Cytochrome b-c1 complex subunit Rieske, mitochondrial (P08067)

Identification
NameCytochrome b-c1 complex subunit Rieske, mitochondrial
Synonyms
  • Complex III subunit 5
  • Rieske iron-sulfur protein
  • RISP
  • Ubiquinol-cytochrome c reductase iron-sulfur subunit
Gene NameRIP1
Enzyme Class
Biological Properties
General FunctionInvolved in oxidoreductase activity
Specific FunctionComponent of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The complex couples electron transfer from ubiquinol to cytochrome c
Cellular LocationMitochondrion
SMPDB Pathways
Oxidative phosphorylationPW002461 ThumbThumb?image type=greyscaleThumb?image type=simple
KEGG Pathways
Oxidative phosphorylationec00190 Map00190
SMPDB ReactionsNot Available
KEGG ReactionsNot Available
Metabolites
YMDB IDNameView
YMDB00088Ubiquinone Q1Show
YMDB00862hydronShow
YMDB00902ubiquinolShow
GO Classification
Component
cell part
membrane
Function
ion transmembrane transporter activity
cation transmembrane transporter activity
inorganic cation transmembrane transporter activity
monovalent inorganic cation transmembrane transporter activity
catalytic activity
hydrogen ion transmembrane transporter activity
2 iron, 2 sulfur cluster binding
ubiquinol-cytochrome-c reductase activity
oxidoreductase activity, acting on diphenols and related substances as donors
binding
metal cluster binding
iron-sulfur cluster binding
oxidoreductase activity
transporter activity
transmembrane transporter activity
substrate-specific transmembrane transporter activity
Process
metabolic process
oxidation reduction
Gene Properties
Chromosome Locationchromosome 5
LocusYEL024W
Gene Sequence>648 bp ATGTTAGGAATAAGATCATCTGTCAAGACCTGCTTCAAGCCAATGTCTTTAACTTCCAAA AGACTGATTTCTCAATCTTTGCTGGCTAGCAAATCCACGTACAGGACCCCAAATTTTGAT GACGTTTTAAAGGAAAATAACGATGCTGATAAAGGCCGTTCTTATGCTTACTTTATGGTC GGTGCTATGGGTCTTTTGTCATCTGCAGGTGCCAAATCAACCGTAGAAACCTTTATTTCA TCTATGACCGCTACTGCCGATGTTTTGGCTATGGCTAAAGTTGAAGTTAATTTAGCGGCT ATCCCATTGGGTAAAAACGTGGTTGTCAAATGGCAAGGTAAGCCTGTGTTCATTAGACAC AGAACTCCTCATGAAATTCAGGAAGCCAACAGTGTGGATATGTCCGCTTTGAAGGACCCA CAGACCGATGCTGACAGAGTCAAAGACCCTCAATGGTTAATTATGCTGGGTATTTGTACT CACTTAGGTTGTGTTCCAATTGGTGAAGCCGGTGATTTTGGTGGTTGGTTCTGTCCTTGC CATGGTTCACATTATGATATTTCCGGTAGAATCAGAAAGGGACCTGCCCCCTTAAACTTA GAAATTCCTGCTTATGAATTCGATGGTGATAAGGTCATTGTTGGTTAG
Protein Properties
Pfam Domain Function
Protein Residues215
Protein Molecular Weight23364.69922
Protein Theoretical pI8.21
PDB Fileshow
Signalling Regions
  • None
Transmembrane Regions
  • None
Protein Sequence>Cytochrome b-c1 complex subunit Rieske, mitochondrial MLGIRSSVKTCFKPMSLTSKRLISQSLLASKSTYRTPNFDDVLKENNDADKGRSYAYFMV GAMGLLSSAGAKSTVETFISSMTATADVLAMAKVEVNLAAIPLGKNVVVKWQGKPVFIRH RTPHEIQEANSVDMSALKDPQTDADRVKDPQWLIMLGICTHLGCVPIGEAGDFGGWFCPC HGSHYDISGRIRKGPAPLNLEIPAYEFDGDKVIVG
References
External Links
ResourceLink
Saccharomyces Genome Database RIP1
Uniprot IDP08067
Uniprot NameUCRI_YEAST
GenBank Gene IDAY558341
Genebank Protein ID45270572
PDB ID
1P84
General Reference
  • Beckmann, J. D., Ljungdahl, P. O., Lopez, J. L., Trumpower, B. L. (1987). "Isolation and characterization of the nuclear gene encoding the Rieske iron-sulfur protein (RIP1) from Saccharomyces cerevisiae." J Biol Chem 262:8901-8909.3036836
  • Beckmann, J. D., Ljungdahl, P. O., Trumpower, B. L. (1989). "Mutational analysis of the mitochondrial Rieske iron-sulfur protein of Saccharomyces cerevisiae. I. Construction of a RIP1 deletion strain and isolation of temperature-sensitive mutants." J Biol Chem 264:3713-3722.2645276
  • Dietrich, F. S., Mulligan, J., Hennessy, K., Yelton, M. A., Allen, E., Araujo, R., Aviles, E., Berno, A., Brennan, T., Carpenter, J., Chen, E., Cherry, J. M., Chung, E., Duncan, M., Guzman, E., Hartzell, G., Hunicke-Smith, S., Hyman, R. W., Kayser, A., Komp, C., Lashkari, D., Lew, H., Lin, D., Mosedale, D., Davis, R. W., et, a. l. .. (1997). "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Nature 387:78-81.9169868
  • Hu, Y., Rolfs, A., Bhullar, B., Murthy, T. V., Zhu, C., Berger, M. F., Camargo, A. A., Kelley, F., McCarron, S., Jepson, D., Richardson, A., Raphael, J., Moreira, D., Taycher, E., Zuo, D., Mohr, S., Kane, M. F., Williamson, J., Simpson, A., Bulyk, M. L., Harlow, E., Marsischky, G., Kolodner, R. D., LaBaer, J. (2007). "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Genome Res 17:536-543.17322287
  • Graham, L. A., Trumpower, B. L. (1991). "Mutational analysis of the mitochondrial Rieske iron-sulfur protein of Saccharomyces cerevisiae. III. Import, protease processing, and assembly into the cytochrome bc1 complex of iron-sulfur protein lacking the iron-sulfur cluster." J Biol Chem 266:22485-22492.1657998
  • Gatti, D. L., Meinhardt, S. W., Ohnishi, T., Tzagoloff, A. (1989). "Structure and function of the mitochondrial bc1 complex. A mutational analysis of the yeast Rieske iron-sulfur protein." J Mol Biol 205:421-435.2538628
  • Hunte, C., Koepke, J., Lange, C., Rossmanith, T., Michel, H. (2000). "Structure at 2.3 A resolution of the cytochrome bc(1) complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment." Structure 8:669-684.10873857
  • Lange, C., Hunte, C. (2002). "Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c." Proc Natl Acad Sci U S A 99:2800-2805.11880631