Dolichol-phosphate mannosyltransferase (P14020)

Identification
NameDolichol-phosphate mannosyltransferase
Synonyms
  • Dolichol-phosphate mannose synthase
  • DPM synthase
  • Dolichyl-phosphate beta-D-mannosyltransferase
  • Mannose-P-dolichol synthase
  • MPD synthase
Gene NameDPM1
Enzyme Class
Biological Properties
General FunctionCell wall/membrane/envelope biogenesis
Specific FunctionTransfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O- mannosylation of proteins
Cellular LocationEndoplasmic reticulum membrane; Single-pass type IV membrane protein
SMPDB Pathways
N-Glycan biosynthesisPW002501 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
N-Glycan biosynthesisec00510 Map00510
SMPDB Reactions
Guanosine diphosphate mannose + Dolichol phosphate → Guanosine diphosphate + dolichyl β-D-mannosyl phosphate
KEGG Reactions
dolichyl phosphate + Guanosine diphosphate mannose → Guanosine diphosphate + dolichyl D-mannosyl phosphate
Metabolites
YMDB IDNameView
YMDB00202Guanosine diphosphate mannoseShow
YMDB00257Guanosine diphosphateShow
YMDB00864Dolichol phosphateShow
YMDB00928Dolichol-20Show
YMDB00937dolichyl D-mannosyl phosphateShow
YMDB00941dolichyl phosphateShow
YMDB16337dolichyl β-D-mannosyl phosphateShow
GO ClassificationNot Available
Gene Properties
Chromosome Locationchromosome 16
LocusYPR183W
Gene Sequence>804 bp ATGAGCATCGAATACTCTGTTATCGTTCCCGCTTACCATGAAAAGCTGAACATCAAACCC TTAACAACCAGATTGTTTGCCGGTATGAGCCCTGAAATGGCCAAGAAGACCGAGTTGATC TTTGTCGATGACAACTCACAGGATGGCTCCGTTGAGGAGGTGGACGCCCTAGCTCACCAA GGCTACAACGTCCGCATCATCGTCAGGACAAATGAACGTGGTCTATCCTCCGCCGTGCTT AAGGGTTTCTACGAGGCCAAGGGTCAGTACCTGGTATGCATGGACGCAGACCTACAACAC CCTCCAGAGACCGTGCCCAAGTTGTTCGAGTCTTTGCACGATCACGCCTTTACTCTGGGC ACCAGATATGCCCCAGGTGTCGGCATTGACAAGGACTGGCCTATGTACAGACGCGTCATT TCCTCCACTGCTAGAATGATGGCCAGACCTTTGACCATCGCCTCCGACCCCATGAGCGGG TTCTTCGGTCTACAAAAGAAGTACCTCGAAAACTGCAACCCTAGGGACATCAACTCGCAA GGTTTCAAGATTGCCCTCGAGCTTCTCGCCAAGCTTCCTCTTCCAAGAGACCCTAGAGTG GCGATCGGCGAAGTGCCATTCACTTTCGGCGTGAGAACGGAAGGTGAATCCAAACTGTCA GGTAAAGTCATTATCCAATACTTGCAACAACTTAAGGAGCTCTACGTCTTCAAGTTTGGC GCCAATAACCTTATCCTTTTCATTACTTTCTGGTCCATTCTGTTCTTCTACGTTTGCTAC CAGCTATACCATTTGGTCTTTTAA
Protein Properties
Pfam Domain Function
Protein Residues267
Protein Molecular Weight30362.0
Protein Theoretical pI8.06
Signalling Regions
  • None
Transmembrane Regions
  • 239-259
Protein Sequence>Dolichol-phosphate mannosyltransferase MSIEYSVIVPAYHEKLNIKPLTTRLFAGMSPEMAKKTELIFVDDNSQDGSVEEVDALAHQ GYNVRIIVRTNERGLSSAVLKGFYEAKGQYLVCMDADLQHPPETVPKLFESLHDHAFTLG TRYAPGVGIDKDWPMYRRVISSTARMMARPLTIASDPMSGFFGLQKKYLENCNPRDINSQ GFKIALELLAKLPLPRDPRVAIGEVPFTFGVRTEGESKLSGKVIIQYLQQLKELYVFKFG ANNLILFITFWSILFFYVCYQLYHLVF
References
External Links
ResourceLink
Saccharomyces Genome Database DPM1
Uniprot IDP14020
Uniprot NameDPM1_YEAST
GenBank Gene IDJ04184
Genebank Protein ID171418
General Reference
  • Orlean, P., Albright, C., Robbins, P. W. (1988). "Cloning and sequencing of the yeast gene for dolichol phosphate mannose synthase, an essential protein." J Biol Chem 263:17499-17507.3053713
  • Bussey, H., Storms, R. K., Ahmed, A., Albermann, K., Allen, E., Ansorge, W., Araujo, R., Aparicio, A., Barrell, B., Badcock, K., Benes, V., Botstein, D., Bowman, S., Bruckner, M., Carpenter, J., Cherry, J. M., Chung, E., Churcher, C., Coster, F., Davis, K., Davis, R. W., Dietrich, F. S., Delius, H., DiPaolo, T., Hani, J., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Nature 387:103-105.9169875
  • Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., O'Shea, E. K. (2003). "Global analysis of protein localization in budding yeast." Nature 425:686-691.14562095
  • Ghaemmaghami, S., Huh, W. K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., Weissman, J. S. (2003). "Global analysis of protein expression in yeast." Nature 425:737-741.14562106
  • Faulhammer, F., Konrad, G., Brankatschk, B., Tahirovic, S., Knodler, A., Mayinger, P. (2005). "Cell growth-dependent coordination of lipid signaling and glycosylation is mediated by interactions between Sac1p and Dpm1p." J Cell Biol 168:185-191.15657391
  • Banerjee, D. K., Carrasquillo, E. A., Hughey, P., Schutzbach, J. S., Martinez, J. A., Baksi, K. (2005). "In vitro phosphorylation by cAMP-dependent protein kinase up-regulates recombinant Saccharomyces cerevisiae mannosylphosphodolichol synthase." J Biol Chem 280:4174-4181.15548536