATTCAAGCCTACCTACAATCCTTACACCGAGCCATCAATGGAA ATCTTTTCTTGGCACGAAGGTTTGCAAAAATGGGTCGAAATCGGTAACTCTGGTATGTTC AGACCAGAAATGCTCGAGTCCATGGGTCTACCAAAGGATCTAAGAGTCCTTGGTTGGGGG TTATCCTTGGAAAGACCTACCATGATCAAATATAAGGTTCAAAACATCAGAGAACTGTTA GGTCATAAAGTCTCTTTGGACTTTATCGAAACCAATCCTGCTGCTAGATTGGACGAAGAC TTGTACGAATAA
Protein Properties
Pfam Domain Function
Protein Residues503
Protein Molecular Weight57511.0
Protein Theoretical pI5.53
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Phenylalanyl-tRNA synthetase alpha chain MSDFQLEILKKLDELDEIKSTLATFPQHGSQDVLSALNSLKAHNKLEFSKVDTVTYDLTK EGAQILNEGSYEIKLVKLIQELGQLQIKDVMSKLGPQVGKVGQARAFKNGWIAKNASNEL ELSAKLQNTDLNELTDETQSILAQIKNNSHLDSIDAKILNDLKKRKLIAQGKITDFNVTK GPEFSTDLTKLETDLTSDMVSTNAYKDLKFKPYNFNSQGVQISSGALHPLNKVREEFRQI FFSMGFTEMPSNQYVETGFWNFDALYVPQQHPARDLQDTFYIKDPLTADLPDDKTYMDNI KAVHEQGRFGSIGYRYNWKPEECQKLVLRTHSTAISARMLHDLAKDPKPTRLFSIDRVFR NEAVDATHLAEFHQVEGVLADYNITLGDLIKFMEEFFERMGVTGLRFKPTYNPYTEPSME IFSWHEGLQKWVEIGNSGMFRPEMLESMGLPKDLRVLGWGLSLERPTMIKYKVQNIRELL GHKVSLDFIETNPAARLDEDLYE
References
External Links
ResourceLink
Saccharomyces Genome Database FRS2
Uniprot IDP15625
Uniprot NameSYFA_YEAST
GenBank Gene IDD50617
Genebank Protein ID836732
General Reference
  • Sanni, A., Mirande, M., Ebel, J. P., Boulanger, Y., Waller, J. P., Fasiolo, F. (1988). "Structure and expression of the genes encoding the alpha and beta subunits of yeast phenylalanyl-tRNA synthetase." J Biol Chem 263:15407-15415.3049607
  • Murakami, Y., Naitou, M., Hagiwara, H., Shibata, T., Ozawa, M., Sasanuma, S., Sasanuma, M., Tsuchiya, Y., Soeda, E., Yokoyama, K., et, a. l. .. (1995). "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Nat Genet 10:261-268.7670463
  • Fasiolo, F., Sanni, A., Potier, S., Ebel, J. P., Boulanger, Y. (1989). "Identification of the major tRNA(Phe) binding domain in the tetrameric structure of cytoplasmic phenylalanyl-tRNA synthetase from baker's yeast." FEBS Lett 242:351-356.2644133
  • Garrels, J. I., McLaughlin, C. S., Warner, J. R., Futcher, B., Latter, G. I., Kobayashi, R., Schwender, B., Volpe, T., Anderson, D. S., Mesquita-Fuentes, R., Payne, W. E. (1997). "Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins." Electrophoresis 18:1347-1360.9298649
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956