Isopentenyl-diphosphate Delta-isomerase (P15496)

Identification
NameIsopentenyl-diphosphate Delta-isomerase
Synonyms
  • Isopentenyl pyrophosphate isomerase
  • IPP isomerase
Gene NameIDI1
Enzyme Class
Biological Properties
General FunctionInvolved in isopentenyl-diphosphate delta-isomerase activity
Specific FunctionCatalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP)
Cellular LocationCytoplasm
SMPDB Pathways
Cholesterol biosynthesis and metabolism CE(10:0)PW002545 ThumbThumb?image type=greyscaleThumb?image type=simple
Cholesterol biosynthesis and metabolism CE(12:0)PW002548 ThumbThumb?image type=greyscaleThumb?image type=simple
Cholesterol biosynthesis and metabolism CE(14:0)PW002544 ThumbThumb?image type=greyscaleThumb?image type=simple
Cholesterol biosynthesis and metabolism CE(16:0)PW002550 ThumbThumb?image type=greyscaleThumb?image type=simple
Cholesterol biosynthesis and metabolism CE(18:0)PW002551 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Terpenoid backbone biosynthesisec00900 Map00900
SMPDB Reactions
Isopentenyl pyrophosphate ↔ Dimethylallylpyrophosphate
Isopentenyl pyrophosphate → Dimethylallylpyrophosphate
KEGG Reactions
Isopentenyl pyrophosphate ↔ Dimethylallylpyrophosphate
Metabolites
YMDB IDNameView
YMDB00095DimethylallylpyrophosphateShow
YMDB00200Isopentenyl pyrophosphateShow
GO Classification
Component
Not Available
Function
hydrolase activity
isomerase activity
intramolecular oxidoreductase activity
intramolecular oxidoreductase activity, transposing C=C bonds
isopentenyl-diphosphate delta-isomerase activity
catalytic activity
Process
primary metabolic process
lipid metabolic process
cellular lipid metabolic process
isoprenoid metabolic process
isoprenoid biosynthetic process
metabolic process
Gene Properties
Chromosome Locationchromosome 16
LocusYPL117C
Gene Sequence>867 bp ATGACTGCCGACAACAATAGTATGCCCCATGGTGCAGTATCTAGTTACGCCAAATTAGTG CAAAACCAAACACCTGAAGACATTTTGGAAGAGTTTCCTGAAATTATTCCATTACAACAA AGACCTAATACCCGATCTAGTGAGACGTCAAATGACGAAAGCGGAGAAACATGTTTTTCT GGTCATGATGAGGAGCAAATTAAGTTAATGAATGAAAATTGTATTGTTTTGGATTGGGAC GATAATGCTATTGGTGCCGGTACCAAGAAAGTTTGTCATTTAATGGAAAATATTGAAAAG GGTTTACTACATCGTGCATTCTCCGTCTTTATTTTCAATGAACAAGGTGAATTACTTTTA CAACAAAGAGCCACTGAAAAAATAACTTTCCCTGATCTTTGGACTAACACATGCTGCTCT CATCCACTATGTATTGATGACGAATTAGGTTTGAAGGGTAAGCTAGACGATAAGATTAAG GGCGCTATTACTGCGGCGGTGAGAAAACTAGATCATGAATTAGGTATTCCAGAAGATGAA ACTAAGACAAGGGGTAAGTTTCACTTTTTAAACAGAATCCATTACATGGCACCAAGCAAT GAACCATGGGGTGAACATGAAATTGATTACATCCTATTTTATAAGATCAACGCTAAAGAA AACTTGACTGTCAACCCAAACGTCAATGAAGTTAGAGACTTCAAATGGGTTTCACCAAAT GATTTGAAAACTATGTTTGCTGACCCAAGTTACAAGTTTACGCCTTGGTTTAAGATTATT TGCGAGAATTACTTATTCAACTGGTGGGAGCAATTAGATGACCTTTCTGAAGTGGAAAAT GACAGGCAAATTCATAGAATGCTATAA
Protein Properties
Pfam Domain Function
Protein Residues288
Protein Molecular Weight33351.30078
Protein Theoretical pI4.61
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Isopentenyl-diphosphate Delta-isomerase MTADNNSMPHGAVSSYAKLVQNQTPEDILEEFPEIIPLQQRPNTRSSETSNDESGETCFS GHDEEQIKLMNENCIVLDWDDNAIGAGTKKVCHLMENIEKGLLHRAFSVFIFNEQGELLL QQRATEKITFPDLWTNTCCSHPLCIDDELGLKGKLDDKIKGAITAAVRKLDHELGIPEDE TKTRGKFHFLNRIHYMAPSNEPWGEHEIDYILFYKINAKENLTVNPNVNEVRDFKWVSPN DLKTMFADPSYKFTPWFKIICENYLFNWWEQLDDLSEVENDRQIHRML
References
External Links
ResourceLink
Saccharomyces Genome Database IDI1
Uniprot IDP15496
Uniprot NameIDI1_YEAST
GenBank Gene IDJ05090
Genebank Protein ID171760
General Reference
  • Anderson, M. S., Muehlbacher, M., Street, I. P., Proffitt, J., Poulter, C. D. (1989). "Isopentenyl diphosphate:dimethylallyl diphosphate isomerase. An improved purification of the enzyme and isolation of the gene from Saccharomyces cerevisiae." J Biol Chem 264:19169-19175.2681212
  • Bussey, H., Storms, R. K., Ahmed, A., Albermann, K., Allen, E., Ansorge, W., Araujo, R., Aparicio, A., Barrell, B., Badcock, K., Benes, V., Botstein, D., Bowman, S., Bruckner, M., Carpenter, J., Cherry, J. M., Chung, E., Churcher, C., Coster, F., Davis, K., Davis, R. W., Dietrich, F. S., Delius, H., DiPaolo, T., Hani, J., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Nature 387:103-105.9169875
  • Street, I. P., Poulter, C. D. (1990). "Isopentenyldiphosphate:dimethylallyldiphosphate isomerase: construction of a high-level heterologous expression system for the gene from Saccharomyces cerevisiae and identification of an active-site nucleophile." Biochemistry 29:7531-7538.2223785
  • Street, I. P., Coffman, H. R., Baker, J. A., Poulter, C. D. (1994). "Identification of Cys139 and Glu207 as catalytically important groups in the active site of isopentenyl diphosphate:dimethylallyl diphosphate isomerase." Biochemistry 33:4212-4217.7908830
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Smolka, M. B., Albuquerque, C. P., Chen, S. H., Zhou, H. (2007). "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Proc Natl Acad Sci U S A 104:10364-10369.17563356
  • Albuquerque, C. P., Smolka, M. B., Payne, S. H., Bafna, V., Eng, J., Zhou, H. (2008). "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Mol Cell Proteomics 7:1389-1396.18407956